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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/10/2018 |
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Data da última atualização: |
24/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, C. S. F.; SILVEIRA, L. C. P.; PAULA, D. P.; ANDOW, D. A.; MENDES, S. M. |
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Afiliação: |
Camila S. F. Souza, Universidade Federal de Lavras; Luís C. P. Silveira, Universidade Federal de Lavras; DEBORA PIRES PAULA, Cenargen; David A. Andow, University of Minnesota; SIMONE MARTINS MENDES, CNPMS. |
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Título: |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, v. 13, n. 9, e0203791, Sept. 2018. |
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DOI: |
10.1371 /journal.pone. 0203791 |
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Idioma: |
Inglês |
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Conteúdo: |
The intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. Cry toxins are proteins naturally produced by the soil bacterium Bacillus thuringiensis (Bt) and its genes have been expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae of a major maize pest, Spodoptera frugiperda, with resistance to Cry1F, could transfer Cry1F from a genetically engineered maize variety to their offspring. Resistant 10day-old larvae that fed on Cry1F Bt maize until pupation were sexed and pair-mated to produce eggs. Using ELISA we found that Cry1F was transferred to offspring (1.47–4.42 ng Cry1F/10 eggs), a toxin concentration about 28–83 times less than that detected in Cry1F Bt maize leaves. This occurred when only one or both sexes were exposed, and more was transferred when both parents were exposed, with transitory detection in the first five egg masses. This work is an unprecedented demonstration that a non-aposematic, but resistant, species can transfer Cry1F to their offspring when exposed to Bt host plant leaves as immatures. |
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Palavras-Chave: |
Cry toxins; Lagarta-do-cartucho; Maize pest; Soil bacterium. |
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Thesagro: |
Bacillus Thuringiensis; Controle Biológico; Praga de Planta; Proteína. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186962/1/Transfer-Cry.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184056/1/journal.pone.0203791.pdf
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Marc: |
LEADER 01964naa a2200277 a 4500
001 2099951
005 2018-11-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1371 /journal.pone. 0203791$2DOI
100 1 $aSOUZA, C. S. F.
245 $aTransfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda.$h[electronic resource]
260 $c2018
520 $aThe intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. Cry toxins are proteins naturally produced by the soil bacterium Bacillus thuringiensis (Bt) and its genes have been expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae of a major maize pest, Spodoptera frugiperda, with resistance to Cry1F, could transfer Cry1F from a genetically engineered maize variety to their offspring. Resistant 10day-old larvae that fed on Cry1F Bt maize until pupation were sexed and pair-mated to produce eggs. Using ELISA we found that Cry1F was transferred to offspring (1.47–4.42 ng Cry1F/10 eggs), a toxin concentration about 28–83 times less than that detected in Cry1F Bt maize leaves. This occurred when only one or both sexes were exposed, and more was transferred when both parents were exposed, with transitory detection in the first five egg masses. This work is an unprecedented demonstration that a non-aposematic, but resistant, species can transfer Cry1F to their offspring when exposed to Bt host plant leaves as immatures.
650 $aBacillus Thuringiensis
650 $aControle Biológico
650 $aPraga de Planta
650 $aProteína
653 $aCry toxins
653 $aLagarta-do-cartucho
653 $aMaize pest
653 $aSoil bacterium
700 1 $aSILVEIRA, L. C. P.
700 1 $aPAULA, D. P.
700 1 $aANDOW, D. A.
700 1 $aMENDES, S. M.
773 $tPlos One$gv. 13, n. 9, e0203791, Sept. 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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