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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
06/03/2017 |
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Data da última atualização: |
06/03/2017 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
MORGANTE, C. V.; BLAWID, R. |
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Afiliação: |
CAROLINA VIANNA MORGANTE, CPATSA; ROSANA BLAWID, Universidade de Brasília, Brasília, DF. |
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Título: |
Análise da expressão gênica pela técnica de PCR quantitativa em tempo real: princípios e fundamentos. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Petrolina: Embrapa Semiárido, 2016. |
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Páginas: |
63 p. |
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Descrição Física: |
il. |
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Série: |
(Embrapa Semiárido. Documentos, 278). |
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ISSN: |
1808-9992 |
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Idioma: |
Português |
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Conteúdo: |
Neste trabalho são apresentados os conceitos fundamentos da análise da expressão gênica pela técnica de PCR e reúne informações importantes para aqueles que se dedicam ao estudo da genética vegetal. Sumário: Introdução; Rermos básicos utilizados na qPCR; Sistemas para a detecção da fluorescência; Etapas anteriores à reação de qPCR; Parâmetros para a padronização da qPCR; Métodos de quantificação gênica; Referências; Glossário. |
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Palavras-Chave: |
Metodologia de análise; PCR. |
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Thesagro: |
Biologia molecular; Biotecnologia; Genética vegetal. |
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Thesaurus Nal: |
Biotechnology. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157145/1/SDC278.pdf
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Marc: |
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001 2066218
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008 2016 bl uuuu 00u1 u #d
022 $a1808-9992
100 1 $aMORGANTE, C. V.
245 $aAnálise da expressão gênica pela técnica de PCR quantitativa em tempo real$bprincípios e fundamentos.$h[electronic resource]
260 $aPetrolina: Embrapa Semiárido$c2016
300 $a63 p.$cil.
490 $a(Embrapa Semiárido. Documentos, 278).
520 $aNeste trabalho são apresentados os conceitos fundamentos da análise da expressão gênica pela técnica de PCR e reúne informações importantes para aqueles que se dedicam ao estudo da genética vegetal. Sumário: Introdução; Rermos básicos utilizados na qPCR; Sistemas para a detecção da fluorescência; Etapas anteriores à reação de qPCR; Parâmetros para a padronização da qPCR; Métodos de quantificação gênica; Referências; Glossário.
650 $aBiotechnology
650 $aBiologia molecular
650 $aBiotecnologia
650 $aGenética vegetal
653 $aMetodologia de análise
653 $aPCR
700 1 $aBLAWID, R.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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Tipo/Formato |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/09/2015 |
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Data da última atualização: |
02/01/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TONUCCI, R. G.; GARCIA, R.; NAIR, V. |
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Afiliação: |
RAFAEL GONCALVES TONUCCI, CNPC; Rasmo Garcia, Federal Univ. of Viçosa (UFV) - Viçosa, MG, Brazil; Vimala Nair, Soil and Water Science Dep. Univ. of Florida, Gainesville. |
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Título: |
Trees and grass contribution to soil organic carbon in agroforestry systems. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Agroforestry systems have the potential to enhance carbon (C) sequestration in soil compared with treeless (agricultural) systems (Montagnini & Nair, 2004). When one type of vegetation is replaced with another, stable isotope contents (?13 C) values can be used to identify soil organic carbon (SOC) derived from residues in the native vegetation and the new vegetation based on discrimination between C3 and C4 plants. The present study aimed to assessing the impact of difference land-use systems on C3 and C4 contribution to SOC. The experimental area is located inside the Cerrado biome. Soil samples were taken from six different land-use sites: (i) native local forest; (ii) Eucalyptus forest (EF) established in 1985 (OEC); (iii) EF established in 2004 (NEC); (iv) pasture of B. decumbens; (v) Agroforestry System (AF) established on 1994 (OAF); and (vi) AF established on 2004 (NAF). The establishment on AF was placed first with the eucalyptus planted and rice (Oryza sativa), soybean (Glycine max) and braquiaria grass (B. Brizantha cv. Marandu) in between trees rows. Soil was collected from four depths (0-10; 10-20; 20-50 and 50-100 cm). For stable C isotope analysis, whole soil was analyzed mass spectrometer. The percentage of SOC derived from the Brachiaria ssp., a C4 plant, or from the eucalyptus or native forest, a C3 plant, was estimated based on the equations: % C4-derived SOC = (?