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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
23/11/2018 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PINHEIRO, D. H.; VÉLEZ, A. M.; FISHILEVICH, E.; WANG, H.; CARNEIRO, N. P.; VALENCIA-JIMÉNEZ, A.; VALICENTE, F. H.; NARVA, E. K.; SIEGFRIED, B. D. |
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Afiliação: |
Daniele H. Pinheiro, Universidade Federal de Lavras; Ana M. Vélez, University of Lincoln; Elane Fishilevich, University of Lincoln; Haichuan Wang, University of Lincoln; NEWTON PORTILHO CARNEIRO, CNPMS; Arnubio Valencia-Jiménez, Universidad de Caldas, Colombia; FERNANDO HERCOS VALICENTE, CNPMS; Kenneth E. Narva, DowDuPont; Blair D. Siegfried, University of Florida. |
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Título: |
Clathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeCont. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, v. 13, n. 8, e0201849, Aug. 2018. |
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DOI: |
10.1371/journal.pone.0201849 |
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Idioma: |
Inglês |
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Conteúdo: |
The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H+ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, and AP50 led to a significant decrease in the effects of laccase2 dsRNA reporter, indicating that these genes are involved in RNAi response. However, the knockdown of either Arf72A or Rab7 did not suppress the response to laccase2 dsRNA. The silencing of the silC gene did not lead to a significant reduction in mortality or increase in the expression of V-ATPase A reporter. While the silencing of the silA gene significantly decreased insect mortality, significant changes in V-ATPase A expression were not detected. These results suggest that clathrin-dependent endocytosis is a biological mechanism that plays an important role during RNAi response in WCR adults. The fact that no definitive support for the roles of silA or silC in RNAi response was obtained support the idea that RNAi response varies greatly in different insect species, demanding additional studies focused on elucidating their involvement in this mechanism. |
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Palavras-Chave: |
Clatrina; Endocitose. |
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Thesagro: |
Gene; Inseto; Proteína. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186961/1/Clathrin-dependent.pdf
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Marc: |
LEADER 02340naa a2200289 a 4500
001 2099972
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008 2018 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0201849$2DOI
100 1 $aPINHEIRO, D. H.
245 $aClathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeCont.$h[electronic resource]
260 $c2018
520 $aThe cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H+ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, and AP50 led to a significant decrease in the effects of laccase2 dsRNA reporter, indicating that these genes are involved in RNAi response. However, the knockdown of either Arf72A or Rab7 did not suppress the response to laccase2 dsRNA. The silencing of the silC gene did not lead to a significant reduction in mortality or increase in the expression of V-ATPase A reporter. While the silencing of the silA gene significantly decreased insect mortality, significant changes in V-ATPase A expression were not detected. These results suggest that clathrin-dependent endocytosis is a biological mechanism that plays an important role during RNAi response in WCR adults. The fact that no definitive support for the roles of silA or silC in RNAi response was obtained support the idea that RNAi response varies greatly in different insect species, demanding additional studies focused on elucidating their involvement in this mechanism.
650 $aGene
650 $aInseto
650 $aProteína
653 $aClatrina
653 $aEndocitose
700 1 $aVÉLEZ, A. M.
700 1 $aFISHILEVICH, E.
700 1 $aWANG, H.
700 1 $aCARNEIRO, N. P.
700 1 $aVALENCIA-JIMÉNEZ, A.
700 1 $aVALICENTE, F. H.
700 1 $aNARVA, E. K.
700 1 $aSIEGFRIED, B. D.
773 $tPlos One$gv. 13, n. 8, e0201849, Aug. 2018.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
24/02/2022 |
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Data da última atualização: |
24/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ZORTÉA, K. É. M.; ROSSI, A. A. B.; TIAGO, A. V.; CARDOSO, E. dos S.; PINTO, J. M. A.; HOOGERHEIDE, E. S. S. |
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Afiliação: |
KELLI ÉVELIN MÜLLER ZORTÉA, UNEMAT, Alta Floresta-MT; ANA APARECIDA BANDINI ROSSI, UNEMAT, Alta Floresta-MT; AUANA VICENTE TIAGO, UNEMAT, Alta Floresta-MT; ELISA DOS SANTOS CARDOSO, UNEMAT, Alta Floresta-MT; JOYCE MENDES ANDRADE PINTO, CPAMT; EULALIA SOLER SOBREIRA HOOGERHEIDE, CPAMT. |
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Título: |
Spondias mombin (Anarcadiaceae): molecular characterization and conservation. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista de Biología Tropical, v. 69, n. 3, p. 1023-1036, 2021. |
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ISSN: |
2215-2075 |
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DOI: |
