| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
04/11/2019 |
|
Data da última atualização: |
04/11/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SOUZA, A. R. de; YAMIN, M.; GAVA, D.; ZANELLA, J. R. C.; GATTI, M. S. V.; BONAFE, C. S. F.; LIMA NETO, D. F. de. |
|
Afiliação: |
ANCELMO RABELO DE SOUZA, UNICAMP; MARRIAM YAMIN, UNICAMP; DANIELLE GAVA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA; MARIA SILVIA VICCARI GATTI, UNICAMP; CARLOS FRANCISCO SAMPAIO BONAFE, UNICAMP; DANIEL FERREIRA DE LIMA NETO, UNICAMP. |
|
Título: |
Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Virology Journal, v. 16, art. 75, 2019. |
|
DOI: |
10.1186/s12985-019-1165-1 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized. |
|
Palavras-Chave: |
Epitope prediction; Porcine parvovirus; Spot synthesis. |
|
Thesagro: |
Parvovirose; Suinocultura; Virologia. |
|
Thesaurus Nal: |
Epitope mapping; Ungulate protoparvovirus 1. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204209/1/final9080.pdf
|
|
Marc: |
LEADER 02185naa a2200301 a 4500
001 2113864
005 2019-11-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1186/s12985-019-1165-1$2DOI
100 1 $aSOUZA, A. R. de
245 $aPorcine parvovirus VP1/VP2 on a time series epitope mapping$bexploring the effects of high hydrostatic pressure on the immune recognition of antigens.$h[electronic resource]
260 $c2019
520 $aAbstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized.
650 $aEpitope mapping
650 $aUngulate protoparvovirus 1
650 $aParvovirose
650 $aSuinocultura
650 $aVirologia
653 $aEpitope prediction
653 $aPorcine parvovirus
653 $aSpot synthesis
700 1 $aYAMIN, M.
700 1 $aGAVA, D.
700 1 $aZANELLA, J. R. C.
700 1 $aGATTI, M. S. V.
700 1 $aBONAFE, C. S. F.
700 1 $aLIMA NETO, D. F. de
773 $tVirology Journal$gv. 16, art. 75, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | SOUZA, A. R. de; YAMIN, M.; GAVA, D.; ZANELLA, J. R. C.; GATTI, M. S. V.; BONAFE, C. S. F.; LIMA NETO, D. F. de. Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens. Virology Journal, v. 16, art. 75, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
