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Registro Completo |
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
Data corrente: |
18/02/2019 |
Data da última atualização: |
19/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GIUSTINA, L. D.; CABRAL, J. C.; BALDONI, A. B.; NEVES, L. G. |
Afiliação: |
LUANA DELLA GIUSTINA, ESTUDANTE DE PhD, UFMT, CUIABA; JULIANE COSTA CABRAL, MESTRANDA DA UNEMAT, ALTA FLORESTA; AISY BOTEGA BALDONI TARDIN, CPAMT; LEONARDA GRILLO NEVES, UNEMAT, CACERES, MT. |
Título: |
Protocol adaptation for brazil nut (Bertholletia excelsa Bonpl) DNA extraction. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
International Journal of Current Research, v. 10, n. 02, p. 65270-65275, 2018. |
ISSN: |
0975-833X |
Idioma: |
Inglês |
Conteúdo: |
Brazil nut (Bertholletia excelsa Bonpl.) is an Amazonian Forest species, which is acknowledged for the commercialization of its almonds. It is an endangered species, fact that reinforces the need of conducting conservation studies. Molecular markers are tools that may be used in conservation studies and the primordial stage preceding the use of molecular markers lies on DNA extraction. A well-designed protocol allows isolating DNA in sufficient amount and quality to conduct molecular studies. Thus, the aim of the current study is to adapt a total DNA extraction protocol to be applied to Brazil nut plants. Tests were conducted at different CTAB (2% and 4%) and β-Mercaptoethanol (0.2%, 0.8% and 1.4%) concentrations; washes using chloroform: isoamyl alcohol (CIA) were performed (one or two washes). The extraction products were confirmed in 0.8% agarose gel and quantified in spectrophotometer (in ng μL-1). Plant tissue (vascular cambium and leaf) was collected from 30 Brazil nut individuals in order to confirm the efficiency of the protocol adapted to the species. Samples were subjected to PCR amplification using 10 microsatellite markers. CTAB and β-Mercaptoethanol at concentrations 4% and 0.2%, respectively, along with one wash using CIA, showed the best results when the quality of the extracted material was assessed through its absorbance ratios. The vascular cambium oxidized more easily than the leaf during the DNA isolation process. Thus, it is essential taking some precautions at the time to handle it during collection and storage to assure process efficiency. MenosBrazil nut (Bertholletia excelsa Bonpl.) is an Amazonian Forest species, which is acknowledged for the commercialization of its almonds. It is an endangered species, fact that reinforces the need of conducting conservation studies. Molecular markers are tools that may be used in conservation studies and the primordial stage preceding the use of molecular markers lies on DNA extraction. A well-designed protocol allows isolating DNA in sufficient amount and quality to conduct molecular studies. Thus, the aim of the current study is to adapt a total DNA extraction protocol to be applied to Brazil nut plants. Tests were conducted at different CTAB (2% and 4%) and β-Mercaptoethanol (0.2%, 0.8% and 1.4%) concentrations; washes using chloroform: isoamyl alcohol (CIA) were performed (one or two washes). The extraction products were confirmed in 0.8% agarose gel and quantified in spectrophotometer (in ng μL-1). Plant tissue (vascular cambium and leaf) was collected from 30 Brazil nut individuals in order to confirm the efficiency of the protocol adapted to the species. Samples were subjected to PCR amplification using 10 microsatellite markers. CTAB and β-Mercaptoethanol at concentrations 4% and 0.2%, respectively, along with one wash using CIA, showed the best results when the quality of the extracted material was assessed through its absorbance ratios. The vascular cambium oxidized more easily than the leaf during the DNA isolation process. Thus, it is essential taki... Mostrar Tudo |
Palavras-Chave: |
B-Mercaptoethanol; CTAB; Mato Grosso; Molecular analyze; Molecular marker; Sinop; SSR. |
Thesagro: |
Bertholletia Excelsa; DNA; Marcador Molecular. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192934/1/2018-cpamt-aisy-baldoni-protocol-adaptation-brazil-bertholletia-dna-extraction.pdf
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Marc: |
