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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
03/12/2018 |
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Data da última atualização: |
22/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VENTURELLI, G. L.; SILVA, K. J. da; TREML, D.; NAVAS, P. B.; VARGAS, M. O.; BISCHOFF, J. L.; FARIA, J. C. de; ARISI, A. C. M. |
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Afiliação: |
GUSTAVO LUIZ VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; KELLY JUSTIN DA SILVA, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; PAOLA BEATRIZ NAVAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; MARYELLA OSÓRIO VARGAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; JOANA LAURA BISCHOFF, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; JOSIAS CORREA DE FARIA, CNPAF; ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC. |
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Título: |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Food Bioscience, v. 26, p. 145-151, Dec. 2018. |
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ISSN: |
2212-4292 |
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DOI: |
10.1016/j.fbio.2018.10.007 |
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Idioma: |
Inglês |
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Conteúdo: |
The geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization. |
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Palavras-Chave: |
Certified reference material; EMB-PV051-1-event. |
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Thesagro: |
Engenharia Genética; Feijão; Organismo Transgênico; Phaseolus Vulgaris; Plasmídeo. |
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Thesaurus Nal: |
Beans; Genetically modified organisms; Plasmids; Quantitative polymerase chain reaction. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187555/1/CNPAF-2018-fb.pdf
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Marc: |
LEADER 02151naa a2200361 a 4500
001 2100557
005 2019-01-22
008 2018 bl uuuu u00u1 u #d
022 $a2212-4292
024 7 $a10.1016/j.fbio.2018.10.007$2DOI
100 1 $aVENTURELLI, G. L.
245 $aNew plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR.$h[electronic resource]
260 $c2018
520 $aThe geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization.
650 $aBeans
650 $aGenetically modified organisms
650 $aPlasmids
650 $aQuantitative polymerase chain reaction
650 $aEngenharia Genética
650 $aFeijão
650 $aOrganismo Transgênico
650 $aPhaseolus Vulgaris
650 $aPlasmídeo
653 $aCertified reference material
653 $aEMB-PV051-1-event
700 1 $aSILVA, K. J. da
700 1 $aTREML, D.
700 1 $aNAVAS, P. B.
700 1 $aVARGAS, M. O.
700 1 $aBISCHOFF, J. L.
700 1 $aFARIA, J. C. de
700 1 $aARISI, A. C. M.
773 $tFood Bioscience$gv. 26, p. 145-151, Dec. 2018.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| 1. |  | VENTURELLI, G. L.; SILVA, K. J. da; TREML, D.; NAVAS, P. B.; VARGAS, M. O.; BISCHOFF, J. L.; FARIA, J. C. de; ARISI, A. C. M. New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. Food Bioscience, v. 26, p. 145-151, Dec. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 1 | |
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