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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
24/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PARAENSE, L. C. R.; PENA, D. N.; DARNET, S. H.; RODRIGUES, S. de M.; MENEZES, I. C. de; CUNHA, E. F. M. |
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Afiliação: |
LUANY CAROLINE RIBEIRO PARAENSE, UFRA; DAYANE NASCIMENTO PENA, UFRA; SYLVAIN HENRI DARNET, UFPA; SIMONE DE MIRANDA RODRIGUES, CPATU; ILMARINA CAMPOS DE MENEZES, CPATU; ELISA FERREIRA MOURA CUNHA, CPATU. |
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Título: |
First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 47, n. 4, p. 2985-2989, fev. 2020. |
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DOI: |
https://doi.org/10.1007/s11033-020-05301-0 |
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Idioma: |
Inglês |
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Notas: |
Short Comunication. |
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Conteúdo: |
Platonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (HE) varied from 0.74 (PI27) to 0.12 (PI31)b and observed heterozygosity (HO) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations. |
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Palavras-Chave: |
Diversidade genética; Genetic diversity; Next generation sequencing; Sequenciamento genético; Simple sequence repeats. |
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Thesagro: |
Bacuri; Platonia Insignis. |
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Thesaurus Nal: |
Clusiaceae; Oligonucleotides. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211920/1/Paraense2020-Article-FirstGenomicMicrosatelliteMark.pdf
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Marc: |
LEADER 02375naa a2200313 a 4500
001 2121255
005 2021-05-24
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11033-020-05301-0$2DOI
100 1 $aPARAENSE, L. C. R.
245 $aFirst genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree.$h[electronic resource]
260 $c2020
500 $aShort Comunication.
520 $aPlatonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (HE) varied from 0.74 (PI27) to 0.12 (PI31)b and observed heterozygosity (HO) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations.
650 $aClusiaceae
650 $aOligonucleotides
650 $aBacuri
650 $aPlatonia Insignis
653 $aDiversidade genética
653 $aGenetic diversity
653 $aNext generation sequencing
653 $aSequenciamento genético
653 $aSimple sequence repeats
700 1 $aPENA, D. N.
700 1 $aDARNET, S. H.
700 1 $aRODRIGUES, S. de M.
700 1 $aMENEZES, I. C. de
700 1 $aCUNHA, E. F. M.
773 $tMolecular Biology Reports$gv. 47, n. 4, p. 2985-2989, fev. 2020.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| 1. |  | PARAENSE, L. C. R.; PENA, D. N.; DARNET, S. H.; RODRIGUES, S. de M.; MENEZES, I. C. de; CUNHA, E. F. M. First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree. Molecular Biology Reports, v. 47, n. 4, p. 2985-2989, fev. 2020. Short Comunication.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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