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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, F. G.; COSTA, E. P. DA; QUEIROZ-CASTRO, V. L. D.; PEREIRA, E. C. M.; GUIMARÃES, J. D.; ALVES, S. V. P.; FERNANDES, C. A. C.; CAMARGO, L. S. de A.; BENJAMIM, L. DOS A. |
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Afiliação: |
Flavio Guisseli Lopes; Eduardo Paulino da Costa; Vanessa Lopes Dias Queiroz-Castro; Emilio Cesar Martins Pereira; José Domingos Guimarães; Saullo Vinicius Pereira Alves; Carlos Antonio Carvalho Fernandes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Laercio dos Anjos Benjamim. |
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Título: |
Use of two new formulations as bovine embryo manipulation solution. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction, v. 16 n. 2, p. 348-355, 2019. |
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DOI: |
10.21451/1984-3143-ar2018-0044 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability. MenosABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS ... Mostrar Tudo |
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Palavras-Chave: |
Cell apoptosis; Hsp 70 ponto 3. |
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Thesaurus Nal: |
Mice. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208745/1/Use-of-two-new-formulations.pdf
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Marc: |
LEADER 02538naa a2200265 a 4500
001 2118720
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.21451/1984-3143-ar2018-0044$2DOI
100 1 $aLOPES, F. G.
245 $aUse of two new formulations as bovine embryo manipulation solution.$h[electronic resource]
260 $c2019
520 $aABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability.
650 $aMice
653 $aCell apoptosis
653 $aHsp 70 ponto 3
700 1 $aCOSTA, E. P. DA
700 1 $aQUEIROZ-CASTRO, V. L. D.
700 1 $aPEREIRA, E. C. M.
700 1 $aGUIMARÃES, J. D.
700 1 $aALVES, S. V. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBENJAMIM, L. DOS A.
773 $tAnimal Reproduction$gv. 16 n. 2, p. 348-355, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| 1. |  | LOPES, F. G.; COSTA, E. P. DA; QUEIROZ-CASTRO, V. L. D.; PEREIRA, E. C. M.; GUIMARÃES, J. D.; ALVES, S. V. P.; FERNANDES, C. A. C.; CAMARGO, L. S. de A.; BENJAMIM, L. DOS A. Use of two new formulations as bovine embryo manipulation solution. Animal Reproduction, v. 16 n. 2, p. 348-355, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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