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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/10/2018 |
Data da última atualização: |
16/10/2018 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
MONDIN, M.; SEREJO, J. A. dos S.; BERTÃO, M. R.; LABORDA, P.; PIZZAIA, D.; AGUIAR-PERECIN, M. L. R. |
Afiliação: |
MATEUS MONDIN, ESALQ; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; MÔNICA R. BERTÃO, ESALQ; PRIANDA LABORDA, UNICAMP; DANIEL PIZZAIA, ESALQ; MARGARIDA L. R. AGUIAR-PERECIN, ESALQ. |
Título: |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with kys standard line. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: TOP 10 Contributions on Plant Biology. Local: Telangana, India: Avid Science, 2018. |
Idioma: |
Inglês |
Conteúdo: |
Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise q MenosMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes.... Mostrar Tudo |
Palavras-Chave: |
Heterochromatic Knobs. |
Thesagro: |
DNA; Milho. |
Thesaurus Nal: |
Centromeres; Chromosomes; Satellite DNA. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184478/1/Karyotype-Variability-in-Tropical-Maize-Sister-Inbred-Lines-and-Hybrids-Compared-with-KYS-Standard-Line.pdf
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Marc: |
LEADER 02643naa a2200253 a 4500 001 2097537 005 2018-10-16 008 2018 bl uuuu u00u1 u #d 100 1 $aMONDIN, M. 245 $aKaryotype variability in tropical maize sister inbred lines and hybrids compared with kys standard line.$h[electronic resource] 260 $c2018 520 $aMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise q 650 $aCentromeres 650 $aChromosomes 650 $aSatellite DNA 650 $aDNA 650 $aMilho 653 $aHeterochromatic Knobs 700 1 $aSEREJO, J. A. dos S. 700 1 $aBERTÃO, M. R. 700 1 $aLABORDA, P. 700 1 $aPIZZAIA, D. 700 1 $aAGUIAR-PERECIN, M. L. R. 773 $tIn: TOP 10 Contributions on Plant Biology. Local: Telangana, India: Avid Science, 2018.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
28/01/2016 |
Data da última atualização: |
28/01/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SCOTTI-CAMPOS, P.; PAIS, I. P.; LIDON, F. C.; MARTINS, L. A.; TOMAZ, M. A.; SEMEDO, J. N.; RODRIGUES, A. P.; FORTUNATO, A. S.; PALOS, I.; BATISTA-SANTOS, P.; LEITÃO, A. E.; LOPES, E.; SIMÕES-COSTA, M. C.; GOULÃO, L.; RIBEIRO-BARROS, A. I.; SILVA, M. J.; MAIA, R.; MÁGUAS, C.; REBOREDO, F. H.; PESSOA, M. F.; morais, l. e.; SANGLARD, L. M. V.; ARAÚJO, W. L.; GHINI, R.; DaMATTA, F. M.; RAMALHO, J. C. |
Afiliação: |
PAULA SCOTTI-CAMPOS, Instituto Nacional Investigação Agrária e Veterinária; ISABEL P PAIS, Instituto Nacional Investigação Agrária e Veterinária; FERNANDO C LIDON, Universidade Nova de Lisboa; LIMA D MARTINS, Instituto Investigação Científica Tropical; MARCELO ANTONIO TOMAZ, UFES; JOSE NOBRE SEMEDO, Instituto Nacional Investigação Agrária e Veterinária; ANA PAULA RODRIGUES, Universidade de Lisboa; ANA S FORTUNATO, Instituto Investigação Científica Tropical; I PALOS, Instituto Investigação Científica Tropical; PAULA BATISTA-SANTOS, Instituto Investigação Científica Tropical; ANTONIO E LEITAO, Instituto Investigação Científica Tropical; E LOPES, Instituto Investigação Científica Tropical; MARIA CRISTINA SIMÕES-COSTA, Instituto Investigação Científica Tropical; LUIS GOULAO, Instituto Investigação Científica Tropical; ANA I RIBEIRO-BARROS, Instituto Investigação Científica Tropical; M J SILVA, Instituto Investigação Científica Tropical; R MAIA, Universidade de Lisboa; CRISTINA MAGUAS, Universidade de Lisboa; FERNANDO H REBOREDO, Universidade Nova de Lisboa; M F PESSOA, Universidade Nova de Lisboa; LEANDRO ELIAS MORAIS, UFV; LILIAN MARIA VINCIS PEREIRA SANGLARD, UFV; WAGNER L ARAUJO, UFV; RAQUEL GHINI, CNPMA; FABIO MURILO DAMATTA, UFV; JOSE C RAMALHO, Instituto Investigação Científica Tropical. |
