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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Rondônia; Embrapa Semiárido. |
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Data corrente: |
26/02/2013 |
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Data da última atualização: |
27/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERRÃO, L. F. V.; CAIXETA, E. T.; SOUZA, F. de F.; ZAMBOLIM, E. M.; CRUZ, C. D.; ZABOLIM, L.; SAKIYAMA, N. S. |
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Afiliação: |
LUÍS FELIPE V. FERRÃO, Instituto de Biotecnologia Aplicada á Agropecuária(BIOAGRO) Laboratório BIOCAFÉ, Universidade Federal de Viçosa,MG; EVELINE TEIXEIRA CAIXETA, SAPC; FLAVIO DE FRANCA SOUZA, CPATSA; EUNIZE M.ZAMBOLIM, Instituto de Biotecnologia Aplicada áAgropecuária(BIOAGRO), Laboratório BIOCAFÉ, Universidade Federal de Viçosa, MG; COSME D.CRUZ, Departamento de Biologia Geral, Universidade Federal de Viçosa, MG; LAÉRCIO ZAMBOLIM, Departamento de Fitopatologia, Univerdade Federal de Viçosa, MG; NEY S. SAKIYAMA, Departamento de Fitotecnia, Universidade Federal de Viçosa, MG. |
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Título: |
Comparative study of different molecular markers for classifying and establishing genetic relationshps in Coffea Canephora. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Plant Systematics and Evolution, Springer Verlag, New York, v. 299, p. 225-238, 2013. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
The genetic variability characterization of the accessions of the germplasm collection, using molecular markers, is being applied as a complementary strategy to the traditional approaches to redefine the plant genetic resources. In this study, we compared the informativeness and efficiency of the molecular markers RAPD, AFLP and SSR in the analysis of 94 accessions of Coffea canephora germplasm held by the breeding program of the Brazilian Agricultural Research Corporation (Embrapa), Rondonia State, Brazil. For this, we considered the marker?s discriminatory power and level of polymorphism detected and also the genetic relationships and clustering (dendrogram) analysis. The RAPD marker yielded low-quality data and problems in the discrimination of some accessions, being less recommended for genetic studies of C. canephora . The SSRs had a higher level of information content and yielded high-quality data, while AFLP was the most efficient marker system because of the simultaneous detection of abundant polymorphism markers per few reactions. Our results indicate that AFLP and SSR, allies to the intrinsic characteristics of each technique, are the most suitable molecular markers for genetic studies of C. canephora .However, the choice of AFLP or SSR in the species characterization should be made in agreement with some characteristics that are discussed in this work. |
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Palavras-Chave: |
AFLP; Coffee; Melhoramento genético; RAPD; Robusta coffee; SSR. |
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Thesagro: |
Café. |
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Thesaurus Nal: |
genetic relationships. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173275/1/Flvio-2013.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216288/1/Ferrao-et-al-2013-1.pdf
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Marc: |
LEADER 02242naa a2200289 a 4500
001 2088232
005 2018-02-27
008 2013 bl uuuu u00u1 u #d
100 1 $aFERRÃO, L. F. V.
245 $aComparative study of different molecular markers for classifying and establishing genetic relationshps in Coffea Canephora.$h[electronic resource]
260 $c2013
520 $aThe genetic variability characterization of the accessions of the germplasm collection, using molecular markers, is being applied as a complementary strategy to the traditional approaches to redefine the plant genetic resources. In this study, we compared the informativeness and efficiency of the molecular markers RAPD, AFLP and SSR in the analysis of 94 accessions of Coffea canephora germplasm held by the breeding program of the Brazilian Agricultural Research Corporation (Embrapa), Rondonia State, Brazil. For this, we considered the marker?s discriminatory power and level of polymorphism detected and also the genetic relationships and clustering (dendrogram) analysis. The RAPD marker yielded low-quality data and problems in the discrimination of some accessions, being less recommended for genetic studies of C. canephora . The SSRs had a higher level of information content and yielded high-quality data, while AFLP was the most efficient marker system because of the simultaneous detection of abundant polymorphism markers per few reactions. Our results indicate that AFLP and SSR, allies to the intrinsic characteristics of each technique, are the most suitable molecular markers for genetic studies of C. canephora .However, the choice of AFLP or SSR in the species characterization should be made in agreement with some characteristics that are discussed in this work.
650 $agenetic relationships
650 $aCafé
653 $aAFLP
653 $aCoffee
653 $aMelhoramento genético
653 $aRAPD
653 $aRobusta coffee
653 $aSSR
700 1 $aCAIXETA, E. T.
700 1 $aSOUZA, F. de F.
700 1 $aZAMBOLIM, E. M.
700 1 $aCRUZ, C. D.
700 1 $aZABOLIM, L.
700 1 $aSAKIYAMA, N. S.
773 $tPlant Systematics and Evolution, Springer Verlag, New York$gv. 299, p. 225-238, 2013.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 1 | |
| 1. |  | FERRÃO, L. F. V.; CAIXETA, E. T.; SOUZA, F. de F.; ZAMBOLIM, E. M.; CRUZ, C. D.; ZABOLIM, L.; SAKIYAMA, N. S. Comparative study of different molecular markers for classifying and establishing genetic relationshps in Coffea Canephora. Plant Systematics and Evolution, Springer Verlag, New York, v. 299, p. 225-238, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café; Embrapa Rondônia; Embrapa Semiárido. |
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