https://doi.org/10.15517/rbt.v69i3.45810 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Introduction: The fruit of the yellow mombin (Spondias mombin L.) is notable due to its sensory and functional qualities. However, there is little knowledge regarding the genetic diversity of this species, and this would aid the implantation of the cultivation of the fruit as a crop, since current production is based on extractivism. Objective: Evaluate the diversity and genetic structure of natural populations of S. mombin in the state of Mato Grosso, Brazil, through microsatellite molecular markers in order to assist in the implementation of conservation strategies and the collection of genetic resources. Methods: A total of 139 S. mombin individuals were sampled in ten natural populations. PCR amplifications were performed with seven fluorescence-marked microsatellite primers. Genetic diversity was evaluated by the number of alleles, expected (He) and observed heterozygosity (Ho), polymorphic information content (PIC), fixation index (?), rare and exclusive alleles. The genetic structure was evaluated using AMOVA, UPGMA dendrogram and Bayesian statistical analysis. Results: 46 alleles were amplified, which had an average of 6.6 alleles per locus. He was higher than Ho and f was positive, indicating the presence of inbreeding. The PIC ranged from 0.048 to 0.700, and only two loci were poorly informative. We found 27 rare alleles and 16 unique alleles. The largest component of variation was intrapopulational (90.64 %). The estimated gene flow was 1.99, which indicates that there is no genetic isolation between populations, and justifies the FST value (0.0963). The ten populations were grouped into two groups, and two populations constituted an isolated group. The Mantel test demonstrated that the genetic structure is not related to the geographic distance between populations. Conclusion: There is genetic diversity in the populations of S. mombin, which must be conserved in situ or ex situ, due to the diversity they present and because they are promising sources for collection of germplasm. MenosAbstract: Introduction: The fruit of the yellow mombin (Spondias mombin L.) is notable due to its sensory and functional qualities. However, there is little knowledge regarding the genetic diversity of this species, and this would aid the implantation of the cultivation of the fruit as a crop, since current production is based on extractivism. Objective: Evaluate the diversity and genetic structure of natural populations of S. mombin in the state of Mato Grosso, Brazil, through microsatellite molecular markers in order to assist in the implementation of conservation strategies and the collection of genetic resources. Methods: A total of 139 S. mombin individuals were sampled in ten natural populations. PCR amplifications were performed with seven fluorescence-marked microsatellite primers. Genetic diversity was evaluated by the number of alleles, expected (He) and observed heterozygosity (Ho), polymorphic information content (PIC), fixation index (?), rare and exclusive alleles. The genetic structure was evaluated using AMOVA, UPGMA dendrogram and Bayesian statistical analysis. Results: 46 alleles were amplified, which had an average of 6.6 alleles per locus. He was higher than Ho and f was positive, indicating the presence of inbreeding. The PIC ranged from 0.048 to 0.700, and only two loci were poorly informative. We found 27 rare alleles and 16 unique alleles. The largest component of variation was intrapopulational (90.64 %). The estimated gene flow was 1.99, which indic... Mostrar Tudo |
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Thesaurus NAL: |
Conservation programs; Genetic resources; Microsatellite repeats; Variability; Yellow mombins. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231920/1/2021-cpamt-essh-spondias-mombin-anarcadiaceae-molecular-characterization-conservation.pdf
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Marc: |
LEADER 02851naa a2200265 a 4500
001 2140386
005 2022-02-24
008 2021 bl uuuu u00u1 u #d
022 $a2215-2075
024 7 $ahttps://doi.org/10.15517/rbt.v69i3.45810$2DOI
100 1 $aZORTÉA, K. É. M.
245 $aSpondias mombin (Anarcadiaceae)$bmolecular characterization and conservation.$h[electronic resource]
260 $c2021
520 $aAbstract: Introduction: The fruit of the yellow mombin (Spondias mombin L.) is notable due to its sensory and functional qualities. However, there is little knowledge regarding the genetic diversity of this species, and this would aid the implantation of the cultivation of the fruit as a crop, since current production is based on extractivism. Objective: Evaluate the diversity and genetic structure of natural populations of S. mombin in the state of Mato Grosso, Brazil, through microsatellite molecular markers in order to assist in the implementation of conservation strategies and the collection of genetic resources. Methods: A total of 139 S. mombin individuals were sampled in ten natural populations. PCR amplifications were performed with seven fluorescence-marked microsatellite primers. Genetic diversity was evaluated by the number of alleles, expected (He) and observed heterozygosity (Ho), polymorphic information content (PIC), fixation index (?), rare and exclusive alleles. The genetic structure was evaluated using AMOVA, UPGMA dendrogram and Bayesian statistical analysis. Results: 46 alleles were amplified, which had an average of 6.6 alleles per locus. He was higher than Ho and f was positive, indicating the presence of inbreeding. The PIC ranged from 0.048 to 0.700, and only two loci were poorly informative. We found 27 rare alleles and 16 unique alleles. The largest component of variation was intrapopulational (90.64 %). The estimated gene flow was 1.99, which indicates that there is no genetic isolation between populations, and justifies the FST value (0.0963). The ten populations were grouped into two groups, and two populations constituted an isolated group. The Mantel test demonstrated that the genetic structure is not related to the geographic distance between populations. Conclusion: There is genetic diversity in the populations of S. mombin, which must be conserved in situ or ex situ, due to the diversity they present and because they are promising sources for collection of germplasm.
650 $aConservation programs
650 $aGenetic resources
650 $aMicrosatellite repeats
650 $aVariability
650 $aYellow mombins
700 1 $aROSSI, A. A. B.
700 1 $aTIAGO, A. V.
700 1 $aCARDOSO, E. dos S.
700 1 $aPINTO, J. M. A.
700 1 $aHOOGERHEIDE, E. S. S.
773 $tRevista de Biología Tropical$gv. 69, n. 3, p. 1023-1036, 2021.
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Embrapa Agrossilvipastoril (CPAMT) |
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