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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/02/2020 |
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Data da última atualização: |
08/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da. |
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Afiliação: |
TATIANA MARIA FARIAS PINTO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; RAULZITO FERNANDES MOREIRA, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MARIA NAGILA CARNEIRO MATOS, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; VITÓRIA VIRGINIA MAGALHÃES SOARES, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; MÔNICA VALERIA DE ALMEIDA AGUIAR, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; PAULO DE TARSO TELES DOURADO DE ARAGÃO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; JOÃO GARCIA ALVES FILHO, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; FREDERICO BRUNO MENDES BATISTA MORENO, Universidade de Fortaleza (UNIFOR) - Fortaleza, CE, Brasil; ANA CRISTINA DE OLIVEIRA MONTEIRO-MOREIRA, Universidade de Fortaleza (UNIFOR) - Fortaleza, CE, Brasil; CÍNTIA RENATA ROCHA COSTA, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil; JOSÉ LUIZ DE LIMA FILHO, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil; ANGELA MARIA XAVIER ELOY, CNPC; RODRIGO MARANGUAPE SILVA DA CUNHA, Universidade Federal de Pernambuco Federal (UFPE) - Recife, PE, Brasil. |
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Título: |
Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction, v. 16, n. 4, p. 902-913, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization. |
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Palavras-Chave: |
Brasil; Brazilian Northeast; Profiles; Proteomic profile; Sperm. |
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Thesagro: |
Caprino. |
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Thesaurus Nal: |
Goats; Proteomics; Saanen; Semiarid zones; Spermatogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/210674/1/CNPC-2019-Evaluation-of.pdf
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Marc: |
LEADER 02079naa a2200397 a 4500
001 2120171
005 2020-12-08
008 2019 bl uuuu u00u1 u #d
100 1 $aPINTO, T. M. F.
245 $aEvaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus).
260 $c2019
520 $aAbstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.
650 $aGoats
650 $aProteomics
650 $aSaanen
650 $aSemiarid zones
650 $aSpermatogenesis
650 $aCaprino
653 $aBrasil
653 $aBrazilian Northeast
653 $aProfiles
653 $aProteomic profile
653 $aSperm
700 1 $aMOREIRA, R. F.
700 1 $aMATOS, M. N. C.
700 1 $aSOARES, V. V. M.
700 1 $aAGUIAR, M. V. de A.
700 1 $aARAGÃO, P. de T. T. D. de
700 1 $aALVES FILHO, J. G.
700 1 $aMORENO, F. B. M. B.
700 1 $aMONTEIRO-MOREIRA, A. C. de O.
700 1 $aCOSTA, C. R. R.
700 1 $aLIMA FILHO, J. L. de
700 1 $aELOY, A. M. X.
700 1 $aCUNHA, R. M. S. da
773 $tAnimal Reproduction$gv. 16, n. 4, p. 902-913, 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da. Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). Animal Reproduction, v. 16, n. 4, p. 902-913, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 1 | |
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