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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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Data corrente: |
06/07/2017 |
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Data da última atualização: |
21/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, K. B.; FACCIOLI, P. Y.; BONESSO, M. F.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. |
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Afiliação: |
Katheryne Benini Martins, UNESP; Patricia Yoshida Faccioli, UNESP; Mariana Fávero Bonesso, UNESP; Simone Fernandes, UNESP; Aline A. Oliveira, UNESP; Ariane Dantas, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Maria de Lourdes Ribeiro de Souza da Cunha, UNESP. |
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Título: |
Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 100, n. 3, p. 2184-2195, 2017. |
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ISSN: |
0022-0302 |
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DOI: |
https://doi.org/10.3168/jds.2016-11583 |
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Idioma: |
Inglês |
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Conteúdo: |
Coagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey of toxin genes has shown that 70 (62.5%) samples showed some toxin-encoding gene. However, none of the samples has expressed any of the genes from those toxins studied. MenosCoagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey... Mostrar Tudo |
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Palavras-Chave: |
Coagulase-negative staphylococci; Resistance; Sheep mastitis. |
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Thesaurus Nal: |
Biofilm; Disease resistance; Mastitis; Sheep diseases; Toxins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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001 2072041
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022 $a0022-0302
024 7 $ahttps://doi.org/10.3168/jds.2016-11583$2DOI
100 1 $aMARTINS, K. B.
245 $aCharacteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis.$h[electronic resource]
260 $c2017
520 $aCoagulase-negative staphylococci (CNS) are among the main responsible agents for mastitis in sheep. Cure rates can be reduced due to several causes, such as those related to virulence factors presented by microorganisms. This study aims at characterizing the virulence and resistance factors to antimicrobial agents in different CNS species isolated from sheep milk. After collecting milk samples, the samples were analyzed and the CNS species were identified. After identification, the susceptibility-sensitivity profile was examined using the disk diffusion technique for 10 antimicrobial agents. The DNA was extracted to detect the presence of the mecA gene, biofilm (icaADBC, bap, and bhp) and toxin genes (sea, seb, sec, sed, tst, and luk-PV) by PCR. Samples carrying toxin genes had their expression assessed using the reverse-transcription PCR technique. The biofilm production was assessed using the adherence method on a polystyrene plate. One hundred twelve CNS samples were isolated, 53 (47.3%) from animals with subclinical mastitis and 59 (52.7%) from healthy animals. Drugs tested have shown to be efficient for most CNS samples. The largest resistance percentage of CNS was found for the penicillin (17.0%) and tetracycline (10.7%) and 4 samples carried the mecA gene. As for the biofilm genes, the icaADBC operon was found in 10 (8.9%) samples, the bap gene was found in 16 (14.3%), and the bhp gene was found in 3 (2.7%). In addition, 69 (61.6%) samples produced biofilm. The survey of toxin genes has shown that 70 (62.5%) samples showed some toxin-encoding gene. However, none of the samples has expressed any of the genes from those toxins studied.
650 $aBiofilm
650 $aDisease resistance
650 $aMastitis
650 $aSheep diseases
650 $aToxins
653 $aCoagulase-negative staphylococci
653 $aResistance
653 $aSheep mastitis
700 1 $aFACCIOLI, P. Y.
700 1 $aBONESSO, M. F.
700 1 $aFERNANDES, S.
700 1 $aOLIVEIRA, A. A.
700 1 $aDANTAS, A.
700 1 $aZAFALON, L. F.
700 1 $aCUNHA, M. de L. R. de S. da
773 $tJournal of Dairy Science$gv. 100, n. 3, p. 2184-2195, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 1. |  | MARTINS, K. B.; FACCIOLI, P. Y.; BONESSO, M. F.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. Journal of Dairy Science, v. 100, n. 3, p. 2184-2195, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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