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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
26/11/2015 |
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Data da última atualização: |
26/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CRAVEIRO, S. R.; TOGAWA, R. C.; INGLIS, P. W.; GRYNBERG, P.; MELO, F. L.; RIBEIRO, Z. M. de A.; RIBEIRO, B. M.; BÁO, S. N.; CASTRO, M. E. B. de. |
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Afiliação: |
SALUANA R. CRAVEIRO; ROBERTO COITI TOGAWA, CENARGEN; PETER W. INGLIS; PRISCILA GRYMBERG, CENARGEN; FERNANDO L. MELO; ZILDA MARIA DE ARAUJO RIBEIRO, CENARGEN; BERGMANN M. RIBEIRO; SÔNIA N. BÁO; MARIA ELITA BATISTA DE CASTRO, CENARGEN. |
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Título: |
The genome sequence of Pseudoplusia includes single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC GENOMICS, jun., v.16, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. Results: The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX ? Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may be derived from an ancestor common to these viruses. The homologous repeats (hrs) are absent from the PsinSNPV genome, which is also the case in ChchNPV and TnSNPV. Two p26 gene homologs (p26a and p26b) were found in the PsinSNPV genome. P26 is thought to be required for optimal virion occlusion in the occlusion bodies (OBs), but its function is not well characterized. The P26 phylogenetic tree suggests that this gene was obtained from three independent acquisition events within the Baculoviridae family. The presence of a signal peptide only in the PsinSNPV p26a/ORF-20 homolog indicates distinct function between the two P26 proteins. Conclusions: PsinSNPV has a genomic sequence apparently different from other baculoviruses sequenced so far. The complete genome sequence of PsinSNPV will provide a valuable resource, contributing to studies on its molecular biology and functional genomics, and will promote the development of this virus as an effective bioinsecticide. MenosBackground: Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. Results: The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX ? Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may be derived from an ancestor common to these viruses. The homologous repeats (hrs) are absent from the PsinSNPV genome, which is also the case in ChchNPV and TnSNPV. Two p26 gene homologs (p26a and p26b) were found in the PsinSNPV ... Mostrar Tudo |
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Palavras-Chave: |
Insiticida biológico. |
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Thesagro: |
Baculovirus; Praga; Pseudoplusia Includens. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134081/1/s12864-015-1323-91.pdf
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Marc: |
LEADER 03035naa a2200265 a 4500
001 2029685
005 2024-04-26
008 2015 bl uuuu u00u1 u #d
100 1 $aCRAVEIRO, S. R.
245 $aThe genome sequence of Pseudoplusia includes single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses.$h[electronic resource]
260 $c2015
520 $aBackground: Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. Results: The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX ? Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may be derived from an ancestor common to these viruses. The homologous repeats (hrs) are absent from the PsinSNPV genome, which is also the case in ChchNPV and TnSNPV. Two p26 gene homologs (p26a and p26b) were found in the PsinSNPV genome. P26 is thought to be required for optimal virion occlusion in the occlusion bodies (OBs), but its function is not well characterized. The P26 phylogenetic tree suggests that this gene was obtained from three independent acquisition events within the Baculoviridae family. The presence of a signal peptide only in the PsinSNPV p26a/ORF-20 homolog indicates distinct function between the two P26 proteins. Conclusions: PsinSNPV has a genomic sequence apparently different from other baculoviruses sequenced so far. The complete genome sequence of PsinSNPV will provide a valuable resource, contributing to studies on its molecular biology and functional genomics, and will promote the development of this virus as an effective bioinsecticide.
650 $aBaculovirus
650 $aPraga
650 $aPseudoplusia Includens
653 $aInsiticida biológico
700 1 $aTOGAWA, R. C.
700 1 $aINGLIS, P. W.
700 1 $aGRYNBERG, P.
700 1 $aMELO, F. L.
700 1 $aRIBEIRO, Z. M. de A.
700 1 $aRIBEIRO, B. M.
700 1 $aBÁO, S. N.
