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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/11/2017 |
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Data da última atualização: |
23/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, S. V. F.; PORTUGAL, L. A.; ANJOS, J. P. dos; JESUS, O. N. de; OLIVEIRA, E. J. de; DAVID, J. P.; DAVID, J. M. |
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Afiliação: |
SILVANA V. F. GOMES, UFBA; LINDOMAR A. PORTUGAL, UFBA; JEANCARLO P. DOS ANJOS, UFBA; ONILDO NUNES DE JESUS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF; JUCENI P. DAVID, UFBA; JORGE M. DAVID, UFBA. |
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Título: |
Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Microchemical Journal, V.132, P.28-35, 2017. |
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ISSN: |
0026-265X |
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Idioma: |
Inglês |
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Conteúdo: |
This work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively. |
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Thesagro: |
Maracujá. |
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Thesaurus Nal: |
Passiflora. |
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Categoria do assunto: |
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Marc: |
LEADER 02017naa a2200229 a 4500
001 2079765
005 2017-11-23
008 2017 bl uuuu u00u1 u #d
022 $a0026-265X
100 1 $aGOMES, S. V. F.
245 $aAccelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species.$h[electronic resource]
260 $c2017
520 $aThis work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively.
650 $aPassiflora
650 $aMaracujá
700 1 $aPORTUGAL, L. A.
700 1 $aANJOS, J. P. dos
700 1 $aJESUS, O. N. de
700 1 $aOLIVEIRA, E. J. de
700 1 $aDAVID, J. P.
700 1 $aDAVID, J. M.
773 $tMicrochemical Journal, V.132, P.28-35, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/01/2016 |
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Data da última atualização: |
20/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LEAL-BERTIOLI, S. C. M.; MORETZSOHN, M. C.; ROBERTS, P. A.; BALLEN-TABORDA, C.; BORBA, T. C. O.; VALDISSER, P. A.; VIANELLO, R. P.; ARAUJO, A. C. G.; GUIMARAES, P. M.; BERTIOLI, D. J. |
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Afiliação: |
SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; MARCIO DE CARVALHO MORETZSOHN, CENARGEN; PHILIP A. ROBERTS, University of California; CAROLINA BALLEN-TABORDA, University of Georgia; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; DAVID J. BERTIOLI, University of Georgia. |
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Título: |
Genetic mapping of resistance to Meloidogyne arenaria in arachis stenosperma: a new source of nematode resistance for peanut. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
G3: Genes, Genomes, Genetics, v. 12, 2015. |
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DOI: |
10.1534/g3.115.023044 |
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Idioma: |
Inglês |
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Conteúdo: |
Root-knot nematodes (RKN; Meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. The use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. Peanut (Arachis hypogaea) is infected by four species of RKN, the most damaging being M. arenaria, and commercial cultivars rely on a single source of resistance. In this study, we genetically characterize RKN resistance of the wild Arachis species A. stenosperma using a population of 93 recombinant inbred lines developed from a cross between A. duranensis and A. stenosperma. Four quantitative trait loci (QTL) located on linkage groups 02, 04, and 09 strongly influenced nematode root galling and egg production. Drought-related, domestication and agronomically relevant traits were also evaluated, revealing several QTL. Using the newly available Arachis genome sequence, easy-to-use KASP (kompetitive allele specific PCR) markers linked to the newly identified RKN resistance loci were developed and validated in a tetraploid context. Therefore, we consider that A. stenosperma has high potential as a new source of RKN resistance in peanut breeding programs. |
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Palavras-Chave: |
Marker-assisted; Nematode; Peanut; QTL; Root-knot. |
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Thesagro: |
Amendoim; Meloidogyne arenaria; Nematoide. |
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Thesaurus NAL: |
Arachis; Chromosome mapping; drought; Marker-assisted selection; Nematoda; Peanuts; Quantitative trait loci; Root-knot nematodes. |
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Categoria do assunto: |
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S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158635/1/Genetic-mapping-of-resistance-to-Meloidogyne-arenaria-in-Arachis-stenosperma....pdf
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Marc: |
LEADER 02460naa a2200433 a 4500
001 2033044
005 2023-03-20
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1534/g3.115.023044$2DOI
100 1 $aLEAL-BERTIOLI, S. C. M.
245 $aGenetic mapping of resistance to Meloidogyne arenaria in arachis stenosperma$ba new source of nematode resistance for peanut.$h[electronic resource]
260 $c2015
520 $aRoot-knot nematodes (RKN; Meloidogyne sp.) are a major threat to crops in tropical and subtropical regions worldwide. The use of resistant crop varieties is the preferred method of control because nematicides are expensive, and hazardous to humans and the environment. Peanut (Arachis hypogaea) is infected by four species of RKN, the most damaging being M. arenaria, and commercial cultivars rely on a single source of resistance. In this study, we genetically characterize RKN resistance of the wild Arachis species A. stenosperma using a population of 93 recombinant inbred lines developed from a cross between A. duranensis and A. stenosperma. Four quantitative trait loci (QTL) located on linkage groups 02, 04, and 09 strongly influenced nematode root galling and egg production. Drought-related, domestication and agronomically relevant traits were also evaluated, revealing several QTL. Using the newly available Arachis genome sequence, easy-to-use KASP (kompetitive allele specific PCR) markers linked to the newly identified RKN resistance loci were developed and validated in a tetraploid context. Therefore, we consider that A. stenosperma has high potential as a new source of RKN resistance in peanut breeding programs.
650 $aArachis
650 $aChromosome mapping
650 $adrought
650 $aMarker-assisted selection
650 $aNematoda
650 $aPeanuts
650 $aQuantitative trait loci
650 $aRoot-knot nematodes
650 $aAmendoim
650 $aMeloidogyne arenaria
650 $aNematoide
653 $aMarker-assisted
653 $aNematode
653 $aPeanut
653 $aQTL
653 $aRoot-knot
700 1 $aMORETZSOHN, M. C.
700 1 $aROBERTS, P. A.
700 1 $aBALLEN-TABORDA, C.
700 1 $aBORBA, T. C. O.
700 1 $aVALDISSER, P. A.
700 1 $aVIANELLO, R. P.
700 1 $aARAUJO, A. C. G.
700 1 $aGUIMARAES, P. M.
700 1 $aBERTIOLI, D. J.
773 $tG3: Genes, Genomes, Genetics$gv. 12, 2015.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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