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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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Data corrente: |
06/07/2017 |
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Data da última atualização: |
21/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, K. B.; FACCIOLI, P. Y.; BONESSO, M. F.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. |
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Afiliação: |
Katheryne Benini Martins, UNESP; Patricia Yoshida Faccioli, UNESP; Mariana Fávero Bonesso, UNESP; Simone Fernandes, UNESP; Aline A. Oliveira, UNESP; Ariane Dantas, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Maria de Lourdes Ribeiro de Souza da Cunha, UNESP. |
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Título: |
Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 100, n. 3, p. 2184-2195, 2017. |
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ISSN: |
0022-0302 |
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DOI: |
https://doi.org/10.3168/jds.2016-11583 |
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Idioma: |
Inglês |
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Conteúdo: |
Coagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey of toxin genes has shown that 70 (62.5%) samples showed some toxin-encoding gene. However, none of the samples has expressed any of the genes from those toxins studied. MenosCoagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey... Mostrar Tudo |
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Palavras-Chave: |
Coagulase-negative staphylococci; Resistance; Sheep mastitis. |
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Thesaurus Nal: |
Biofilm; Disease resistance; Mastitis; Sheep diseases; Toxins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02716naa a2200325 a 4500
001 2072041
005 2017-11-21
008 2017 bl uuuu u00u1 u #d
022 $a0022-0302
024 7 $ahttps://doi.org/10.3168/jds.2016-11583$2DOI
100 1 $aMARTINS, K. B.
245 $aCharacteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis.$h[electronic resource]
260 $c2017
520 $aCoagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey of toxin genes has shown that 70 (62.5%) samples showed some toxin-encoding gene. However, none of the samples has expressed any of the genes from those toxins studied.
650 $aBiofilm
650 $aDisease resistance
650 $aMastitis
650 $aSheep diseases
650 $aToxins
653 $aCoagulase-negative staphylococci
653 $aResistance
653 $aSheep mastitis
700 1 $aFACCIOLI, P. Y.
700 1 $aBONESSO, M. F.
700 1 $aFERNANDES, S.
700 1 $aOLIVEIRA, A. A.
700 1 $aDANTAS, A.
700 1 $aZAFALON, L. F.
700 1 $aCUNHA, M. de L. R. de S. da
773 $tJournal of Dairy Science$gv. 100, n. 3, p. 2184-2195, 2017.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/09/1995 |
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Data da última atualização: |
20/06/1996 |
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Autoria: |
CORREA-FERREIRA, B.S. |
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Afiliação: |
EMBRAPA-CNPSo. Londrina, PR. |
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Título: |
Controle biologico de percevejos da soja. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
In: CICLO DE PALESTRAS SOBRE CONTROLE BIOLOGICO DE PRAGAS, 3., 1993, Campinas. Anais... Campinas: Instituto Biologico, 1993. |
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Páginas: |
p.41-49. |
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Idioma: |
Português |
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Conteúdo: |
Incidencia natural dos parasitoides de ovos de percevejos; Biologia de Trissolcus basalis; Producao massal do parasitoide Trissolcus basalis; Criacao do hospedeiro; Multiplicacao do parasitoide; Uso e eficiencia de controle; Programa ao nivel de agricultor. |
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Palavras-Chave: |
Egg parasite; Insect pest; Inseto praga; Parasita de ovos; Producao massal; Soybean. |
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Thesagro: |
Biologia; Controle Biológico; Euschistus Heros; Inimigo Natural; Inseto; Nezara Viridula; Percevejo; Piezodorus Guildinii; Praga; Soja. |
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Thesaurus NAL: |
biological control; natural enemies; Trissolcus basalis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01243naa a2200361 a 4500
001 1456084
005 1996-06-20
008 1993 bl uuuu u00u1 u #d
100 1 $aCORREA-FERREIRA, B.S.
245 $aControle biologico de percevejos da soja.
260 $c1993
300 $ap.41-49.
520 $aIncidencia natural dos parasitoides de ovos de percevejos; Biologia de Trissolcus basalis; Producao massal do parasitoide Trissolcus basalis; Criacao do hospedeiro; Multiplicacao do parasitoide; Uso e eficiencia de controle; Programa ao nivel de agricultor.
650 $abiological control
650 $anatural enemies
650 $aTrissolcus basalis
650 $aBiologia
650 $aControle Biológico
650 $aEuschistus Heros
650 $aInimigo Natural
650 $aInseto
650 $aNezara Viridula
650 $aPercevejo
650 $aPiezodorus Guildinii
650 $aPraga
650 $aSoja
653 $aEgg parasite
653 $aInsect pest
653 $aInseto praga
653 $aParasita de ovos
653 $aProducao massal
653 $aSoybean
773 $tIn: CICLO DE PALESTRAS SOBRE CONTROLE BIOLOGICO DE PRAGAS, 3., 1993, Campinas. Anais... Campinas: Instituto Biologico, 1993.
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Embrapa Soja (CNPSO) |
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