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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
Data corrente: |
03/01/2020 |
Data da última atualização: |
03/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MORÉ, D. D.; CARDOSO, F. F.; MUDADU, M. de A.; MALAGO JUNIOR, W.; GULIAS GOMES, C. C.; SOLLERO, B. P.; IBELLI, A. M. G.; COUTINHO, L. L.; REGITANO, L. C. de A. |
Afiliação: |
Daniela D. Moré, CPPSE; FERNANDO FLORES CARDOSO, CPPSUL; MAURICIO DE ALVARENGA MUDADU, CNPTIA; WILSON MALAGO JUNIOR, CPPSE; CLAUDIA CRISTINA GULIAS GOMES, CPPSUL; BRUNA PENA SOLLERO, CPPSUL; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; Luiz L. Coutinho, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
Título: |
Network analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
BMC Genomics, v. 20, n. 998, 2019. |
DOI: |
10.1186/s12864-019-6360-3 |
Idioma: |
Inglês |
Conteúdo: |
Background: Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent
losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic
mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially
infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were
submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were
performed to identify genetic pathways and transcription factors (TFs) affecting host resistance.
Results: Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51
differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were
redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence
of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than
two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs
with potential anti-tick functions suggested a role in the development of different levels of resistance within the same
breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were
only found in TopDEGs from R hosts. Also, these genes indicated the participation of cytokines, such as IL6 and IL22,
and the activation of Wingless (WNT)-signaling pathway. A central gene of this pathway, WNT7A, was consistently
modulated when hosts were compared. Moreover, the findings based on a genome-wide association study (GWAS)
corroborate the prediction of the WNT-signaling pathway as a candidate mechanism of resistance. The regulation of
immune response was the most relevant pathway predicted for S hosts. Members of Ap1 and NF-kB families were the
most relevant TFs predicted for R and S, respectively.
Conclusion: This work provides indications of genetic mechanisms presented by Braford cattle with different levels of
resistance in response to tick infestation, contributing to the search of candidate genes for tick resistance in bovine. MenosBackground: Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent
losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic
mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially
infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were
submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were
performed to identify genetic pathways and transcription factors (TFs) affecting host resistance.
Results: Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51
differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were
redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence
of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than
two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs
with potential anti-tick functions suggested a role in the development of different levels of resistance within the same
breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were
only found in TopDEGs from... Mostrar Tudo |
Palavras-Chave: |
Bovine; Braford; Enrichment analysis; Expressão gênica; Gado Braford; Network analysis; RNA-Seq. |
Thesagro: |
Bovino; Carrapato; Resistência; Resistência Genética. |
Thesaurus Nal: |
Cattle; Gene expression; Rhipicephalus microplus; Tick infestations. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208137/1/More-et-al-2019.pdf
|
Marc: |
LEADER 03479naa a2200409 a 4500 001 2118135 005 2020-01-03 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1186/s12864-019-6360-3$2DOI 100 1 $aMORÉ, D. D. 245 $aNetwork analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin.$h[electronic resource] 260 $c2019 520 $aBackground: Genetic resistance in cattle is considered a suitable way to control tick burden and its consequent losses for livestock production. Exploring tick-resistant (R) and tick-susceptible (S) hosts, we investigated the genetic mechanisms underlying the variation of Braford resistance to tick infestation. Skin biopsies from four-times-artificially infested R (n = 20) and S (n = 19) hosts, obtained before the first and 24 h after the fourth tick infestation were submitted to RNA-Sequencing. Differential gene expression, functional enrichment, and network analysis were performed to identify genetic pathways and transcription factors (TFs) affecting host resistance. Results: Intergroup comparisons of hosts before (Rpre vs. Spre) and after (Rpost vs. Spost) tick infestation found 51 differentially expressed genes (DEGs), of which almost all presented high variation (TopDEGs), and 38 were redundant genes. Gene expression was consistently different between R and S hosts, suggesting the existence of specific anti-tick mechanisms. In the intragroup comparisons, Rpost vs. Rpre and Spost vs. Spre, we found more than two thousand DEGs in response to tick infestation in both resistance groups. Redundant and non-redundant TopDEGs with potential anti-tick functions suggested a role in the development of different levels of resistance within the same breed. Leukocyte chemotaxis was over-represented in both hosts, whereas skin degradation and remodeling were only found in TopDEGs from R hosts. Also, these genes indicated the participation of cytokines, such as IL6 and IL22, and the activation of Wingless (WNT)-signaling pathway. A central gene of this pathway, WNT7A, was consistently modulated when hosts were compared. Moreover, the findings based on a genome-wide association study (GWAS) corroborate the prediction of the WNT-signaling pathway as a candidate mechanism of resistance. The regulation of immune response was the most relevant pathway predicted for S hosts. Members of Ap1 and NF-kB families were the most relevant TFs predicted for R and S, respectively. Conclusion: This work provides indications of genetic mechanisms presented by Braford cattle with different levels of resistance in response to tick infestation, contributing to the search of candidate genes for tick resistance in bovine. 650 $aCattle 650 $aGene expression 650 $aRhipicephalus microplus 650 $aTick infestations 650 $aBovino 650 $aCarrapato 650 $aResistência 650 $aResistência Genética 653 $aBovine 653 $aBraford 653 $aEnrichment analysis 653 $aExpressão gênica 653 $aGado Braford 653 $aNetwork analysis 653 $aRNA-Seq 700 1 $aCARDOSO, F. F. 700 1 $aMUDADU, M. de A. 700 1 $aMALAGO JUNIOR, W. 700 1 $aGULIAS GOMES, C. C. 700 1 $aSOLLERO, B. P. 700 1 $aIBELLI, A. M. G. 700 1 $aCOUTINHO, L. L. 700 1 $aREGITANO, L. C. de A. 773 $tBMC Genomics$gv. 20, n. 998, 2019.
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Embrapa Pecuária Sul (CPPSUL) |
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1. | | MORÉ, D. D.; CARDOSO, F. F.; MUDADU, M. de A.; MALAGO JUNIOR, W.; GULIAS GOMES, C. C.; SOLLERO, B. P.; IBELLI, A. M. G.; COUTINHO, L. L.; REGITANO, L. C. de A. Network analysis uncovers putative genes affecting resistance to tick infestation in Braford cattle skin. BMC Genomics, v. 20, n. 998, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agricultura Digital; Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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