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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
26/08/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, R.; LOPES, J. L.; SOUZA, M. M. de; QUIRINO, B. F.; JUNGMANN, L.; MARINS, L. F. |
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Afiliação: |
Raíza Azevedo, Universidade Federal do Rio Grande do Sul; Jéssika Lawall Lopes, Universidade Federal do Rio Grande do Sul; Manuel Macedo de Souza; BETANIA FERRAZ QUIRINO, CNPAE; LETICIA JUNGMANN CANCADO, CNPAE; Luis Fernando Marins, Universidade Federal do Rio Grande do Sul. |
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Título: |
Synechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant B-glucosidases. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v.12, n. 174, 2019. |
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DOI: |
https://doi.org/10.1186/s13068-019-1505-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose and have low concentration in commercial cocktails used for the production of second-generation (2G) ethanol. Genetic engineering can be used to produce recombinant β-glucosidases, and cyanobacteria may be interesting bioreactors. These photosynthetic microorganisms can be cultured using CO2 emitted from the first-generation ethanol (1G) industry as a carbon source. In addition, vinasse, an effluent of 1G ethanol production, can be used as a source of nitrogen for cyanobacteria growth. Thus, photosynthetic bioreactors cannot only produce cellulases at a lower cost, but also reduce the environmental impact caused by residues of 1G ethanol production. Results: In the present work, we produced a strain of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian soil. For this, the pET system was cloned into cyanobacteria genome. This system uses a dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible promoter. The results showed that the pET system functions efficiently in S. elongatus, once nickel induced T7 RNA polymerase expression which, in turn, induced expression of the gene of the microbial β-glucosidase at high levels when compared with non-induced double transgenic strain. β-glucosidase activity was more than sevenfold higher in the transformed cyanobacteria than in the wild-type strain. Conclusions: The T7 system promotes high expression levels of the cloned gene in S. elongatus, demonstrating that the arrangement in which an exclusive RNA polymerase is used for transcription of heterologous genes may contribute to high-level gene expression in cyanobacteria. This work was the first to demonstrate the use of cyanobacteria for the production of recombinant β-glucosidases. This strategy could be an alternative to reduce the release of 1G ethanol by-products such as CO2 and vinasse, not only contributing to decrease the cost of β-glucosidase production, but also mitigating the environmental impacts of ethanol industrial plants. MenosBackground: The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose and have low concentration in commercial cocktails used for the production of second-generation (2G) ethanol. Genetic engineering can be used to produce recombinant β-glucosidases, and cyanobacteria may be interesting bioreactors. These photosynthetic microorganisms can be cultured using CO2 emitted from the first-generation ethanol (1G) industry as a carbon source. In addition, vinasse, an effluent of 1G ethanol production, can be used as a source of nitrogen for cyanobacteria growth. Thus, photosynthetic bioreactors cannot only produce cellulases at a lower cost, but also reduce the environmental impact caused by residues of 1G ethanol production. Results: In the present work, we produced a strain of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian soil. For this, the pET system was cloned into cyanobacteria genome. This system uses a dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible promoter. The results showed that the pET system functions efficiently in S. elongatus, once nickel induced T7 RNA polymerase expression which, in turn, induced expression of the gene of the microbial β-glucosidase at ... Mostrar Tudo |
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Palavras-Chave: |
PET system. |
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Thesaurus Nal: |
Cellulases; Cyanobacteria; Genetic engineering. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/201297/1/Synechocossus-2019.pdf
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Marc: |
LEADER 03085naa a2200241 a 4500
001 2111615
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13068-019-1505-9$2DOI
100 1 $aAZEVEDO, R.
245 $aSynechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant B-glucosidases.$h[electronic resource]
260 $c2019
520 $aBackground: The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose and have low concentration in commercial cocktails used for the production of second-generation (2G) ethanol. Genetic engineering can be used to produce recombinant β-glucosidases, and cyanobacteria may be interesting bioreactors. These photosynthetic microorganisms can be cultured using CO2 emitted from the first-generation ethanol (1G) industry as a carbon source. In addition, vinasse, an effluent of 1G ethanol production, can be used as a source of nitrogen for cyanobacteria growth. Thus, photosynthetic bioreactors cannot only produce cellulases at a lower cost, but also reduce the environmental impact caused by residues of 1G ethanol production. Results: In the present work, we produced a strain of Synechococcus elongatus capable of expressing high levels of a heterologous β-glucosidase from a microorganism from the Amazonian soil. For this, the pET system was cloned into cyanobacteria genome. This system uses a dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible promoter. The results showed that the pET system functions efficiently in S. elongatus, once nickel induced T7 RNA polymerase expression which, in turn, induced expression of the gene of the microbial β-glucosidase at high levels when compared with non-induced double transgenic strain. β-glucosidase activity was more than sevenfold higher in the transformed cyanobacteria than in the wild-type strain. Conclusions: The T7 system promotes high expression levels of the cloned gene in S. elongatus, demonstrating that the arrangement in which an exclusive RNA polymerase is used for transcription of heterologous genes may contribute to high-level gene expression in cyanobacteria. This work was the first to demonstrate the use of cyanobacteria for the production of recombinant β-glucosidases. This strategy could be an alternative to reduce the release of 1G ethanol by-products such as CO2 and vinasse, not only contributing to decrease the cost of β-glucosidase production, but also mitigating the environmental impacts of ethanol industrial plants.
