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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/02/2017 |
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Data da última atualização: |
20/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIRARDINI, L. K.; PAIM, D. S.; AUSANI, THAIS C.; LOPES, G. V.; PELLEGRINI, D. C. P.; BRITO, M. A. V. P. e; CARDOSO, M. |
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Afiliação: |
Lilian K. Girardini, UNIV. OESTE DANTA CATARINA; Daniel S. Paim, UFRGS; Thais C. Ausani, UFRGS; Graciela V. Lopes, UFRGS; Debora C.P. Pellegrini, UNIV. FEDERAL DO PAMPA; MARIA APARECIDA V PAIVA E BRITO, CNPGL; Marisa Cardoso, UFRGS. |
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Título: |
Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 36, n. 10, p. 951-956, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance
phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin
(18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates
belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters
can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials. MenosIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance
phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin
(18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates
belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused b... Mostrar Tudo |
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Palavras-Chave: |
MRSA; PFGE clusters. |
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Thesaurus Nal: |
mastitis; methicillin-resistant Staphylococcus aureus. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/156043/1/Cnpgl-2016-PesqVetBras-Antimicrobial.pdf
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Marc: |
LEADER 02388naa a2200241 a 4500
001 2064660
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008 2016 bl --- 0-- u #d
100 1 $aGIRARDINI, L. K.
245 $aAntimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil.
260 $c2016
520 $aIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.
650 $amastitis
650 $amethicillin-resistant Staphylococcus aureus
653 $aMRSA
653 $aPFGE clusters
700 1 $aPAIM, D. S.
700 1 $aAUSANI, THAIS C.
700 1 $aLOPES, G. V.
700 1 $aPELLEGRINI, D. C. P.
700 1 $aBRITO, M. A. V. P. e
700 1 $aCARDOSO, M.
773 $tPesquisa Veterinária Brasileira$gv. 36, n. 10, p. 951-956, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 8 | |
| 2. |  | GIRARDINI, L. K.; PAIM, D. S.; LOPES, G. V.; PELLEGRINI, D. C. P.; BRITO, M. A. V. P.; CARDOSO, M. Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 10, p. 951-956, out. 2016.| Biblioteca(s): Área de Informação da Sede. |
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| 3. | | WERLANG, G. O.; PAIM, D. S; VIEIRA, T. R.; PISSETTI, C.; KICH, J. D.; CARDOSO, M. R. de I. Detection of salmonella heidelberg resistant to colistin in the intestinal content of pigs at slaughter. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF BIOLOGICAL, CHEMICAL AND PHYSICAL HAZARDS IN PIG AND PORK, 12., 2017, Foz do Iguaçu. Proceedings Book. Concórdia: Embrapa Suínos e Aves, 2017. p. 175-179. SafePork 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | PELLEGRINI, D. C. P.; PAIM, D. S.; LIMA, G. J. M. M. de; KICH, J. D.; COLDEBELLA, A.; CARDOSO, M. Frequência de isolamento de salmonella sp e de enterobactérias em diferentes áreas de fábricas de ração para suínos In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 14., 2009, Uberlândia. Anais... Uberlândia: Abraves, 2009. p. 359-360. 1 CD-ROM. Subprojeto: 02.06.60.400-06| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | GIRARDINI, L. K.; PAIM, D. S.; AUSANI, THAIS C.; LOPES, G. V.; PELLEGRINI, D. C. P.; BRITO, M. A. V. P. e; CARDOSO, M. Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil. Pesquisa Veterinária Brasileira, v. 36, n. 10, p. 951-956, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | SANTIAGO-NETO, W.; MACHADO, G.; PAIM, D. S.; CAMPOS, T. de; BRITO, M. A. V. P. e; CARDOSO, M. R. I.; CORBELLINI, L. G. Relação da idade na presença de bactérias resistentes a antimicrobianos em rebanhos leiteiros no Rio Grande do Sul. Pesquisa Veterinária Brasileira, v. 34, n. 7, p. 613-620, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | PAIM, D. S.; PELLEGRINI, D. da C. P.; CARDOSO, M. R. de I; LIMA, G. J. M. M. de; KICH, J. D.; COLDEBELLA, A. Frequência de isolamento de Salmonella sp. e de enterobactérias em fábricas de ração para suínos. In: CONGRESSO SUL BRASILEIRO DE AVICULTURA, SUINOCULTURA E LATICINIOS, 2., Bento Gonçalves, 2010. Feira de equipamentos, serviços e tecnologias: resumos... Bento Gonçalves: AVISULAT, 2010. Disponível em:. Acesso em: 16 dez 2010. Projeto/Plano de Ação: 16.00.30.004-00.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | PAIM, D. S.; PISSETTI, C.; VIEIRA, T. R.; WERLANG, G. O.; COSTA, E. de F.; KICH, J. D.; CARDOSO, M. R. de I. Enumeration, antimicrobial resistance and typing of salmonella enterica: profile of strains carried in the intestinal contents of pigs at slaughter in southern Brazil. Acta Scientiae Veterinariae, v. 47, ed. 1636, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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