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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
27/12/2019 |
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Data da última atualização: |
27/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, I. I. F. de; RODRIGUES, R. de A.; JORGE, K. S. G.; SILVA, M. R.; LILENBAUM, W.; VIDAL, C. E. S.; ETGES, R. N.; KOSTOVIC, M.; ARAUJO, F. R. |
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Afiliação: |
Ingrid Ieda Fernando de Souza, Universidade Federal de Mato Grosso do Sul - UFMS; Rudielle de Arruda Rodrigues, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; Kláudia Santos Gonçalves Jorge, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; MARCIO ROBERTO SILVA, CNPGL; Walter Lilenbaum, Universidade Federal Fluminense - UFLU/Laboratório de Bacteriologia e Veterinária; Carlos Eugênio Soto Vidal; Rodrigo Nestor Etges; Miladin Kostovic; FLABIO RIBEIRO DE ARAUJO, CNPGC. |
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Título: |
ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
The Journal Veterinary Medical Science, v. 81, n. 1, p. 9-14, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and P=0.12 on Fisher?s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5?89.2%) and 75.5% (95% CI: 62.4?85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03? 100%) and 92.5% specificity (95% CI: 82.1?97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher?s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8?88.6%) and 79.6% (95% CI: 66.4?88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/ MPB83 as the antigen complements the diagnostic coverage provided by CITT and increases the removal of infected animals from herds. MenosBovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and P=0.12 on Fisher?s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5?89.2%) and 75.5% (95% CI: 62.4?85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03? 100%) and 92.5% specificity (95% CI: 82.1?97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher?s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8?88.6%) and 79.6% (95% CI: 66.4?88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/ MPB83 as the antigen complements the diagnostic coverage provided by CITT and inc... Mostrar Tudo |
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Palavras-Chave: |
Comparative intradermal tuberculin test. |
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Thesaurus Nal: |
Bovine tuberculosis; Mycobacterium bovis BCG. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207905/1/ELISA-using-a-recombinant-chimera.pdf
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Marc: |
LEADER 02368naa a2200253 a 4500
001 2117817
005 2019-12-27
008 2019 bl uuuu u00u1 u #d
100 1 $aSOUZA, I. I. F. de
245 $aELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle.$h[electronic resource]
260 $c2019
520 $aBovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and P=0.12 on Fisher?s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5?89.2%) and 75.5% (95% CI: 62.4?85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03? 100%) and 92.5% specificity (95% CI: 82.1?97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher?s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8?88.6%) and 79.6% (95% CI: 66.4?88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/ MPB83 as the antigen complements the diagnostic coverage provided by CITT and increases the removal of infected animals from herds.
650 $aBovine tuberculosis
650 $aMycobacterium bovis BCG
653 $aComparative intradermal tuberculin test
700 1 $aRODRIGUES, R. de A.
700 1 $aJORGE, K. S. G.
700 1 $aSILVA, M. R.
700 1 $aLILENBAUM, W.
700 1 $aVIDAL, C. E. S.
700 1 $aETGES, R. N.
700 1 $aKOSTOVIC, M.
700 1 $aARAUJO, F. R.
773 $tThe Journal Veterinary Medical Science$gv. 81, n. 1, p. 9-14, 2019.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| 1. |  | SOUZA, I. I. F. de; RODRIGUES, R. de A.; JORGE, K. S. G.; SILVA, M. R.; LILENBAUM, W.; VIDAL, C. E. S.; ETGES, R. N.; KOSTOVIC, M.; ARAUJO, F. R. ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle. The Journal Veterinary Medical Science, v. 81, n. 1, p. 9-14, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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