700 1 $aCASTRO, M. E. B. de
773 $tBMC GENOMICS, jun.$gv.16, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
12/11/2015 |
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Data da última atualização: |
03/02/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MARINHO, N. P.; BOLZON de MUÑIZ, G. I.; NISGOSKI, S.; KLOCK, U.; ANDRADE, A. S. de; MAGALHAES, W. L. E. |
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Afiliação: |
NELSON P. MARINHO, Centro Universitário Autônomo do Brasil; GRACIELA I. BOLZON DE MUÑIZ, Universidade Federal do Paraná; SILVANA NISGOSKI, Universidade Federal do Paraná; UMBERTO KLOCK, Universidade Federal do Paraná; ALAN S. DE ANDRADE, Universidade Federal do Paraná; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF. |
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Título: |
Compósito à base de poli(hidroxibutirato) (PHB) reforçado com fibras de rami espécie Bohemeria nivea. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE POLÍMEROS, 13., 2015, Natal. Anais. [São Carlos: Associação Brasileira de Polímeros], 2015. |
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Páginas: |
5 p. |
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Descrição Física: |
Disponível online. |
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Idioma: |
Português |
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Conteúdo: |
Fibras de rami da espécie Bohemeria nivea foram usadas como elemento de reforço, nas proporções de 5%, 10% e 15%, para melhorar as propriedades mecânicas nos esforços de tração do poli(hidroxibutirato). Para obtenção das fibras foi utilizado o processo de polpação organosolv com uma taxa de lignina residual (kappa) de k=46,2. Nas diferentes etapas de branqueamento das fibras foram obtidos k=29,2 (1a etapa com ClO2); k=24,3 (2a etapa com H2O2); k=19,0 (3a etapa com ClO2) e k=7,6 (4a etapa com H2O2). Nos ensaios mecânicos de tração o Módulo de Elasticidade (MOE) apresentou baixos Coeficientes de Variação (CV) entre 12,45% e 17,35% e pouca dispersão no Desvio Padrão estando entre 50,41MPa e 65,93MPa para amostras de PHB/rami-15% e PHB/rami-5%, respectivamente. Na média o MOE ficou entre 404,86Mpa e 379,93MPa para PHB/rami-15%, e PHB/rami-5%, respectivamente. Com estes resultado é possível inferir que a adição de rami melhora as propriedades mecânicas do poli(hidroxibutirato). |
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Palavras-Chave: |
Bohemeria nivea; Composite; Compósito; Módulo de elasticidade; MOE; Organosolv; PolihidróxiButirato. |
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Thesagro: |
Rami. |
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Thesaurus NAL: |
polyhydroxybutyrate. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132917/1/2015-Washington-CBP-Composito.pdf
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Marc: |
LEADER 01941nam a2200289 a 4500
001 2028419
005 2016-02-03
008 2015 bl uuuu u00u1 u #d
100 1 $aMARINHO, N. P.
245 $aCompósito à base de poli(hidroxibutirato) (PHB) reforçado com fibras de rami espécie Bohemeria nivea.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE POLÍMEROS, 13., 2015, Natal. Anais. [São Carlos: Associação Brasileira de Polímeros]$c2015
300 $a5 p.$cDisponível online.
520 $aFibras de rami da espécie Bohemeria nivea foram usadas como elemento de reforço, nas proporções de 5%, 10% e 15%, para melhorar as propriedades mecânicas nos esforços de tração do poli(hidroxibutirato). Para obtenção das fibras foi utilizado o processo de polpação organosolv com uma taxa de lignina residual (kappa) de k=46,2. Nas diferentes etapas de branqueamento das fibras foram obtidos k=29,2 (1a etapa com ClO2); k=24,3 (2a etapa com H2O2); k=19,0 (3a etapa com ClO2) e k=7,6 (4a etapa com H2O2). Nos ensaios mecânicos de tração o Módulo de Elasticidade (MOE) apresentou baixos Coeficientes de Variação (CV) entre 12,45% e 17,35% e pouca dispersão no Desvio Padrão estando entre 50,41MPa e 65,93MPa para amostras de PHB/rami-15% e PHB/rami-5%, respectivamente. Na média o MOE ficou entre 404,86Mpa e 379,93MPa para PHB/rami-15%, e PHB/rami-5%, respectivamente. Com estes resultado é possível inferir que a adição de rami melhora as propriedades mecânicas do poli(hidroxibutirato).
650 $apolyhydroxybutyrate
650 $aRami
653 $aBohemeria nivea
653 $aComposite
653 $aCompósito
653 $aMódulo de elasticidade
653 $aMOE
653 $aOrganosolv
653 $aPolihidróxiButirato
700 1 $aBOLZON de MUÑIZ, G. I.
700 1 $aNISGOSKI, S.
700 1 $aKLOCK, U.
700 1 $aANDRADE, A. S. de
700 1 $aMAGALHAES, W. L. E.
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Embrapa Florestas (CNPF) |
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