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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Unidades Centrais. |
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Data corrente: |
30/01/2017 |
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Data da última atualização: |
07/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANCHES, C. C.; ROSINHA, G. M. S.; GALVÃO, C. E.; FEIJO, G. L. D.; ARAUJO, F. R.; SOARES, C. O. |
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Afiliação: |
CRISTIANE C. SANCHES, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEER E. GALVÃO, UFMS; GELSON LUIS DIAS FEIJO, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC. |
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Título: |
Polymorphisms in the prion protein gene of cattle breeds from Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 11, p. 1059-1066, nov. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
One of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms. |
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Palavras-Chave: |
Indel; PRNP. |
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Thesagro: |
Bovino; Doença da vaca louca; Polimorfismo. |
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Thesaurus Nal: |
Bovine spongiform encephalopathy; Brazil; Cattle; Prions. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154345/1/Polymorphisms-in-the-prion.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154252/1/Polymorphisms-in-the-prion.pdf
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Marc: |
LEADER 02059naa a2200289 a 4500
001 2062269
005 2017-03-07
008 2016 bl uuuu u00u1 u #d
100 1 $aSANCHES, C. C.
245 $aPolymorphisms in the prion protein gene of cattle breeds from Brazil.$h[electronic resource]
260 $c2016
520 $aOne of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.
650 $aBovine spongiform encephalopathy
650 $aBrazil
650 $aCattle
650 $aPrions
650 $aBovino
650 $aDoença da vaca louca
650 $aPolimorfismo
653 $aIndel
653 $aPRNP
700 1 $aROSINHA, G. M. S.
700 1 $aGALVÃO, C. E.
700 1 $aFEIJO, G. L. D.
700 1 $aARAUJO, F. R.
700 1 $aSOARES, C. O.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 36, n. 11, p. 1059-1066, nov. 2016.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Biblioteca |
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Origem |
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Cutter |
Registro |
Volume |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
18/08/2008 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Nacional - A |
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Autoria: |
MATOS, W. O.; NÓBREGA, J. de A.; SOUZA, G. B. de; NOGUEIRA, A. R. de A. |
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Afiliação: |
Wladiana Oliveira Matos, UFSCar; Joaquim de Araújo Nóbrega, USFCar/SÃO CARLOS, SP.; GILBERTO BATISTA DE SOUZA, CPPSE; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
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Título: |
Especiação redox de cromo em solo acidentalmente contaminado com solução sulfocrômica. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Química Nova, v. 31, n. 6, p. 1450-1454, 2008. |
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DOI: |
10.1590/S0100-40422008000600032 |
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Idioma: |
Português |
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Conteúdo: |
Determination of Cr(VI) and Cr(III) was studied in soil samples accidentally contaminated with sulphochromic solution. Molecular absorption spectrophotometry based on the diphenylcarbazide method was used for the determination of Cr(VI) after its alkaline extraction. The total chromium concentration was determined using ICP OES. The quantification of Cr(III) was accomplished by subtracting the Cr(VI) concentration from the total chromium concentration. Regardless of the known contamination of the soil samples by sulphochromic solution, concentrations of Cr(VI) were below the detection limit. Addition and recovery experiments for Cr(VI) in soil samples with and without organic matter indicated its influence on the reduction of Cr(VI) to Cr(III). |
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Palavras-Chave: |
Chromium Redox; Speciation. |
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Thesagro: |
Solo. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40871/1/PROCIGBS2008.00050.pdf
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Marc: |
LEADER 01375naa a2200205 a 4500
001 1048502
005 2023-03-07
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-40422008000600032$2DOI
100 1 $aMATOS, W. O.
245 $aEspeciação redox de cromo em solo acidentalmente contaminado com solução sulfocrômica.$h[electronic resource]
260 $c2008
520 $aDetermination of Cr(VI) and Cr(III) was studied in soil samples accidentally contaminated with sulphochromic solution. Molecular absorption spectrophotometry based on the diphenylcarbazide method was used for the determination of Cr(VI) after its alkaline extraction. The total chromium concentration was determined using ICP OES. The quantification of Cr(III) was accomplished by subtracting the Cr(VI) concentration from the total chromium concentration. Regardless of the known contamination of the soil samples by sulphochromic solution, concentrations of Cr(VI) were below the detection limit. Addition and recovery experiments for Cr(VI) in soil samples with and without organic matter indicated its influence on the reduction of Cr(VI) to Cr(III).
650 $aSolo
653 $aChromium Redox
653 $aSpeciation
700 1 $aNÓBREGA, J. de A.
700 1 $aSOUZA, G. B. de
700 1 $aNOGUEIRA, A. R. de A.
773 $tQuímica Nova$gv. 31, n. 6, p. 1450-1454, 2008.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
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