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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
30/06/2016 |
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Data da última atualização: |
30/06/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RIOS, E.; RESENDE, M.; KIRST, M.; RESENDE, M. D. V. de; ALMEIDA FILHO, J. E. de; MUNOZ, P. |
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Afiliação: |
Esteban Rios, University of Florida; Marcio Resende, RAPiD Genomics LLC; Matias Kirst, UFPR; MARCOS DEON VILELA DE RESENDE, CNPF; Janeo E. de Almeida Filho, UFV; Patricio Munoz, UFPR. |
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Título: |
Predictive ability of Genomic Estimated Family Values (GEFV). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego. [Abstracts...]. San Diego: [s.n.], 2016. Pôster P1186. |
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Idioma: |
Inglês |
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Conteúdo: |
Genomic selection (GS) has been used to compute genomic estimated breeding values (GEBV) of individuals; however, it has only been applied to animal and major plant crops due to high costs. Besides, breeding and selection is performed at the family level in some crops. We aimed to study the implementation of genome-wide family selection (GWFS) in two loblolly pine (Pinus taeda L.) populations: i) the breeding population CCLONES composed of 63 families (5-20 individuals per family), phenotyped for four traits (stem diameter, stem rust susceptibility, tree stiffness and lignin content) and genotyped using an Illumina Infinium assay with 4740 polymorphic SNPs, and ii) a simulated population that reproduced the same pedigree as CCLONES, 5000 polymorphic loci and two traits (oligogenic and polygenic). In both populations, phenotypic and genotypic data was pooled at the family level in silico. Phenotypes were averaged across replicates for all the individuals and allele frequency was computed for each SNP. Marker effects were estimated at the individual (GEBV) and family (GEFV) levels with Bayes-B using the package BGLR in R and models were validated using 10-fold cross validations. Predicted ability, computed by correlating phenotypes with GEBV and GEFV, was always higher for GEFV in both populations, even after standardizing GEFV predictions to be comparable to GEBV. Results revealed great potential for using GWFS in breeding programs that select families, such as most outbreeding forage species. A significant drop in genotyping costs as one sample per family is needed would allow the application of GWFS in minor crops. MenosGenomic selection (GS) has been used to compute genomic estimated breeding values (GEBV) of individuals; however, it has only been applied to animal and major plant crops due to high costs. Besides, breeding and selection is performed at the family level in some crops. We aimed to study the implementation of genome-wide family selection (GWFS) in two loblolly pine (Pinus taeda L.) populations: i) the breeding population CCLONES composed of 63 families (5-20 individuals per family), phenotyped for four traits (stem diameter, stem rust susceptibility, tree stiffness and lignin content) and genotyped using an Illumina Infinium assay with 4740 polymorphic SNPs, and ii) a simulated population that reproduced the same pedigree as CCLONES, 5000 polymorphic loci and two traits (oligogenic and polygenic). In both populations, phenotypic and genotypic data was pooled at the family level in silico. Phenotypes were averaged across replicates for all the individuals and allele frequency was computed for each SNP. Marker effects were estimated at the individual (GEBV) and family (GEFV) levels with Bayes-B using the package BGLR in R and models were validated using 10-fold cross validations. Predicted ability, computed by correlating phenotypes with GEBV and GEFV, was always higher for GEFV in both populations, even after standardizing GEFV predictions to be comparable to GEBV. Results revealed great potential for using GWFS in breeding programs that select families, such as most outbreedi... Mostrar Tudo |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Melhoramento vegetal; Pinus Taeda. |
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Thesaurus Nal: |
Plant breeding. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144954/1/2016-M.Deon-IPAG-Predicty-Ability.pdf
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Marc: |
LEADER 02338nam a2200217 a 4500
001 2048088
005 2016-06-30
008 2016 bl uuuu u00u1 u #d
100 1 $aRIOS, E.