Título: |
Thylakoid lipids changes may account for photosynthetic acclimation ability of two coffea species subjected to heat. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 42-47. |
Idioma: |
Inglês |
Conteúdo: |
Summary: Coffee is one of the world?s most traded agricultural products, and its production could be threatened by global warming. The aim of this work was to evaluate the effects of heat on photosynthetic activity and thylakoid membrane lipid dynamics, on genotypes of the two major coffee producing species. Potted plants from C. arabica L. cv. IPR108 and C. canephora Pierre Ex A. Froehner cv. Conilon Clone 153 were grown for 1 year under controlled conditions of temperature (25/20ºC, day/night), irradiance (650-800 ?mol m-2 s-1), RH (75%), photoperiod (12 h), and 380 ?L CO2 L-1. Thereafter, temperature was gradually raised to 42/34ºC (0.5ºC/ day), with a 7 days stabilization step at 31, 37 and 42ºC. Studies focused modifications of thylakoid lipid composition and photosynthetic performance. In CL153 photosynthetic capacity (Amax) was not affected until 42ºC (40% reduction). In IPR108 it was reduced 35 and 57% at 37ºC at 42ºC, respectively. Thylakoid electron transport rate for photosystems (PS) I and II increased (ca. 10-25%) up to 37ºC in both genotypes. At 42ºC only IPR108 presented depressed activities on PSII (15%) and PSI (18%). Under 37 and 42ºC, CL153 plants presented digalactosyldiacylglycerol (DGDG) (ca. 42%) and monogalactosyldiacylglycerol (MGDG) (28-34%) increases, while IPR108 showed higher MGDG at all temperatures. In CL153 less unsaturated DGDG and phosphatidylglycerol (PG) along with stable or increased DGDG/MGDG ratio, may have contributed to sustain thylakoid electron flow at 37ºC and even 42ºC. IPR108 displayed a strong PG rise at all temperatures, in accordance with enhanced PSs activity. MenosSummary: Coffee is one of the world?s most traded agricultural products, and its production could be threatened by global warming. The aim of this work was to evaluate the effects of heat on photosynthetic activity and thylakoid membrane lipid dynamics, on genotypes of the two major coffee producing species. Potted plants from C. arabica L. cv. IPR108 and C. canephora Pierre Ex A. Froehner cv. Conilon Clone 153 were grown for 1 year under controlled conditions of temperature (25/20ºC, day/night), irradiance (650-800 ?mol m-2 s-1), RH (75%), photoperiod (12 h), and 380 ?L CO2 L-1. Thereafter, temperature was gradually raised to 42/34ºC (0.5ºC/ day), with a 7 days stabilization step at 31, 37 and 42ºC. Studies focused modifications of thylakoid lipid composition and photosynthetic performance. In CL153 photosynthetic capacity (Amax) was not affected until 42ºC (40% reduction). In IPR108 it was reduced 35 and 57% at 37ºC at 42ºC, respectively. Thylakoid electron transport rate for photosystems (PS) I and II increased (ca. 10-25%) up to 37ºC in both genotypes. At 42ºC only IPR108 presented depressed activities on PSII (15%) and PSI (18%). Under 37 and 42ºC, CL153 plants presented digalactosyldiacylglycerol (DGDG) (ca. 42%) and monogalactosyldiacylglycerol (MGDG) (28-34%) increases, while IPR108 showed higher MGDG at all temperatures. In CL153 less unsaturated DGDG and phosphatidylglycerol (PG) along with stable or increased DGDG/MGDG ratio, may have contributed to sustain thylak... Mostrar Tudo |
