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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
25/10/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, C. J. M. de; FAJARDO, T. V. M.; EIRAS, M.; SILVA, F. N. da; NICKEL, O. |
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Afiliação: |
Cátia Jacira Martins de Moura, 1Biological Institute ? Dept. of Plant Pathology ? Lab. of Plant Virology, Av. Conselheiro Rodrigues Alves, 1252 ? 04014-900 ? São Paulo, SP ? Brazil.; THOR VINICIUS MARTINS FAJARDO, CNPUV; Marcelo Eiras, 1Biological Institute ? Dept. of Plant Pathology ? Lab. of Plant Virology, Av. Conselheiro Rodrigues Alves, 1252 ? 04014-900 ? São Paulo, SP ? Brazil.; Fábio Nascimento da Silva, 3Santa Catarina State University/Centre of Agroveterinary Sciences, Av. Luiz de Camões, 2090 ? 88520-000 ? Lages, SC ? Brazil.; OSMAR NICKEL, CNPUV. |
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Título: |
Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Scientia Agricola, Brasília,DF, v. 75, n. 1, p.43-51, jan./feb. 2018. |
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DOI: |
10.1590/1678-992X-2016-0328 |
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Idioma: |
Inglês |
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Conteúdo: |
The aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease. Keywords: Vitis, diagnosis, variability, incidence, leafroll MenosThe aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pat... Mostrar Tudo |
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Palavras-Chave: |
Caracterização molecular; Cultivo de uva; Diagnosis; Leafroll; Variabilitty; Videira; Vírus em videira. |
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Thesagro: |
DOença de planta; Uva. |
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Thesaurus Nal: |
incidence; Vitis. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165554/1/SA-Molecular-characterization-GSyV1-and-GLRaV3-2018.pdf
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Marc: |
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024 7 $a10.1590/1678-992X-2016-0328$2DOI
100 1 $aMOURA, C. J. M. de
245 $aMolecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area.$h[electronic resource]
260 $c2018
520 $aThe aims of this study were to determine the prevalence of viruses in 119 samples from 32 grapevine cultivars, collected from nine vineyards in a specific grape-growing area in southeastern Brazil, perform a partial molecular characterization of 14 isolates of Grapevine Syrah virus 1 (GSyV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) and assess the coat protein genetic variability of these viruses. The detection of viruses was implemented by realtime RT-PCR (reverse transcription polymerase chain reaction) aiming to detect seven viruses and one viroid. With the exception of the Grapevine Cabernet Sauvignon reovirus (GCSV), the viruses and viroid that were evaluated were widespread in the sampled areas, often in high prevalence and multiple infections, ranging from 15 % up to 76 %. Eight isolates of GSyV-1 and six of GLRaV-3, partially characterized by complete coat protein gene nucleotide sequencing and a variability study showed nucleotide identities ranging from 91 % to 99 % (GSyV-1) and from 98 % to 100 % (GLRaV-3) among themselves, respectively. Comparisons between conventional and real-time RT-PCR detections were implemented for GSyV-1 and GLRaV-3 infections. Analysis of genetic variability indicated molecular differences between GSyV-1 and GLRaV-3 isolates and negative selection acting on the coat protein gene of both viruses. This is the first report of GSyV-1 in commercial vineyards in Brazil. The survey revealed widespread infections of seven important pathogens in one prominent Brazilian grape-producing region implying contaminated grapevine cuttings in the spread of disease. Keywords: Vitis, diagnosis, variability, incidence, leafroll
650 $aincidence
650 $aVitis
650 $aDOença de planta
650 $aUva
653 $aCaracterização molecular
653 $aCultivo de uva
653 $aDiagnosis
653 $aLeafroll
653 $aVariabilitty
653 $aVideira
653 $aVírus em videira
700 1 $aFAJARDO, T. V. M.
700 1 $aEIRAS, M.
700 1 $aSILVA, F. N. da
700 1 $aNICKEL, O.
773 $tScientia Agricola, Brasília,DF$gv. 75, n. 1, p.43-51, jan./feb. 2018.
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Embrapa Uva e Vinho (CNPUV) |
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| 1. |  | MOURA, C. J. M. de; FAJARDO, T. V. M.; EIRAS, M.; SILVA, F. N. da; NICKEL, O. Molecular characterization of GSyV-1 and GLRaV-3 and prevalence of grapevine viruses in a grape-growing area. Scientia Agricola, Brasília,DF, v. 75, n. 1, p.43-51, jan./feb. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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