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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
30/03/2016 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H. |
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Afiliação: |
Lucas Ferraz dos Santos, UESC/CENARGEN; Roberta Moreira Fregapani, CENARGEN; LOENI LUDKE FALCAO, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; MARCOS MOTA DO CARMO COSTA, CENARGEN; Uilson Vanderlei Lopes, CEPLAC/CEPEC; Karina Peres Gramacho, CEPLAC/CEPEC; RAFAEL MOYSES ALVES, CPATU; Fabienne Micheli, UESC/CIRAD; LUCILIA HELENA MARCELLINO, CENARGEN. |
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Título: |
First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
PLoS ONE, v. 11, n. 3, e0151074, 2016. |
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DOI: |
10.1371/journal.pone.0151074 |
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Idioma: |
Inglês |
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Conteúdo: |
The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively. MenosThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cup... Mostrar Tudo |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Cacau; Cupuaçu; Fruta Tropical. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141829/1/2016Santos.pdf
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Marc: |
LEADER 02580naa a2200289 a 4500
001 2052633
005 2023-03-24
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0151074$2DOI
100 1 $aSANTOS, L. F. dos
245 $aFirst microsatellite markers developed from cupuassu ESTs$bapplication in diversity analysis and cross-species transferability to cacao.$h[electronic resource]
260 $c2016
520 $aThe cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively. From the 1,517 ESTs containing SSRs, 70 ESTs were selected based on their functional annotation, focusing on pulp and seed quality, as well as resistance to pathogens. The 70 ESTs selected contained 77 SSRs, and among which, 11 were polymorphic in cupuassu genotypes. These EST-SSRs were able to discriminate the cupuassu genotype in relation to resistance/susceptibility to witches? broom disease, as well as to pulp quality (SST/ATT values). Finally, we showed that these markers were transferable to cacao genotypes, and that genome availability might be used as a predictive tool for polymorphism detection and primer design useful for both Theobroma species. To our knowledge, this is the first report involving EST-SSRs from cupuassu and is also a pioneer in the analysis of marker transferability from cupuassu to cacao. Moreover, these markers might contribute to develop or saturate the cupuassu and cacao genetic maps, respectively.
650 $aCacau
650 $aCupuaçu
650 $aFruta Tropical
653 $aMelhoramento genético
700 1 $aFREGAPANI, R. M.
700 1 $aFALCAO, L. L.
700 1 $aTOGAWA, R. C.
700 1 $aCOSTA, M. M. do C.
700 1 $aLOPES, U. V.
700 1 $aGRAMACHO, K. P.
700 1 $aALVES, R. M.
700 1 $aMICHELI, F.
700 1 $aMARCELLINO, L. H.
773 $tPLoS ONE$gv. 11, n. 3, e0151074, 2016.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 1. |  | SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H. First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao. PLoS ONE, v. 11, n. 3, e0151074, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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