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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
05/02/2019 |
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Data da última atualização: |
21/01/2020 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SOUZA, M. M.; ZERLOTINI NETO, A.; NICIURA, S. C. M.; ROCHA, M. I. P.; DINIZ, W. J.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; LIMA, A. O.; AFONSO, J.; CESAR, A. S. M; COUTINHO, L. L.; REGITANO, L. C. de A. |
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Afiliação: |
M. M. SOUZA, CPPSE; ADHEMAR ZERLOTINI NETO, CNPTIA; SIMONE CRISTINA MEO NICIURA, CPPSE; MARINA IBELLI PEREIRA ROCHA, UFSCar; WELLISON JARLES DINIZ, UFSCar; POLYANA CRISTINE TIZIOTO, Esalq/USP; PRISCILA SILVA NEUBERN DE OLIVEIRA, Bolsista/CPPSE; A.O. LIMA, UFSCar; J. AFONSO, UFSCar; A. S. M. CESAR, CNPTIA; L. L. COUTINHO, CNPTIA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Genome-wide distribution of allele-specific expression in Nelore steers muscle. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland. Proceedings... [S.l.: s.n.], 2018. |
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Páginas: |
5 p. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: A. Zerlotini, L. C. A. Regitano. WCGALP 2018. |
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Conteúdo: |
Summary. The difference between the allelic expression of a gene is known as allele-specific expression (ASE), and it is a common event in mammal?s transcriptome. ASE is named genomic imprinting when guided by epigenetics mechanisms regarding the allele parental origin. Although these events are an important sources of phenotypic variation, knowledge thereof regarding cattle and effects on economically important traits are still incipient. Therefore, analysis and characterization of ASE profile in the muscle of adult Nelore animals were performed, and besides that, the ASE pattern and gene imprinting occurrence were checked in genes previously associated with meat tenderness. For this, RNA sequencing data of 146 Nelore steer muscles were used, and these animals were also genotyped for approximately 770,000 SNPs using Illumina BovineHD BeadChip. For each animal, a diploid genome was built based on their genotypes, and afterward, RNA-Seq reads were mapped to this genome. After counting the reads considering each SNP allele, a binomial test was applied to identify whether the allele contribution to total expression significantly diverged from 50%, followed by false discovery rate (FDR) test (0.05). Among 519 analyzed SNPs, 402 showed at least one sample with ASE, and they were spread among all autosomal chromosomes. SNPs showing ASE in more than five samples were tested regarding ASE extension, and most presented an incomplete pattern. Four ASE genes were previously identified in a Genome-Wide Association Study (GWAS) and five were found differentially expressed between extreme phenotype animals for meat tenderness. Therefore, the results will complement the bovine imprinted genes database, further they can provide new information to genetic effects prediction in animal breeding increasing the accuracy of meat tenderness selection. MenosSummary. The difference between the allelic expression of a gene is known as allele-specific expression (ASE), and it is a common event in mammal?s transcriptome. ASE is named genomic imprinting when guided by epigenetics mechanisms regarding the allele parental origin. Although these events are an important sources of phenotypic variation, knowledge thereof regarding cattle and effects on economically important traits are still incipient. Therefore, analysis and characterization of ASE profile in the muscle of adult Nelore animals were performed, and besides that, the ASE pattern and gene imprinting occurrence were checked in genes previously associated with meat tenderness. For this, RNA sequencing data of 146 Nelore steer muscles were used, and these animals were also genotyped for approximately 770,000 SNPs using Illumina BovineHD BeadChip. For each animal, a diploid genome was built based on their genotypes, and afterward, RNA-Seq reads were mapped to this genome. After counting the reads considering each SNP allele, a binomial test was applied to identify whether the allele contribution to total expression significantly diverged from 50%, followed by false discovery rate (FDR) test (0.05). Among 519 analyzed SNPs, 402 showed at least one sample with ASE, and they were spread among all autosomal chromosomes. SNPs showing ASE in more than five samples were tested regarding ASE extension, and most presented an incomplete pattern. Four ASE genes were previously identified ... Mostrar Tudo |
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Palavras-Chave: |
Allele-specific expression; Expressão gênica; Sequenciamento; Sequencing; Transcriptoma. |
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Thesagro: |
Gado Nelore; Melhoramento Genético Animal; Polimorfismo Genético. |
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Thesaurus Nal: |
Gene expression; Transcriptome. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03075nam a2200385 a 4500
001 2105522
005 2020-01-21
008 2018 bl uuuu u01u1 u #d
100 1 $aSOUZA, M. M.