- ?T)/(?G- ?T) x 100 (1); % C3-derived SOC = 100 - % C4-derived SOC (2) Based on the equations 1 and 2 were calculated the contributions of each C3 and C4 species in SOC C-derived, as follows: C3-devived SOC (Mg ha-1) = (% C3-derived SOC) x (SOC content, Mg ha-1) (3); C4-derived SOC (Mg ha-1) = (% C4-derived SOC) x (SOC content, Mg ha-1) (4). A complete randomized design was used with Tukey?s studentized. Statistical differences were considered significant at p <0.05. MenosAgroforestry systems have the potential to enhance carbon (C) sequestration in soil compared with treeless (agricultural) systems (Montagnini & Nair, 2004). When one type of vegetation is replaced with another, stable isotope contents (?13 C) values can be used to identify soil organic carbon (SOC) derived from residues in the native vegetation and the new vegetation based on discrimination between C3 and C4 plants. The present study aimed to assessing the impact of difference land-use systems on C3 and C4 contribution to SOC. The experimental area is located inside the Cerrado biome. Soil samples were taken from six different land-use sites: (i) native local forest; (ii) Eucalyptus forest (EF) established in 1985 (OEC); (iii) EF established in 2004 (NEC); (iv) pasture of B. decumbens; (v) Agroforestry System (AF) established on 1994 (OAF); and (vi) AF established on 2004 (NAF). The establishment on AF was placed first with the eucalyptus planted and rice (Oryza sativa), soybean (Glycine max) and braquiaria grass (B. Brizantha cv. Marandu) in between trees rows. Soil was collected from four depths (0-10; 10-20; 20-50 and 50-100 cm). For stable C isotope analysis, whole soil was analyzed mass spectrometer. The percentage of SOC derived from the Brachiaria ssp., a C4 plant, or from the eucalyptus or native forest, a C3 plant, was estimated based on the equations: % C4-derived SOC = (?- ?T)/(?G- ?T) x 100 (1); % C3-derived SOC = 100 - % C4-derived SOC (2) Based on the equations... Mostrar Tudo |
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Palavras-Chave: |
Agroforestry systems; Brasil; Minas Gerais; Organic carbon; Sistema agroflorestal. |
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Thesagro: |
Carbono; Cerrado; Composto orgânico; Solo; Uso da terra. |
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Thesaurus NAL: |
Brazil; Land use; Organic compounds; Soil. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129065/1/cnpc-2015-Trees.pdf
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Marc: |
LEADER 02857nam a2200301 a 4500
001 2023312
005 2024-01-02
008 2015 bl uuuu u00u1 u #d
100 1 $aTONUCCI, R. G.
245 $aTrees and grass contribution to soil organic carbon in agroforestry systems.$h[electronic resource]
260 $aIn: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa$c2015
520 $aAgroforestry systems have the potential to enhance carbon (C) sequestration in soil compared with treeless (agricultural) systems (Montagnini & Nair, 2004). When one type of vegetation is replaced with another, stable isotope contents (?13 C) values can be used to identify soil organic carbon (SOC) derived from residues in the native vegetation and the new vegetation based on discrimination between C3 and C4 plants. The present study aimed to assessing the impact of difference land-use systems on C3 and C4 contribution to SOC. The experimental area is located inside the Cerrado biome. Soil samples were taken from six different land-use sites: (i) native local forest; (ii) Eucalyptus forest (EF) established in 1985 (OEC); (iii) EF established in 2004 (NEC); (iv) pasture of B. decumbens; (v) Agroforestry System (AF) established on 1994 (OAF); and (vi) AF established on 2004 (NAF). The establishment on AF was placed first with the eucalyptus planted and rice (Oryza sativa), soybean (Glycine max) and braquiaria grass (B. Brizantha cv. Marandu) in between trees rows. Soil was collected from four depths (0-10; 10-20; 20-50 and 50-100 cm). For stable C isotope analysis, whole soil was analyzed mass spectrometer. The percentage of SOC derived from the Brachiaria ssp., a C4 plant, or from the eucalyptus or native forest, a C3 plant, was estimated based on the equations: % C4-derived SOC = (?- ?T)/(?G- ?T) x 100 (1); % C3-derived SOC = 100 - % C4-derived SOC (2) Based on the equations 1 and 2 were calculated the contributions of each C3 and C4 species in SOC C-derived, as follows: C3-devived SOC (Mg ha-1) = (% C3-derived SOC) x (SOC content, Mg ha-1) (3); C4-derived SOC (Mg ha-1) = (% C4-derived SOC) x (SOC content, Mg ha-1) (4). A complete randomized design was used with Tukey?s studentized. Statistical differences were considered significant at p <0.05.
650 $aBrazil
650 $aLand use
650 $aOrganic compounds
650 $aSoil
650 $aCarbono
650 $aCerrado
650 $aComposto orgânico
650 $aSolo
650 $aUso da terra
653 $aAgroforestry systems
653 $aBrasil
653 $aMinas Gerais
653 $aOrganic carbon
653 $aSistema agroflorestal
700 1 $aGARCIA, R.
700 1 $aNAIR, V.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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