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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Indian Journal of Science and Technology, v. 11, n. 25, 2018. |
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Páginas: |
9 p. |
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DOI: |
10.17485/ijst%2F2018%2Fv11i25%2F128251 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP. |
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Palavras-Chave: |
Embryonic Development; Epigenetic Modification; Nuclear Transfer. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189328/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Effect.pdf
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Marc: |
LEADER 01943naa a2200205 a 4500
001 2102530
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.17485/ijst%2F2018%2Fv11i25%2F128251$2DOI
100 1 $aOTERO, R.
245 $aEffect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.$h[electronic resource]
260 $c2018
300 $a9 p.
520 $aAbstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.
653 $aEmbryonic Development
653 $aEpigenetic Modification
653 $aNuclear Transfer
700 1 $aHERNÁNDEZ, D.
700 1 $aCAMARGO, L. S. de A.
773 $tIndian Journal of Science and Technology$gv. 11, n. 25, 2018.
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Embrapa Gado de Leite (CNPGL) |
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