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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
04/03/2020 |
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Data da última atualização: |
04/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARPENTIERI-PIPOLO, V.; LOPES, K. B. de A. L.; DEGRASSI, G. |
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Afiliação: |
VALERIA CARPENTIERI PIPOLO, CNPT; Karla Bianca de Almeida Lopes, Agronomy Department, Londrina State University, Rodovia Celso Garcia Cid, Pr 445 km 380, PO Box 10.011, Londrina, PR 86057-970, Brazil; Giuliano Degrassi, IBioBA-ICGEB, International Centre for Genetic Engineering and Biotechnology, Polo Cientifco Tecnologico, Godoy Cruz 2390, C1425FQD Buenos Aires, Argentina. |
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Título: |
Phenotypic and genotypic characterization of endophytic bacteria associated with transgenic and non-transgenic soybean plants. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Archives of Microbiology, N. 201, P. 1029-1045, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Endophytic bacteria isolated from non-transgenic and transgenic Roundup Ready® glyphosate-resistant (GR) soybean plants were investigated to analyze the correspondence between phenotypic and genotypic characteristics and to determine whether or not the strains could be grouped based on the source of isolation in transgenic or non-transgenic plants, respectively. Most of the strains recovered from GR plants have shown the ability for plant growth promotion (PGP) by means of IAA production and inorganic phosphate solubilization, and 100% of the strains showed great motility (swarm or swim); in addition, 90% of the strains were able to metabolize the majority of carbon sources tested. GR soybean felds showed higher endophytes abundance than non-transgenic; however, analyzing the phylogenetic trees constructed using the partial 16SrRNA gene sequences, higher diversity was observed in non-transgenic soybean felds. Overall the majority of isolated endophytes could utilize multiple patterns of carbon sources and express resistance to antibiotics, while isolates varied widely in the PGP ability. The greater pattern and frequency of utilization of carbon sources and frequency and intensity of antibiotic resistance compared with PGP ability within the soybean endophytes community suggest that carbon sources metabolism and antibiotic resistance confer a greater relative ftness beneft than PGP ability. In conclusion, cluster analysis of the phenotypes and 16SrRNA gene sequences reveals lack of correspondence between the pattern of bacterial isolates and the transgenic character of plants, and the heterogeneity of clustering suggested that various adaptive processes, such as stress response, could have contributed to generate phenotypic variability to enhance endophytes overall ftness. Keywords Transgenic soybean · Endophytic bacteria · 16SrRNA · Antibiotics · Carbon sources metabolism · Plant growth promotion MenosEndophytic bacteria isolated from non-transgenic and transgenic Roundup Ready® glyphosate-resistant (GR) soybean plants were investigated to analyze the correspondence between phenotypic and genotypic characteristics and to determine whether or not the strains could be grouped based on the source of isolation in transgenic or non-transgenic plants, respectively. Most of the strains recovered from GR plants have shown the ability for plant growth promotion (PGP) by means of IAA production and inorganic phosphate solubilization, and 100% of the strains showed great motility (swarm or swim); in addition, 90% of the strains were able to metabolize the majority of carbon sources tested. GR soybean felds showed higher endophytes abundance than non-transgenic; however, analyzing the phylogenetic trees constructed using the partial 16SrRNA gene sequences, higher diversity was observed in non-transgenic soybean felds. Overall the majority of isolated endophytes could utilize multiple patterns of carbon sources and express resistance to antibiotics, while isolates varied widely in the PGP ability. The greater pattern and frequency of utilization of carbon sources and frequency and intensity of antibiotic resistance compared with PGP ability within the soybean endophytes community suggest that carbon sources metabolism and antibiotic resistance confer a greater relative ftness beneft than PGP ability. In conclusion, cluster analysis of the phenotypes and 16SrRNA gene sequences reveals ... Mostrar Tudo |
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Palavras-Chave: |
16SrRNA; Carbon sources metabolism; Endophytic bacteria; Plant growth promotion; Transgenic soybean. |
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Thesaurus Nal: |
Antibiotics; Soybeans. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211499/1/ID44836-2019v201p1029ArchMicrobiol.pdf
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Marc: |
LEADER 02667naa a2200229 a 4500
001 2120913
005 2020-03-04
008 2019 bl uuuu u00u1 u #d
100 1 $aCARPENTIERI-PIPOLO, V.
245 $aPhenotypic and genotypic characterization of endophytic bacteria associated with transgenic and non-transgenic soybean plants.$h[electronic resource]
260 $c2019
520 $aEndophytic bacteria isolated from non-transgenic and transgenic Roundup Ready® glyphosate-resistant (GR) soybean plants were investigated to analyze the correspondence between phenotypic and genotypic characteristics and to determine whether or not the strains could be grouped based on the source of isolation in transgenic or non-transgenic plants, respectively. Most of the strains recovered from GR plants have shown the ability for plant growth promotion (PGP) by means of IAA production and inorganic phosphate solubilization, and 100% of the strains showed great motility (swarm or swim); in addition, 90% of the strains were able to metabolize the majority of carbon sources tested. GR soybean felds showed higher endophytes abundance than non-transgenic; however, analyzing the phylogenetic trees constructed using the partial 16SrRNA gene sequences, higher diversity was observed in non-transgenic soybean felds. Overall the majority of isolated endophytes could utilize multiple patterns of carbon sources and express resistance to antibiotics, while isolates varied widely in the PGP ability. The greater pattern and frequency of utilization of carbon sources and frequency and intensity of antibiotic resistance compared with PGP ability within the soybean endophytes community suggest that carbon sources metabolism and antibiotic resistance confer a greater relative ftness beneft than PGP ability. In conclusion, cluster analysis of the phenotypes and 16SrRNA gene sequences reveals lack of correspondence between the pattern of bacterial isolates and the transgenic character of plants, and the heterogeneity of clustering suggested that various adaptive processes, such as stress response, could have contributed to generate phenotypic variability to enhance endophytes overall ftness. Keywords Transgenic soybean · Endophytic bacteria · 16SrRNA · Antibiotics · Carbon sources metabolism · Plant growth promotion
650 $aAntibiotics
650 $aSoybeans
653 $a16SrRNA
653 $aCarbon sources metabolism
653 $aEndophytic bacteria
653 $aPlant growth promotion
653 $aTransgenic soybean
700 1 $aLOPES, K. B. de A. L.
700 1 $aDEGRASSI, G.
773 $tArchives of Microbiology, N. 201, P. 1029-1045, 2019.
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