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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
28/07/2016 |
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Data da última atualização: |
08/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENEZES, K. A. S.; NUNES, G. F. de O.; SAMPAIO, A. A.; SILVA, A. F. da; SOUZA, L. S. B. de; GAVA, C. A. T.; MARTINS, L. M. V.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
KELLY ALEXSANDRA SOUZA MENEZES, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; GERSIKA FAKIRRA DE OLIVEIRA NUNES, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; ALINE ARAUJO SAMPAIO, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; ALEKSANDRO FERREIRA DA SILVA, Universidade Federal Rural de Pernambuco; Recife, PE; LAYANE SILVA BARBOSA DE SOUZA, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; CARLOS ALBERTO TUAO GAVA, CPATSA; LINDETE MÍRIA VIEIRA MARTINS, Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Juazeiro, BA; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista de Ciências Agrárias, Lisboa, v. 39, n. 2, p. 222-233, 2016. |
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DOI: |
10.19084/RCA15050 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aims to evaluate the phenotypical characteristics of bacterial isolates from mulungu (Erythrina velutina Willd.) nodules and determinate their Box-PCR fingerprinting. All bacteria were evaluated by the following phenotypic characteristics: growth rate, pH change, colony color and mucus production. The bacterial isolates able to re-nodulate the original host were also evaluated regarding its tolerance to increased salinity and different incubation temperatures, ability to growth using different carbon sources, intrinsic antibiotic resistance and ?in vitro? auxin biosynthesis. The molecular fingerprints were set up using the Box-PCR technique and the isolates were clustered by their profiles. Among the 22 bacterial isolates obtained, eight were able to re-nodulate the original host. Among the nodule inducing isolates, some were tolerant to 1% of NaCl and 39° C and all of them metabolized the maltose, fructose, glucose, sucrose and arabinose, were resistant to rifampicin and produced auxin. The bacteria showed low genetic similarity among them and reference strains, which indicates the great genetic variability of the isolates. The results of this work are the first reports about the bacterial isolates able to nodulate this species. A more deep study of these bacteria may reveal the existence of isolates tolerant to environmental stresses and suitable as a future mulungu inoculant. |
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Palavras-Chave: |
Bactérias isoladas de nódulos; Biological nitrogen fixation; Box-PCR; Fixação biológica de azoto; Inoculant; Leguminosa arbórea; Planta nativa; Rhizobia; Rizóbio; Tree legumes. |
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Thesagro: |
Caatinga; Inoculante; Mulungu. |
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Thesaurus Nal: |
Erythrina velutina. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145867/1/Paulo-Ivan-1.pdf
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Marc: |
LEADER 02595naa a2200385 a 4500
001 2049759
005 2017-02-08
008 2016 bl uuuu u00u1 u #d
024 7 $a10.19084/RCA15050$2DOI
100 1 $aMENEZES, K. A. S.
245 $aDiversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil).$h[electronic resource]
260 $c2016
520 $aThis study aims to evaluate the phenotypical characteristics of bacterial isolates from mulungu (Erythrina velutina Willd.) nodules and determinate their Box-PCR fingerprinting. All bacteria were evaluated by the following phenotypic characteristics: growth rate, pH change, colony color and mucus production. The bacterial isolates able to re-nodulate the original host were also evaluated regarding its tolerance to increased salinity and different incubation temperatures, ability to growth using different carbon sources, intrinsic antibiotic resistance and ?in vitro? auxin biosynthesis. The molecular fingerprints were set up using the Box-PCR technique and the isolates were clustered by their profiles. Among the 22 bacterial isolates obtained, eight were able to re-nodulate the original host. Among the nodule inducing isolates, some were tolerant to 1% of NaCl and 39° C and all of them metabolized the maltose, fructose, glucose, sucrose and arabinose, were resistant to rifampicin and produced auxin. The bacteria showed low genetic similarity among them and reference strains, which indicates the great genetic variability of the isolates. The results of this work are the first reports about the bacterial isolates able to nodulate this species. A more deep study of these bacteria may reveal the existence of isolates tolerant to environmental stresses and suitable as a future mulungu inoculant.
650 $aErythrina velutina
650 $aCaatinga
650 $aInoculante
650 $aMulungu
653 $aBactérias isoladas de nódulos
653 $aBiological nitrogen fixation
653 $aBox-PCR
653 $aFixação biológica de azoto
653 $aInoculant
653 $aLeguminosa arbórea
653 $aPlanta nativa
653 $aRhizobia
653 $aRizóbio
653 $aTree legumes
700 1 $aNUNES, G. F. de O.
700 1 $aSAMPAIO, A. A.
700 1 $aSILVA, A. F. da
700 1 $aSOUZA, L. S. B. de
700 1 $aGAVA, C. A. T.
700 1 $aMARTINS, L. M. V.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tRevista de Ciências Agrárias, Lisboa$gv. 39, n. 2, p. 222-233, 2016.
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Embrapa Semiárido (CPATSA) |
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| 1. |  | MENEZES, K. A. S.; NUNES, G. F. de O.; SAMPAIO, A. A.; SILVA, A. F. da; SOUZA, L. S. B. de; GAVA, C. A. T.; MARTINS, L. M. V.; FERNANDES JUNIOR, P. I. Diversity of new root nodule bacteria from Erythrina velutina Willd., a native legume from the Caatinga dry forest (Northeastern Brazil). Revista de Ciências Agrárias, Lisboa, v. 39, n. 2, p. 222-233, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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