LEADER 02409naa a2200289 a 4500 001 2106148 005 2019-02-19 008 2018 bl uuuu u00u1 u #d 022 $a0975-833X 100 1 $aGIUSTINA, L. D. 245 $aProtocol adaptation for brazil nut (Bertholletia excelsa Bonpl) DNA extraction.$h[electronic resource] 260 $c2018 520 $aBrazil nut (Bertholletia excelsa Bonpl.) is an Amazonian Forest species, which is acknowledged for the commercialization of its almonds. It is an endangered species, fact that reinforces the need of conducting conservation studies. Molecular markers are tools that may be used in conservation studies and the primordial stage preceding the use of molecular markers lies on DNA extraction. A well-designed protocol allows isolating DNA in sufficient amount and quality to conduct molecular studies. Thus, the aim of the current study is to adapt a total DNA extraction protocol to be applied to Brazil nut plants. Tests were conducted at different CTAB (2% and 4%) and β-Mercaptoethanol (0.2%, 0.8% and 1.4%) concentrations; washes using chloroform: isoamyl alcohol (CIA) were performed (one or two washes). The extraction products were confirmed in 0.8% agarose gel and quantified in spectrophotometer (in ng μL-1). Plant tissue (vascular cambium and leaf) was collected from 30 Brazil nut individuals in order to confirm the efficiency of the protocol adapted to the species. Samples were subjected to PCR amplification using 10 microsatellite markers. CTAB and β-Mercaptoethanol at concentrations 4% and 0.2%, respectively, along with one wash using CIA, showed the best results when the quality of the extracted material was assessed through its absorbance ratios. The vascular cambium oxidized more easily than the leaf during the DNA isolation process. Thus, it is essential taking some precautions at the time to handle it during collection and storage to assure process efficiency. 650 $aBertholletia Excelsa 650 $aDNA 650 $aMarcador Molecular 653 $aB-Mercaptoethanol 653 $aCTAB 653 $aMato Grosso 653 $aMolecular analyze 653 $aMolecular marker 653 $aSinop 653 $aSSR 700 1 $aCABRAL, J. C. 700 1 $aBALDONI, A. B. 700 1 $aNEVES, L. G. 773 $tInternational Journal of Current Research$gv. 10, n. 02, p. 65270-65275, 2018.
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Embrapa Agrossilvipastoril (CPAMT) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
01/10/2014 |
Data da última atualização: |
18/04/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SANTOS, J. S. dos; LOPES, P. R. C.; OLIVEIRA, I. V. de M.; SILVA-MATOS, R. R. S. da; DANTAS, M. R. |
Afiliação: |
JACQUELINE SOUZA DOS SANTOS; PAULO ROBERTO COELHO LOPES, CPATSA; INEZ VILAR DE MORAIS OLIVEIRA; RAISSA RACHEL SALUSTRIANO DA SILVA-MATOS; MAYARA RAMALHO DANTAS. |
Título: |
Caracterização fenológia da pereira Stchimidt cultivada no Vale do São Francisco. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 23., 2014, Cuiabá. Fruticultura: oportunidades e desafios para o Brasil. Cuiabá: SBF, 2014. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Conteúdo: |
O presente trabalho foi desenvolvido com o objetivo de caracterizar os estádios fenológicos da pereira cv. Schimidt e verificar a frutificação efetiva na região do Submédio do Vale do São Francisco, em Petrolina, PE. |
Palavras-Chave: |
Cultivo; Vale do São Francisco. |
Thesagro: |
Fenologia; Fruta; Pêra. |
Thesaurus NAL: |
Fruits. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/109368/1/Paulo-Roberto-8.pdf
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Marc: |
LEADER 00972nam a2200241 a 4500 001 1996258 005 2023-04-18 008 2014 bl uuuu u00u1 u #d 100 1 $aSANTOS, J. S. dos 245 $aCaracterização fenológia da pereira Stchimidt cultivada no Vale do São Francisco. 260 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 23., 2014, Cuiabá. Fruticultura: oportunidades e desafios para o Brasil. Cuiabá: SBF$c2014 300 $c1 CD-ROM. 520 $aO presente trabalho foi desenvolvido com o objetivo de caracterizar os estádios fenológicos da pereira cv. Schimidt e verificar a frutificação efetiva na região do Submédio do Vale do São Francisco, em Petrolina, PE. 650 $aFruits 650 $aFenologia 650 $aFruta 650 $aPêra 653 $aCultivo 653 $aVale do São Francisco 700 1 $aLOPES, P. R. C. 700 1 $aOLIVEIRA, I. V. de M. 700 1 $aSILVA-MATOS, R. R. S. da 700 1 $aDANTAS, M. R.
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