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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
16/03/2017 |
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Data da última atualização: |
15/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, T. V.; CAIXETA, E. T.; ALKIMIM, E. R.; OLIVEIRA, A. C. B. de; PEREIRA, A. A.; ZAMBOLIM, L.; SAKIYAMA, N. S. |
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Afiliação: |
TIAGO VIEIRA SOUSA, BIOAGRO, Biocafé/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; EMILLY RUAS ALKIMIM, BIOAGRO, Biocafé/UFV; ANTONIO CARLOS BAIAO DE OLIVEIRA, SAPC; ANTONIO ALVES PEREIRA, EPAMIG; LAÉRCIO ZAMBOLIM, UFV; NEY SUSSUMU SAKIYAMA, UFV. |
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Título: |
Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Euphytica, v. 213, n. 3, March, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars. MenosNew cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness ... Mostrar Tudo |
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Palavras-Chave: |
Cultivars register; Molecular profiling; Protection of plant cultivars; SSR marker. |
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Thesaurus Nal: |
Coffea; Cultivar identification; DNA fingerprinting; Genetic variation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157715/1/Molecular-markers-useful-to-discriminate-Coffea.pdf
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Marc: |
LEADER 02511naa a2200289 a 4500
001 2067171
005 2017-12-15
008 2017 bl uuuu u00u1 u #d
100 1 $aSOUSA, T. V.
245 $aMolecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity.$h[electronic resource]
260 $c2017
520 $aNew cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.
650 $aCoffea
650 $aCultivar identification
650 $aDNA fingerprinting
650 $aGenetic variation
653 $aCultivars register
653 $aMolecular profiling
653 $aProtection of plant cultivars
653 $aSSR marker
700 1 $aCAIXETA, E. T.
700 1 $aALKIMIM, E. R.
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aPEREIRA, A. A.
700 1 $aZAMBOLIM, L.
700 1 $aSAKIYAMA, N. S.
773 $tEuphytica$gv. 213, n. 3, March, 2017.
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Embrapa Café (CNPCa) |
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| 1. |  | SOUSA, T. V.; CAIXETA, E. T.; ALKIMIM, E. R.; OLIVEIRA, A. C. B. de; PEREIRA, A. A.; ZAMBOLIM, L.; SAKIYAMA, N. S. Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity. Euphytica, v. 213, n. 3, March, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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