Thesagro: |
Aclimatação; Café; Clima; Coffea arabica; Dióxido de carbono; Fotossíntese; Temperatura. |
Thesaurus NAL: |
Carbon dioxide; Climate change; Photosynthesis; Temperature; Thylakoids. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137948/1/2015AA014.pdf
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Marc: |
LEADER 03313nam a2200553 a 4500 001 2035417 005 2016-01-28 008 2014 bl uuuu u00u1 u #d 100 1 $aSCOTTI-CAMPOS, P. 245 $aThylakoid lipids changes may account for photosynthetic acclimation ability of two coffea species subjected to heat.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 42-47.$c2014 520 $aSummary: Coffee is one of the world?s most traded agricultural products, and its production could be threatened by global warming. The aim of this work was to evaluate the effects of heat on photosynthetic activity and thylakoid membrane lipid dynamics, on genotypes of the two major coffee producing species. Potted plants from C. arabica L. cv. IPR108 and C. canephora Pierre Ex A. Froehner cv. Conilon Clone 153 were grown for 1 year under controlled conditions of temperature (25/20ºC, day/night), irradiance (650-800 ?mol m-2 s-1), RH (75%), photoperiod (12 h), and 380 ?L CO2 L-1. Thereafter, temperature was gradually raised to 42/34ºC (0.5ºC/ day), with a 7 days stabilization step at 31, 37 and 42ºC. Studies focused modifications of thylakoid lipid composition and photosynthetic performance. In CL153 photosynthetic capacity (Amax) was not affected until 42ºC (40% reduction). In IPR108 it was reduced 35 and 57% at 37ºC at 42ºC, respectively. Thylakoid electron transport rate for photosystems (PS) I and II increased (ca. 10-25%) up to 37ºC in both genotypes. At 42ºC only IPR108 presented depressed activities on PSII (15%) and PSI (18%). Under 37 and 42ºC, CL153 plants presented digalactosyldiacylglycerol (DGDG) (ca. 42%) and monogalactosyldiacylglycerol (MGDG) (28-34%) increases, while IPR108 showed higher MGDG at all temperatures. In CL153 less unsaturated DGDG and phosphatidylglycerol (PG) along with stable or increased DGDG/MGDG ratio, may have contributed to sustain thylakoid electron flow at 37ºC and even 42ºC. IPR108 displayed a strong PG rise at all temperatures, in accordance with enhanced PSs activity. 650 $aCarbon dioxide 650 $aClimate change 650 $aPhotosynthesis 650 $aTemperature 650 $aThylakoids 650 $aAclimatação 650 $aCafé 650 $aClima 650 $aCoffea arabica 650 $aDióxido de carbono 650 $aFotossíntese 650 $aTemperatura 700 1 $aPAIS, I. P. 700 1 $aLIDON, F. C. 700 1 $aMARTINS, L. A. 700 1 $aTOMAZ, M. A. 700 1 $aSEMEDO, J. N. 700 1 $aRODRIGUES, A. P. 700 1 $aFORTUNATO, A. S. 700 1 $aPALOS, I. 700 1 $aBATISTA-SANTOS, P. 700 1 $aLEITÃO, A. E. 700 1 $aLOPES, E. 700 1 $aSIMÕES-COSTA, M. C. 700 1 $aGOULÃO, L. 700 1 $aRIBEIRO-BARROS, A. I. 700 1 $aSILVA, M. J. 700 1 $aMAIA, R. 700 1 $aMÁGUAS, C. 700 1 $aREBOREDO, F. H. 700 1 $aPESSOA, M. F. 700 1 $amorais, l. e. 700 1 $aSANGLARD, L. M. V. 700 1 $aARAÚJO, W. L. 700 1 $aGHINI, R. 700 1 $aDaMATTA, F. M. 700 1 $aRAMALHO, J. C.
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