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Registro Completo |
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Biblioteca(s): |
Embrapa Acre; Embrapa Rondônia. |
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Data corrente: |
02/09/2019 |
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Data da última atualização: |
11/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZÊVEDO, H. S. F. da S.; RUFINO, P. B.; AZEVEDO, J. M. A. de; SILVA, L. M. da; WADT, L. H. de O.; CAMPOS, T. de. |
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Afiliação: |
LUCIELIO MANOEL DA SILVA, CPAF-AC; LUCIA HELENA DE OLIVEIRA WADT, CPAF-RO; TATIANA DE CAMPOS, CPAF-AC. |
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Título: |
Preservation and maceration of amazon açai leaflet Tissue to obtain genomic DNA. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Bioscience Journal, Uberlândia, v. 35, n. 4, p. 1188-1197, July/Aug. 2019. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/uL (7 days in transport buffer) to 702.00 ng/uL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/uL (30 days in silica gel) to 2,850.00 ng/uL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies. MenosThe objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/uL (7 days in transport buffer) to 702.00 ng/uL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/uL (30 days in silica gel) to 2,850.00 ng/uL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in... Mostrar Tudo |
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Palavras-Chave: |
Açaí-solteiro; Acre; Amazonia Occidental; Amazônia Ocidental; CTAB method; DNA isolation; Embrapa Acre; Extracción; Hojas; Leaflet; Maceración; Rio Branco; Western Amazon. |
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Thesagro: |
Açaí; Campo Experimental; DNA; Extração; Folha; Maceração. |
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Thesaurus Nal: |
Euterpe precatoria; Extraction; Leaves; Maceration. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/201498/1/Azevedo-et-al-2019-PresMacAcaiLeaflet.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209322/1/26962.pdf
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Marc: |
LEADER 03064naa a2200457 a 4500
001 2111807
005 2019-11-11
008 2019 bl uuuu u00u1 u #d
100 1 $aAZÊVEDO, H. S. F. da S.
245 $aPreservation and maceration of amazon açai leaflet Tissue to obtain genomic DNA.$h[electronic resource]
260 $c2019
520 $aThe objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/uL (7 days in transport buffer) to 702.00 ng/uL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/uL (30 days in silica gel) to 2,850.00 ng/uL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.
650 $aEuterpe precatoria
650 $aExtraction
650 $aLeaves
650 $aMaceration
650 $aAçaí
650 $aCampo Experimental
650 $aDNA
650 $aExtração
650 $aFolha
650 $aMaceração
653 $aAçaí-solteiro
653 $aAcre
653 $aAmazonia Occidental
653 $aAmazônia Ocidental
653 $aCTAB method
653 $aDNA isolation
653 $aEmbrapa Acre
653 $aExtracción
653 $aHojas
653 $aLeaflet
653 $aMaceración
653 $aRio Branco
653 $aWestern Amazon
700 1 $aRUFINO, P. B.
700 1 $aAZEVEDO, J. M. A. de
700 1 $aSILVA, L. M. da
700 1 $aWADT, L. H. de O.
700 1 $aCAMPOS, T. de
773 $tBioscience Journal, Uberlândia$gv. 35, n. 4, p. 1188-1197, July/Aug. 2019.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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| 1. |  | AZÊVEDO, H. S. F. da S.; RUFINO, P. B.; AZEVEDO, J. M. A. de; SILVA, L. M. da; WADT, L. H. de O.; CAMPOS, T. de. Preservation and maceration of amazon açai leaflet Tissue to obtain genomic DNA. Bioscience Journal, Uberlândia, v. 35, n. 4, p. 1188-1197, July/Aug. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Acre; Embrapa Rondônia. |
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| Registros recuperados : 1 | |
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