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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
16/06/2016 |
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Data da última atualização: |
09/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PÉRTILLE, F.; BOSAGNA, C. G.; SILVA, V. H. da; BOSCHIERO, C.; NUNES, J. de R. da S.; LEDUR, M. C.; JENSEN, P.; COUTINHO, L. L. |
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Afiliação: |
FÁBIO PÉRTILLE, ESALQ; CARLOS GUERRERO BOSAGNA, Linköping University; VINICIUS HENRIQUE DA SILVA, ESALQ; CLARISSA BOSCHIERO, ESALQ; JOSÉ DE RIBAMAR DA SILVA NUNES, ESALQ; MONICA CORREA LEDUR, CNPSA; PER JENSEN, Linköping University; LUIZ LEHMANN COUTINHO, ESALQ. |
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Título: |
High-throughput and cost-effective chicken genotyping using next-generation sequencing. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Scientific Reports, v. 6, n. 26929, 2016. |
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DOI: |
10.1038/srep26929 |
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Idioma: |
Inglês |
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Conteúdo: |
Chicken genotyping is becoming common practice in conventional animal breeding improvement. Despite the power of high-throughput methods for genotyping, their high cost limits large scale use in animal breeding and selection. In the present paper we optimized the CornellGBS, an efficient and costeffective genotyping by sequence approach developed in plants, for its application in chickens. Here we describe the successful genotyping of a large number of chickens (462) using CornellGBS approach. Genomic DNA was cleaved with the PstI enzyme, ligated to adapters with barcodes identifying individual animals, and then sequenced on Illumina platform. After filtering parameters were applied, 134,528 SNPs were identified in our experimental population of chickens. Of these SNPs, 67,096 had a minimum taxon call rate of 90% and were considered ?unique tags?. Interestingly, 20.7% of these unique tags have not been previously reported in the dbSNP. Moreover, 92.6% of these SNPs were concordant with a previous Whole Chicken-genome re-sequencing dataset used for validation purposes. The application of CornellGBS in chickens showed high performance to infer SNPs, particularly in exonic regions and microchromosomes. This approach represents a cost-effective (~US$50/sample) and powerful alternative to current genotyping methods, which has the potential to improve wholegenome selection (WGS), and genome-wide association studies (GWAS) in chicken production. |
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Thesagro: |
Avicultura; Melhoramento genético animal; Produção animal. |
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Thesaurus Nal: |
Animal breeding; Poultry production. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144504/1/final8219.pdf
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Marc: |
LEADER 02269naa a2200277 a 4500
001 2047245
005 2018-05-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1038/srep26929$2DOI
100 1 $aPÉRTILLE, F.
245 $aHigh-throughput and cost-effective chicken genotyping using next-generation sequencing.$h[electronic resource]
260 $c2016
520 $aChicken genotyping is becoming common practice in conventional animal breeding improvement. Despite the power of high-throughput methods for genotyping, their high cost limits large scale use in animal breeding and selection. In the present paper we optimized the CornellGBS, an efficient and costeffective genotyping by sequence approach developed in plants, for its application in chickens. Here we describe the successful genotyping of a large number of chickens (462) using CornellGBS approach. Genomic DNA was cleaved with the PstI enzyme, ligated to adapters with barcodes identifying individual animals, and then sequenced on Illumina platform. After filtering parameters were applied, 134,528 SNPs were identified in our experimental population of chickens. Of these SNPs, 67,096 had a minimum taxon call rate of 90% and were considered ?unique tags?. Interestingly, 20.7% of these unique tags have not been previously reported in the dbSNP. Moreover, 92.6% of these SNPs were concordant with a previous Whole Chicken-genome re-sequencing dataset used for validation purposes. The application of CornellGBS in chickens showed high performance to infer SNPs, particularly in exonic regions and microchromosomes. This approach represents a cost-effective (~US$50/sample) and powerful alternative to current genotyping methods, which has the potential to improve wholegenome selection (WGS), and genome-wide association studies (GWAS) in chicken production.
650 $aAnimal breeding
650 $aPoultry production
650 $aAvicultura
650 $aMelhoramento genético animal
650 $aProdução animal
700 1 $aBOSAGNA, C. G.
700 1 $aSILVA, V. H. da
700 1 $aBOSCHIERO, C.
700 1 $aNUNES, J. de R. da S.
700 1 $aLEDUR, M. C.
700 1 $aJENSEN, P.
700 1 $aCOUTINHO, L. L.
773 $tScientific Reports$gv. 6, n. 26929, 2016.
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Embrapa Suínos e Aves (CNPSA) |
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| 1. |  | PÉRTILLE, F.; BOSAGNA, C. G.; SILVA, V. H. da; BOSCHIERO, C.; NUNES, J. de R. da S.; LEDUR, M. C.; JENSEN, P.; COUTINHO, L. L. High-throughput and cost-effective chicken genotyping using next-generation sequencing. Scientific Reports, v. 6, n. 26929, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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