245 $aGenome-wide distribution of allele-specific expression in Nelore steers muscle.$h[electronic resource]
260 $aIn: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland. Proceedings... [S.l.: s.n.]$c2018
300 $a5 p.
500 $aNa publicação: A. Zerlotini, L. C. A. Regitano. WCGALP 2018.
520 $aSummary. The difference between the allelic expression of a gene is known as allele-specific expression (ASE), and it is a common event in mammal?s transcriptome. ASE is named genomic imprinting when guided by epigenetics mechanisms regarding the allele parental origin. Although these events are an important sources of phenotypic variation, knowledge thereof regarding cattle and effects on economically important traits are still incipient. Therefore, analysis and characterization of ASE profile in the muscle of adult Nelore animals were performed, and besides that, the ASE pattern and gene imprinting occurrence were checked in genes previously associated with meat tenderness. For this, RNA sequencing data of 146 Nelore steer muscles were used, and these animals were also genotyped for approximately 770,000 SNPs using Illumina BovineHD BeadChip. For each animal, a diploid genome was built based on their genotypes, and afterward, RNA-Seq reads were mapped to this genome. After counting the reads considering each SNP allele, a binomial test was applied to identify whether the allele contribution to total expression significantly diverged from 50%, followed by false discovery rate (FDR) test (0.05). Among 519 analyzed SNPs, 402 showed at least one sample with ASE, and they were spread among all autosomal chromosomes. SNPs showing ASE in more than five samples were tested regarding ASE extension, and most presented an incomplete pattern. Four ASE genes were previously identified in a Genome-Wide Association Study (GWAS) and five were found differentially expressed between extreme phenotype animals for meat tenderness. Therefore, the results will complement the bovine imprinted genes database, further they can provide new information to genetic effects prediction in animal breeding increasing the accuracy of meat tenderness selection.
650 $aGene expression
650 $aTranscriptome
650 $aGado Nelore
650 $aMelhoramento Genético Animal
650 $aPolimorfismo Genético
653 $aAllele-specific expression
653 $aExpressão gênica
653 $aSequenciamento
653 $aSequencing
653 $aTranscriptoma
700 1 $aZERLOTINI NETO, A.
700 1 $aNICIURA, S. C. M.
700 1 $aROCHA, M. I. P.
700 1 $aDINIZ, W. J.
700 1 $aTIZIOTO, P. C.
700 1 $aOLIVEIRA, P. S. N.
700 1 $aLIMA, A. O.
700 1 $aAFONSO, J.
700 1 $aCESAR, A. S. M
700 1 $aCOUTINHO, L. L.
700 1 $aREGITANO, L. C. de A.
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Embrapa Agricultura Digital (CNPTIA) |
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| 1. |  | SOUZA, M. M.; ZERLOTINI NETO, A.; NICIURA, S. C. M.; ROCHA, M. I. P.; DINIZ, W. J.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; LIMA, A. O.; AFONSO, J.; CESAR, A. S. M; COUTINHO, L. L.; REGITANO, L. C. de A. Genome-wide distribution of allele-specific expression in Nelore steers muscle. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland. Proceedings... [S.l.: s.n.], 2018. 5 p. Na publicação: A. Zerlotini, L. C. A. Regitano. WCGALP 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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