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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
27/09/2017 |
Data da última atualização: |
15/04/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CAVALCANTE, R. V.; NOGUEIRA, M. F.; ARAÚJO Jr. J. P. |
Afiliação: |
MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP. |
Título: |
Evaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Virus Reviews & Research, v. 18, p. 219, 2013. Supplement 1. |
Idioma: |
Inglês |
Notas: |
Trabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA. |
Conteúdo: |
Equine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques. |
Palavras-Chave: |
Anemia Infecciosa Equina; EIAV (Equine infectious anemia virus). |
Thesagro: |
Eqüino; Virus. |
Thesaurus Nal: |
Epidemiology; Equine infectious anemia; Horse diseases. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164439/1/VV218.pdf
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Marc: |
LEADER 01615nam a2200229 a 4500 001 2076399 005 2019-04-15 008 2013 bl uuuu u00u1 u #d 100 1 $aCAVALCANTE, R. V. 245 $aEvaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil.$h[electronic resource] 260 $aVirus Reviews & Research, v. 18, p. 219, 2013. Supplement 1.$c2013 500 $aTrabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA. 520 $aEquine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques. 650 $aEpidemiology 650 $aEquine infectious anemia 650 $aHorse diseases 650 $aEqüino 650 $aVirus 653 $aAnemia Infecciosa Equina 653 $aEIAV (Equine infectious anemia virus) 700 1 $aNOGUEIRA, M. F. 700 1 $aARAÚJO Jr. J. P.
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Embrapa Pantanal (CPAP) |
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