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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/10/2019 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARRETO, F. Z.; ROSA, J. R. B. F.; BALSALOBRE, T. W. A.; PASTINA, M. M.; SILVA, R. R.; HOFFMANN, H. P.; SOUZA, A. P. de; GARCIA, A. A. F.; CARNEIRO, M. S. |
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Afiliação: |
Fernanda Zatti Barreto, Universidade Federal de São Carlos; João Ricardo Bachega Feijó Rosa, FTS Sementes; Thiago Willian Almeida Balsalobre, Universidade Federal de São Carlos; MARIA MARTA PASTINA, CNPMS; Renato Rodrigues Silva, Universidade Federal de Goiás; Hermann Paulo Hoffmann, Universidade Federal de São Carlos; Anete Pereira de Souza, Universidade Estadual de Campinas; Antonio Augusto Franco Garcia, Escola Superior de Agricultura Luiz de Queiroz; Monalisa Sampaio Carneiro, Universidade Federal de São Carlos. |
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Título: |
A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.). |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Plos ONE, v. 14, n. 7, e0219843, July 2019. |
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DOI: |
10.1371/journal.pone.0219843 |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (65 cM). Genetic diversity analysis showed that, in general, the clustering of accessions within the subpopulations was in accordance with the pedigree information. GWAS performed through a multilocus mixed model revealed 23 MTAs, six, three, seven, four and three for soluble solid content, stalk height, stalk number, stalk weight and cane yield traits, respectively. These MTAs may be validated in other populations to support sugarcane breeding programs with introgression of favorable alleles and marker-assisted selection MenosSugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (... Mostrar Tudo |
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Palavras-Chave: |
Associação ampla do genoma; BPSG; GWAS; Painel Brasileiro de Genótipos de Cana-de-Açúcar. |
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Thesagro: |
Cana de Açúcar; Genoma. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203793/1/Genome-wide.pdf
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Marc: |
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024 7 $a10.1371/journal.pone.0219843$2DOI
100 1 $aBARRETO, F. Z.
245 $aA genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.).$h[electronic resource]
260 $c2019
520 $aSugarcane (Saccharum spp.) has a complex genome with variable ploidy and frequent aneuploidy, which hampers the understanding of phenotype and genotype relations. Despite this complexity, genome-wide association studies (GWAS) may be used to identify favorable alleles for target traits in core collections and then assist breeders in better managing crosses and selecting superior genotypes in breeding populations. Therefore, in the present study, we used a diversity panel of sugarcane, called the Brazilian Panel of Sugarcane Genotypes (BPSG), with the following objectives: (i) estimate, through a mixed model, the adjusted means and genetic parameters of the five yield traits evaluated over two harvest years; (ii) detect population structure, linkage disequilibrium (LD) and genetic diversity using simple sequence repeat (SSR) markers; (iii) perform GWAS analysis to identify marker-trait associations (MTAs); and iv) annotate the sequences giving rise to SSR markers that had fragments associated with target traits to search for putative candidate genes. The phenotypic data analysis showed that the broad-sense heritability values were above 0.48 and 0.49 for the first and second harvests, respectively. The set of 100 SSR markers produced 1,483 fragments, of which 99.5% were polymorphic. These SSR fragments were useful to estimate the most likely number of subpopulations, found to be four, and the LD in BPSG, which was stronger in the first 15 cM and present to a large extension (65 cM). Genetic diversity analysis showed that, in general, the clustering of accessions within the subpopulations was in accordance with the pedigree information. GWAS performed through a multilocus mixed model revealed 23 MTAs, six, three, seven, four and three for soluble solid content, stalk height, stalk number, stalk weight and cane yield traits, respectively. These MTAs may be validated in other populations to support sugarcane breeding programs with introgression of favorable alleles and marker-assisted selection
650 $aCana de Açúcar
650 $aGenoma
653 $aAssociação ampla do genoma
653 $aBPSG
653 $aGWAS
653 $aPainel Brasileiro de Genótipos de Cana-de-Açúcar
700 1 $aROSA, J. R. B. F.
700 1 $aBALSALOBRE, T. W. A.
700 1 $aPASTINA, M. M.
700 1 $aSILVA, R. R.
700 1 $aHOFFMANN, H. P.
700 1 $aSOUZA, A. P. de
700 1 $aGARCIA, A. A. F.
700 1 $aCARNEIRO, M. S.
773 $tPlos ONE$gv. 14, n. 7, e0219843, July 2019.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | BARRETO, F. Z.; ROSA, J. R. B. F.; BALSALOBRE, T. W. A.; PASTINA, M. M.; SILVA, R. R.; HOFFMANN, H. P.; SOUZA, A. P. de; GARCIA, A. A. F.; CARNEIRO, M. S. A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.). Plos ONE, v. 14, n. 7, e0219843, July 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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