245 $aPredictive ability of Genomic Estimated Family Values (GEFV).$h[electronic resource]
260 $aIn: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego. [Abstracts...]. San Diego: [s.n.], 2016. Pôster P1186.$c1186
520 $aGenomic selection (GS) has been used to compute genomic estimated breeding values (GEBV) of individuals; however, it has only been applied to animal and major plant crops due to high costs. Besides, breeding and selection is performed at the family level in some crops. We aimed to study the implementation of genome-wide family selection (GWFS) in two loblolly pine (Pinus taeda L.) populations: i) the breeding population CCLONES composed of 63 families (5-20 individuals per family), phenotyped for four traits (stem diameter, stem rust susceptibility, tree stiffness and lignin content) and genotyped using an Illumina Infinium assay with 4740 polymorphic SNPs, and ii) a simulated population that reproduced the same pedigree as CCLONES, 5000 polymorphic loci and two traits (oligogenic and polygenic). In both populations, phenotypic and genotypic data was pooled at the family level in silico. Phenotypes were averaged across replicates for all the individuals and allele frequency was computed for each SNP. Marker effects were estimated at the individual (GEBV) and family (GEFV) levels with Bayes-B using the package BGLR in R and models were validated using 10-fold cross validations. Predicted ability, computed by correlating phenotypes with GEBV and GEFV, was always higher for GEFV in both populations, even after standardizing GEFV predictions to be comparable to GEBV. Results revealed great potential for using GWFS in breeding programs that select families, such as most outbreeding forage species. A significant drop in genotyping costs as one sample per family is needed would allow the application of GWFS in minor crops.
650 $aPlant breeding
650 $aMelhoramento vegetal
650 $aPinus Taeda
653 $aMelhoramento genético
700 1 $aRESENDE, M.
700 1 $aKIRST, M.
700 1 $aRESENDE, M. D. V. de
700 1 $aALMEIDA FILHO, J. E. de
700 1 $aMUNOZ, P.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
25/11/2022 |
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Data da última atualização: |
11/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
VILELA, A. L. O.; ROSA, S. D. V. F. da; COELHO, S. V. B.; PEREIRA, C. C.; SOUZA, A. C. de; RIBEIRO, F. A. S. |
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Afiliação: |
ANA LUIZA OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO PEREIRA, UNIVERSIDADE FEDERAL DE LAVRAS; ANA CRISTINA DE SOUZA, UNIVERSIDADE FEDERAL DE LAVRAS; FERNANDO AUGUSTO SALES RIBEIRO, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Acclimatization of coffee seedlings obtained from zygotic embryos of aged seeds. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 16, n. 9, p.112-1134, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability. MenosCoffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, ... Mostrar Tudo |
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Palavras-Chave: |
Zygotic embryos. |
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Thesaurus NAL: |
Coffea arabica var. arabica; Enzyme substrates; Greenhouses; In vitro culture; Seedlings; Seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150877/1/Acclimatization-of-coffee-seedlings-obtained.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148763/1/Acclimatization-of-coffee-seedlings-obtained.pdf
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Marc: |
LEADER 02455naa a2200265 a 4500
001 2150877
005 2023-01-11
008 2022 bl uuuu u00u1 u #d
100 1 $aVILELA, A. L. O.
245 $aAcclimatization of coffee seedlings obtained from zygotic embryos of aged seeds.$h[electronic resource]
260 $c2022
520 $aCoffee seeds rapidly lose viability during storage, which hinders the development of vigorous seedlings for crop establishment. There are reports that seed endosperm is more sensitive to deterioration than embryos, which can be excised and cultivated in vitro. However, a substantial number of plants grown in vitro do not survive during transfer to a greenhouse or field environment. The objective of this study was to evaluate the acclimatization of coffee seedlings of cultivar Catuaí Amarelo IAC 62, developed from zygotic embryos obtained from aged seeds in different substrates and environments, for the production of well-developed seedlings suitable for planting. For this purpose, seedlings were obtained from the in vitro cultivation of embryos obtained from seeds of two quality levels: freshly harvested seeds and artificially aged seeds. Zygotic embryos were extracted from the seeds and cultivated in MS medium. At 60 days, the percentages of normal and abnormal seedlings and dead seeds were evaluated. The good-quality seedlings grown in vitro for 60 days were transplanted into two different substrates (Tropstrato and coconut fiber) and acclimatized in two environments (growth room and greenhouse with a misting system). The plants were evaluated for height, stem diameter, number of leaves, chlorophyll content, and growth rate. The greenhouse environment was better for seedling growth, possibly due to its higher sunlight and temperature. The best substrate was coconut fiber, as it ensured better development of plants from freshly harvested seeds and those from aged seeds. It is possible to develop healthy seedlings from seeds with low viability.
650 $aCoffea arabica var. arabica
650 $aEnzyme substrates
650 $aGreenhouses
650 $aIn vitro culture
650 $aSeedlings
650 $aSeeds
653 $aZygotic embryos
700 1 $aROSA, S. D. V. F. da
700 1 $aCOELHO, S. V. B.
700 1 $aPEREIRA, C. C.
700 1 $aSOUZA, A. C. de
700 1 $aRIBEIRO, F. A. S.
773 $tAustralian Journal of Crop Science$gv. 16, n. 9, p.112-1134, 2022.
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Embrapa Café (CNPCa) |
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