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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
11/05/2020 |
Data da última atualização: |
11/05/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FAJARDO, T. V. M.; Bertocchi, A. A.; NICKEL, O. |
Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV. |
Título: |
Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Revista Ceres, Viçosa, v. 67, n. 2, p. 156-163, mar./apr. 2020. |
DOI: |
10.1590/0034-737X202067020010 |
Idioma: |
Inglês |
Conteúdo: |
Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey |
Palavras-Chave: |
Grapevine (Vitis spp); HTS; Indexing; NGS; RT-qPCR; Survey. |
Thesaurus Nal: |
Pathogens; Viruses; Vitis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212854/1/Rev-Ceres-Determination-grapevine-virome-HTS-detection-Serra-Gaucha-v67n2-2020.pdf
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Marc: |
LEADER 01779naa a2200265 a 4500 001 2122194 005 2020-05-11 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1590/0034-737X202067020010$2DOI 100 1 $aFAJARDO, T. V. M. 245 $aDetermination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.$h[electronic resource] 260 $c2020 520 $aAmong grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey 650 $aPathogens 650 $aViruses 650 $aVitis 653 $aGrapevine (Vitis spp) 653 $aHTS 653 $aIndexing 653 $aNGS 653 $aRT-qPCR 653 $aSurvey 700 1 $aBertocchi, A. A. 700 1 $aNICKEL, O. 773 $tRevista Ceres, Viçosa$gv. 67, n. 2, p. 156-163, mar./apr. 2020.
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