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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TAKETANI, R. G.; MOITINHO, M. A.; MELO, I. S. de. |
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Afiliação: |
R. G. TAKETANI, Bolsista FAPESP; M. A. MOITINHO, Bolsista CNPq; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Metatranscriptomic and Metagenomic insights into the litter decomposition in mangroves. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1749-1. |
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Idioma: |
Português |
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Conteúdo: |
Mangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was a switch between Gamma and Deltaproteobacteria as dominant classes between mRNA and DNA (respectively). Another striking difference was that the DNA libraries were much more similar, despite the geographical distance than the mRNA. Hits findings highlight the importance of environmental signals to the expression of genes. MenosMangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was... Mostrar Tudo |
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Palavras-Chave: |
Mangrove; Metagenomic; Metatranscriptomic. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139532/1/2015RA-014.pdf
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Marc: |
LEADER 02441nam a2200169 a 4500
001 2037826
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aTAKETANI, R. G.
245 $aMetatranscriptomic and Metagenomic insights into the litter decomposition in mangroves.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1749-1.$c1749
520 $aMangroves are costal ecosystems known for its important role as sink and source of organic matter. This ecosystem covers most of the tropical and subtropical shores. A major portion of the organic matter that is produced by the mangrove vegetation is exported to nearby marine ecosystems. Therefore, this study was designed to shed light over the process of litter decomposition by using high throughput sequencing of the mRNA and DNA present in decaying mangrove leaves. With this purpose, litter bags contatining leaves of Laguncularia racemosa, Avicennia schaueriana and Rhizophora mangle were left on the mangrove sediment for 60 days. This experiment was conducted in three different mangroves in different locations. These material was had its total DNA and mRNA extracted and used to construct libraries that were sequenced in an ion torrent PGM. Sequences were annotated by MG-RAST, CAZY and Crass. The community patterns were very similar between plants; however, there were significant differences between locations. There were some important differences between the groups observed for in the DNA and mRNA libraries. The major phylogenetic groups observed were Gamma and Deltaproteobacteria while the functions were clustering-based subsystems, carbohydrate, amino acid and derivatives, protein, miscellaneous metabolism. The search for carbohydrate metabolism genes using CAZY found genes related to plant organic matter decomposition (GH26, GH17, AA3 and GH43). The major difference was a switch between Gamma and Deltaproteobacteria as dominant classes between mRNA and DNA (respectively). Another striking difference was that the DNA libraries were much more similar, despite the geographical distance than the mRNA. Hits findings highlight the importance of environmental signals to the expression of genes.
653 $aMangrove
653 $aMetagenomic
653 $aMetatranscriptomic
700 1 $aMOITINHO, M. A.
700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 12 | |
| 5. |  | SANTANA, F. A.; OLIVEIRA, L. A. de; GODOY, R. C. B. de; AMORIM, T. da S.; FONSECA, M. D. Influência do momento de adição do ácido cítrico no processamento de doce de banana em corte. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Potencialidades, desafios e inovações. Salvador: sbCTA, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 7. | | GODOY, R. C. B. de; WASZCZYNSKJ, N.; SANTANA, F. A.; SILVA, S. de O. e; SOUZA NETO, M. A.; OLIVEIRA, L. A. de; LEDO, C. A. da S. Banana cultivars with resistance to black leaf streak and potential for jam processing. Acta Horticulturae, n. 986, p. 309-317, 2013. Trabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. Eds.: I. Van den Bergh et al.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Florestas; Embrapa Mandioca e Fruticultura. |
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| 8. | | GODOY, R. C. B. de; BATISTA, D. V. S.; AZEREDO, H. M. C. de; OLIVEIRA, L. A. de; BENASSI, V. de T.; MANDARINO, J. M. G. Addition of passion fruit pulp as an alternative to enhance the flavor and nutritional value of a soybean beverage. In: PANGBORN SENSORY SCIENCE SYMPOSIUM, 10., 2013, Rio de Janeiro. Abstracts. [S.l.]: Rogil, 2013. Disponibilizado online mediante login e senha.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas; Embrapa Soja. |
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| 10. | | GODOY, R. C. B. de; WASZCZYNSKY, N.; SANTANA, F. A.; SILVA, S. de O. e; OLIVEIRA, L. A. de; SANTOS, G. G. dos. Physico-chemical characterization of banana varieties resistant to black leaf streak disease for industrial purposes. Ciência Rural, Santa Maria, v. 46, n. 9, p. 1514-1520, set. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas; Embrapa Mandioca e Fruticultura. |
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| 11. | | GODOY, R. C. B. de; WASZCZYNSKYJ, N.; SANTANA, F. A.; SILVA, S. de O.; SOUSA NETO, M. A. de; LEDO, C. A. da S.; OLIVEIRA, L. A. de. Varieties resistant to black leaf streak with the potential for jams processing. In: ISHS/PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [S.l.]: ISHS, 2011. p. 152-153.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas; Embrapa Mandioca e Fruticultura. |
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| 12. | | OLIVEIRA, E. J. de; RESENDE, M. D. V. de; SANTOS, V. da S.; FERREIRA, C. F.; OLIVEIRA, G. A. F.; SILVA, M. S. da; OLIVEIRA, L. A. de; AGUILAR-VILDOSO, C. I. Genome-wide selection in cassava. Euphytica, v.187, p. 263-276, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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