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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
19/09/2016 |
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Data da última atualização: |
22/09/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIS, A. K.; SILVA, A. P. da; SOUZA, J. C. de; TEIXEIRA, S. L.; RIBEIRO, J. M.; PEIXOTO, A. R.; PAZ, C. D. da. |
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Afiliação: |
ANA KAROLINA PAIS, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; ANA PAULA DA SILVA, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; JOSELITA CARDOSO DE SOUZA, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; SILVIO LOPES TEIXEIRA; JULIANA MARTINS RIBEIRO, CPATSA; ANA ROSA PEIXOTO, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB; CRISTIANE DOMINGOS DA PAZ, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia – UNEB. |
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Título: |
Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
African Journal of Biotechnology, v. 15, n. 36, p. 1995-1998, sept. 2016 |
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ISSN: |
1684-5315 |
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DOI: |
10.5897/AJB2016.15405 |
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Idioma: |
Inglês |
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Conteúdo: |
Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro. MenosMicropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of n... Mostrar Tudo |
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Palavras-Chave: |
Autoclavagem; Chemical sterilization; Contamination; Esterilização química; Hipoclorito de sódio; In vitro; NaOC. |
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Thesagro: |
Contaminação; Cultura de tecido; Gerbera; Micropropagação; Planta ornamental. |
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Thesaurus Nal: |
Sodium hypochlorite; Tissue culture. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147573/1/Juliana-2016.pdf
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Marc: |
LEADER 02671naa a2200385 a 4500
001 2053027
005 2016-09-22
008 2016 bl uuuu u00u1 u #d
022 $a1684-5315
024 7 $a10.5897/AJB2016.15405$2DOI
100 1 $aPAIS, A. K.
245 $aSodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre.$h[electronic resource]
260 $c2016
520 $aMicropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro.
650 $aSodium hypochlorite
650 $aTissue culture
650 $aContaminação
650 $aCultura de tecido
650 $aGerbera
650 $aMicropropagação
650 $aPlanta ornamental
653 $aAutoclavagem
653 $aChemical sterilization
653 $aContamination
653 $aEsterilização química
653 $aHipoclorito de sódio
653 $aIn vitro
653 $aNaOC
700 1 $aSILVA, A. P. da
700 1 $aSOUZA, J. C. de
700 1 $aTEIXEIRA, S. L.
700 1 $aRIBEIRO, J. M.
700 1 $aPEIXOTO, A. R.
700 1 $aPAZ, C. D. da
773 $tAfrican Journal of Biotechnology$gv. 15, n. 36, p. 1995-1998, sept. 2016
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Embrapa Semiárido (CPATSA) |
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| 1. |  | REIS, J. C. L. 5ª These: Comentários. In: OTERO, D. T.; HADLER, E. C. (Org.). Actas: a classe rural resgatando as raízes da sua história. Pelotas, Editora Textos, 2008. p. 193-198.| Tipo: Capítulo em Livro Técnico-Científico |
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