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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
04/01/2018 |
Data da última atualização: |
04/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, M. F. C.; SILVA, C. R. C.; LIMA, L. M. de; SANTOS, R. C. dos; RAMOS, J. P. C. |
Afiliação: |
M. F. C. SILVA, UEPB; C. R. C. SILVA, UFRPE - RENORBIO; LIZIANE MARIA DE LIMA, CNPA; ROSEANE CAVALCANTI DOS SANTOS, CNPA; JEAN PIERRE CORDEIRO RAMOS, UFPB. |
Título: |
Differential expression of dormancy-associated genes in fastigiata and hypogaea peanut. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n. 4, gmr 16039820, 2017. |
Idioma: |
Inglês |
Conteúdo: |
Seed dormancy is a temporary replacement state observed in some higher plants, which prevents germination under unfavorable conditions. In several species, dormancy process is triggered by hormonal factors, initiated by ABA and precursor enzymes. In this work, we investigated the relationship between dormancy and the expression of ARP, DMR1, and NCED genes in upright and runner peanut seeds. Eight dormancy-contrasting genotypes were previously phenotyped based on germination traits, during three storage periods. Expression of ARP, DMR1 and NCED transcripts was analyzed by qRT-PCR, from endosperm and embryo tissues. Higher expression of NCED and ARP were found in embryo tissues from runner genotypes, IAC Caiapo and LGoPE-06, confirming findings in literature as to association of these genes with seed dormancy. Higher expression of DMR1 was found only in endosperm of upright materials (L7 Bege and BR1). We suggest the NCED is a functional molecular marker to identify dormancy in seeds, contributing to selection procedures of runner genotypes in peanut breeding. |
Palavras-Chave: |
Embryo; Molecular markers. |
Thesagro: |
Amendoim; Arachis Hypogaea; Dormência da semente; Embrião; Endosperma; Marcador molecular. |
Thesaurus Nal: |
Endosperm; Genes; Peanuts; Seed dormancy. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170349/1/Differential-expression-....pdf
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Marc: |
LEADER 01947naa a2200313 a 4500 001 2084266 005 2018-01-04 008 2017 bl uuuu u00u1 u #d 100 1 $aSILVA, M. F. C. 245 $aDifferential expression of dormancy-associated genes in fastigiata and hypogaea peanut.$h[electronic resource] 260 $c2017 520 $aSeed dormancy is a temporary replacement state observed in some higher plants, which prevents germination under unfavorable conditions. In several species, dormancy process is triggered by hormonal factors, initiated by ABA and precursor enzymes. In this work, we investigated the relationship between dormancy and the expression of ARP, DMR1, and NCED genes in upright and runner peanut seeds. Eight dormancy-contrasting genotypes were previously phenotyped based on germination traits, during three storage periods. Expression of ARP, DMR1 and NCED transcripts was analyzed by qRT-PCR, from endosperm and embryo tissues. Higher expression of NCED and ARP were found in embryo tissues from runner genotypes, IAC Caiapo and LGoPE-06, confirming findings in literature as to association of these genes with seed dormancy. Higher expression of DMR1 was found only in endosperm of upright materials (L7 Bege and BR1). We suggest the NCED is a functional molecular marker to identify dormancy in seeds, contributing to selection procedures of runner genotypes in peanut breeding. 650 $aEndosperm 650 $aGenes 650 $aPeanuts 650 $aSeed dormancy 650 $aAmendoim 650 $aArachis Hypogaea 650 $aDormência da semente 650 $aEmbrião 650 $aEndosperma 650 $aMarcador molecular 653 $aEmbryo 653 $aMolecular markers 700 1 $aSILVA, C. R. C. 700 1 $aLIMA, L. M. de 700 1 $aSANTOS, R. C. dos 700 1 $aRAMOS, J. P. C. 773 $tGenetics and Molecular Research$gv. 16, n. 4, gmr 16039820, 2017.
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Embrapa Algodão (CNPA) |
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