| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
14/03/2016 |
|
Data da última atualização: |
22/03/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FERRÃO, L. F. V.; CAIXETA, E. T.; PENA, G.; ZAMBOLIM, E. M.; CRUZ, C. D.; ZAMBOLIM, L.; FERRAO, M. A. G.; SAKIYAMA, N. S. |
|
Afiliação: |
LUÍS FELIPE V. FERRÃO, UFV; EVELINE TEIXEIRA CAIXETA, SAPC; GUILHERME PENA, UFV; EUNICE M. ZAMBOLIM, UFV; COMES D. CRUZ, UFV; LAÉRCIO ZAMBOLIM, UFV; MARIA AMELIA GAVA FERRAO, SAPC; NEY S. SAKIYAMA, UFV. |
|
Título: |
New EST-SSR markers of Coffea arabica: transferability and application to studies of molecular characterization and genetic mapping. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Molecular Breeding, v. 35, n. 1, p. 2-5, jan. 2015. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Microsatellite markers (SSR) have broad utility in genetic studies due to a high rate of polymor-phisms, a codominant nature and multiallelism. EST?SSRs are markers derived from the expressed sequences of a genome and represent transcribed genes. Despite the importance of the genus Coffea , only a small number of EST?SSR markers are currently available. Thus, this study was designed to mine and develop a new set of EST?SSR markers from the Brazilian Coffee Genome Project. We investigated 130,792 expressed sequence tags (ESTs), from which 24,031 DNA sequences with microsatellites were identified. After stability and amplification testing, 101 new EST?SSR markers were developed and analyzed in different coffee species. The average rate of transferability was 88 %, showing that these markers are useful in genetic studies across the genus Coffea. Polymorphism levels and the degree of diversity were consistent with the evolutionary history of the species. All coffee genotypes were discriminated, even the C. arabica genotypes that have known narrow genetic basis. It was also possible to locate 14 EST? SSRs into different linkage groups of the C. arabica genetic map, which demonstrate that these markers can be useful in QTL mapping studies and in molecular-assisted selection. |
|
Palavras-Chave: |
Brazilian Coffee Genome Project; Microsatellites. |
|
Thesagro: |
Coffea Arábica. |
|
Thesaurus Nal: |
Coffea; Genetic markers. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141110/1/New-EST-SSR-markers.pdf
|
|
Marc: |
LEADER 02100naa a2200265 a 4500
001 2040767
005 2016-03-22
008 2015 bl uuuu u00u1 u #d
100 1 $aFERRÃO, L. F. V.
245 $aNew EST-SSR markers of Coffea arabica$btransferability and application to studies of molecular characterization and genetic mapping.$h[electronic resource]
260 $c2015
520 $aMicrosatellite markers (SSR) have broad utility in genetic studies due to a high rate of polymor-phisms, a codominant nature and multiallelism. EST?SSRs are markers derived from the expressed sequences of a genome and represent transcribed genes. Despite the importance of the genus Coffea , only a small number of EST?SSR markers are currently available. Thus, this study was designed to mine and develop a new set of EST?SSR markers from the Brazilian Coffee Genome Project. We investigated 130,792 expressed sequence tags (ESTs), from which 24,031 DNA sequences with microsatellites were identified. After stability and amplification testing, 101 new EST?SSR markers were developed and analyzed in different coffee species. The average rate of transferability was 88 %, showing that these markers are useful in genetic studies across the genus Coffea. Polymorphism levels and the degree of diversity were consistent with the evolutionary history of the species. All coffee genotypes were discriminated, even the C. arabica genotypes that have known narrow genetic basis. It was also possible to locate 14 EST? SSRs into different linkage groups of the C. arabica genetic map, which demonstrate that these markers can be useful in QTL mapping studies and in molecular-assisted selection.
650 $aCoffea
650 $aGenetic markers
650 $aCoffea Arábica
653 $aBrazilian Coffee Genome Project
653 $aMicrosatellites
700 1 $aCAIXETA, E. T.
700 1 $aPENA, G.
700 1 $aZAMBOLIM, E. M.
700 1 $aCRUZ, C. D.
700 1 $aZAMBOLIM, L.
700 1 $aFERRAO, M. A. G.
700 1 $aSAKIYAMA, N. S.
773 $tMolecular Breeding$gv. 35, n. 1, p. 2-5, jan. 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | FERRÃO, L. F. V.; CAIXETA, E. T.; PENA, G.; ZAMBOLIM, E. M.; CRUZ, C. D.; ZAMBOLIM, L.; FERRAO, M. A. G.; SAKIYAMA, N. S. New EST-SSR markers of Coffea arabica: transferability and application to studies of molecular characterization and genetic mapping. Molecular Breeding, v. 35, n. 1, p. 2-5, jan. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
