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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
24/09/2003 |
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Data da última atualização: |
30/03/2023 |
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Autoria: |
FREITAS-ASTUA, J.; ASTUA-MONGE, G.; POLSTON, J. E.; HIEBERT, E. |
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Afiliação: |
Juliana Freitas-Astua, CNPMS; Gustavo Astua-Monge, Instituto Agronômico/Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira; Jane Elisabeth Polston, Universidade Fluminense - UF/Gulf Coast Research & Education Center; Ernest Hiebert, Universidade Fluminense - UF/Plant Pathology. |
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Título: |
A simple and reliable method for the screening of transgenic tobacco plants. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 7, p. 893-896, jul. 2003 |
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Idioma: |
Inglês |
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Notas: |
Notas Científicas. Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas. |
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Conteúdo: |
Even though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still time-consuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the polymerase chain reaction (PCR) amplification of the transgene using specific primers. |
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Palavras-Chave: |
canamicina; organismo geneticamente modificado; teste. |
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Thesagro: |
Planta Transgênica. |
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Thesaurus Nal: |
genetically modified organisms; kanamycin; testing; transgenic plants. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/24881/1/v38n7a15.pdf
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Marc: |
LEADER 01761naa a2200265 a 4500
001 1109131
005 2023-03-30
008 2003 bl uuuu u00u1 u #d
100 1 $aFREITAS-ASTUA, J.
245 $aA simple and reliable method for the screening of transgenic tobacco plants.$h[electronic resource]
260 $c2003
500 $aNotas Científicas. Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas.
520 $aEven though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still time-consuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the polymerase chain reaction (PCR) amplification of the transgene using specific primers.
650 $agenetically modified organisms
650 $akanamycin
650 $atesting
650 $atransgenic plants
650 $aPlanta Transgênica
653 $acanamicina
653 $aorganismo geneticamente modificado
653 $ateste
700 1 $aASTUA-MONGE, G.
700 1 $aPOLSTON, J. E.
700 1 $aHIEBERT, E.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 38, n. 7, p. 893-896, jul. 2003
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
12/11/1998 |
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Data da última atualização: |
14/01/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SARGENT, S. A.; CORRÊA, T. S. B.; SOARES, A. G. |
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Afiliação: |
ANTONIO GOMES SOARES, CTAA. |
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Título: |
Application of postharvest coatings to fresh cassava roots (Manihot esculenta. Crantz) for reduction of vascular streaking. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF HARVEST AND POSTHARVEST TECHNOLOGIES FOR FRESH FRUITS AND VEGETABLES, 1995, Guanajuato. Proceedings... St. Joseph, MI: ASAE, 1995. p. 331-339. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Manihot esculenta Crantz. |
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Thesagro: |
Doença; Mandioca; Pós-Colheita; Tratamento. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/410426/1/1995016.pdf
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Marc: |
LEADER 00701nam a2200181 a 4500
001 1410426
005 2014-01-14
008 1995 bl uuuu u00u1 u #d
100 1 $aSARGENT, S. A.
245 $aApplication of postharvest coatings to fresh cassava roots (Manihot esculenta. Crantz) for reduction of vascular streaking.
260 $aIn: INTERNATIONAL CONFERENCE OF HARVEST AND POSTHARVEST TECHNOLOGIES FOR FRESH FRUITS AND VEGETABLES, 1995, Guanajuato. Proceedings... St. Joseph, MI: ASAE, 1995. p. 331-339.$c1995
650 $aDoença
650 $aMandioca
650 $aPós-Colheita
650 $aTratamento
653 $aManihot esculenta Crantz
700 1 $aCORRÊA, T. S. B.
700 1 $aSOARES, A. G.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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