650 $aCellulases
650 $aCyanobacteria
650 $aGenetic engineering
653 $aPET system
700 1 $aLOPES, J. L.
700 1 $aSOUZA, M. M. de
700 1 $aQUIRINO, B. F.
700 1 $aJUNGMANN, L.
700 1 $aMARINS, L. F.
773 $tBiotechnology for Biofuels$gv.12, n. 174, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
23/01/2020 |
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Data da última atualização: |
18/10/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GUEDES, M. C.; PASTANA, D.; MODENA, E. |
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Afiliação: |
MARCELINO CARNEIRO GUEDES, CPAF-AP; DAYANE PASTANA, CPAF-AP; ÉRICA MODENA, CPAF-AP. |
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Título: |
Dry of Brazil nut trees at Resex Cajari: new evidence that climate anomalies are affecting Amazonian biodiversity. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 47-48, 2019. |
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Idioma: |
Inglês |
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Notas: |
Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
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Conteúdo: |
The Brazil nut (Bertholletia excelsa Bonp.) is among the largest trees in the Amazon. It has big canopy area, and is a deciduous species, indicating high water dependence. In order to investigate Brazil nut trees dry, after strong ?2015 el nino?, we evaluated occurrence and possible explanations of this drought, and relationships with climatic anomalies and nut production. We conduct semi-structured interviews with agroextractivists, and we analyzed leaves and soil near at 15 trees, with and without symptoms of dry. In the years 2015/2016, was registered the strongest El Niño in the Amazon, with an increase of more than 2 °C in the average temperature, reduction of precipitation and prolongation of the dry period. Probably, the water deficit and temperature stress caused the dry (burn) of the leaves and thin branches of the higher Brazil nut trees, observed in the Santa Rosa region, Resex Cajari-Amapá, Eastern Amazonia. The same did not occur in the young trees, in other species and in other regions. There was a drastic fall in fruit production in 2017, and negative correlation of the production with positive anomalies in the Pacific temperature, demonstrating that this species has sensitivity to climate change. Brazil nut trees can recover and sprout new leaves, but the next harvest is it impaired. The nutritional variables did not present significant relationships with trees dry. The hypothesis raised is that this may be related with hydraulic conductance problems, when occurs cavitation and loss of water molecules adhesion, after physiological stress in large trees. MenosThe Brazil nut (Bertholletia excelsa Bonp.) is among the largest trees in the Amazon. It has big canopy area, and is a deciduous species, indicating high water dependence. In order to investigate Brazil nut trees dry, after strong ?2015 el nino?, we evaluated occurrence and possible explanations of this drought, and relationships with climatic anomalies and nut production. We conduct semi-structured interviews with agroextractivists, and we analyzed leaves and soil near at 15 trees, with and without symptoms of dry. In the years 2015/2016, was registered the strongest El Niño in the Amazon, with an increase of more than 2 °C in the average temperature, reduction of precipitation and prolongation of the dry period. Probably, the water deficit and temperature stress caused the dry (burn) of the leaves and thin branches of the higher Brazil nut trees, observed in the Santa Rosa region, Resex Cajari-Amapá, Eastern Amazonia. The same did not occur in the young trees, in other species and in other regions. There was a drastic fall in fruit production in 2017, and negative correlation of the production with positive anomalies in the Pacific temperature, demonstrating that this species has sensitivity to climate change. Brazil nut trees can recover and sprout new leaves, but the next harvest is it impaired. The nutritional variables did not present significant relationships with trees dry. The hypothesis raised is that this may be related with hydraulic conductance problems, when oc... Mostrar Tudo |
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Thesagro: |
Castanha. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209543/1/CPAF-AP-2019-Dry-of-Brazil-nut-at-Resex.pdf
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Marc: |
LEADER 02174nam a2200157 a 4500
001 2119293
005 2022-10-18
008 2019 bl uuuu u00u1 u #d
100 1 $aGUEDES, M. C.
245 $aDry of Brazil nut trees at Resex Cajari$bnew evidence that climate anomalies are affecting Amazonian biodiversity.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 47-48$c2019
500 $aEdição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aThe Brazil nut (Bertholletia excelsa Bonp.) is among the largest trees in the Amazon. It has big canopy area, and is a deciduous species, indicating high water dependence. In order to investigate Brazil nut trees dry, after strong ?2015 el nino?, we evaluated occurrence and possible explanations of this drought, and relationships with climatic anomalies and nut production. We conduct semi-structured interviews with agroextractivists, and we analyzed leaves and soil near at 15 trees, with and without symptoms of dry. In the years 2015/2016, was registered the strongest El Niño in the Amazon, with an increase of more than 2 °C in the average temperature, reduction of precipitation and prolongation of the dry period. Probably, the water deficit and temperature stress caused the dry (burn) of the leaves and thin branches of the higher Brazil nut trees, observed in the Santa Rosa region, Resex Cajari-Amapá, Eastern Amazonia. The same did not occur in the young trees, in other species and in other regions. There was a drastic fall in fruit production in 2017, and negative correlation of the production with positive anomalies in the Pacific temperature, demonstrating that this species has sensitivity to climate change. Brazil nut trees can recover and sprout new leaves, but the next harvest is it impaired. The nutritional variables did not present significant relationships with trees dry. The hypothesis raised is that this may be related with hydraulic conductance problems, when occurs cavitation and loss of water molecules adhesion, after physiological stress in large trees.
650 $aCastanha
700 1 $aPASTANA, D.
700 1 $aMODENA, E.
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