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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/03/2015 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, A. S. de O. dos; COSTA, F. F.; ESTEVES, W. T.; BRITO, M. A. V. P. e; FURTADO, M. A. M.; MARTINS, M. F. |
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Afiliação: |
ALESSA SIQUEIRA DE OLIVEIRA DOS SANTOS; FABIANO FREIRE COSTA, UFJF; WESLEY TINOCO ESTEVES; MARIA APARECIDA V PAIVA E BRITO, CNPGL; MARCO ANTÔNIO MOREIRA FURTADO, UFJF; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Linearization of the Bradford protein assay to application in cow milk proteins quantification by UV-Vis spectrophotometry method. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Revista do Instituto de Laticínios Cândido Tostes, v. 69, n. 6, p. 415-423, 2014. |
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DOI: |
https://doi.org/10.14295/2238-6416.v69i6.350 |
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Idioma: |
Inglês |
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Conteúdo: |
Reliable methods for determination and quantification of total protein in food are essential information to ensure quality and safety of food trade. The objective of this study was to evaluate the linearity of calibration curves obtained from different proteins (blood serum albumin-BSA, α-LA, β-LG, caseins (CN): αs, β and κ-CAS) with the reagent of Bradford. Comercial UHT skimmed bovine milk was analyzed for the determination of total protein using the Bradford method by reading at 595 nm. The determination of the concentrations of total milk protein was achieved by linear regression. The Bradford method showed a high sensitivity for the determination of total proteins in bovine milk dilution 1:25 to values closer to those obtained by the Kjeldahl method. The results showed that the calibration curve of standard proteins β-CN and BSA obtained better linearity with less variation in the absorbance measurements for the determination of total protein of milk. |
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Palavras-Chave: |
Bovine; BSA; Proteins quantification; Skimmed milk; Spectrophotometry. |
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Thesaurus Nal: |
beta-casein. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/120712/1/Cnpgl-2014-RevILCT-Linearization.pdf
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Marc: |
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001 2011872
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024 7 $ahttps://doi.org/10.14295/2238-6416.v69i6.350$2DOI
100 1 $aSANTOS, A. S. de O. dos
245 $aLinearization of the Bradford protein assay to application in cow milk proteins quantification by UV-Vis spectrophotometry method.$h[electronic resource]
260 $c2014
520 $aReliable methods for determination and quantification of total protein in food are essential information to ensure quality and safety of food trade. The objective of this study was to evaluate the linearity of calibration curves obtained from different proteins (blood serum albumin-BSA, α-LA, β-LG, caseins (CN): αs, β and κ-CAS) with the reagent of Bradford. Comercial UHT skimmed bovine milk was analyzed for the determination of total protein using the Bradford method by reading at 595 nm. The determination of the concentrations of total milk protein was achieved by linear regression. The Bradford method showed a high sensitivity for the determination of total proteins in bovine milk dilution 1:25 to values closer to those obtained by the Kjeldahl method. The results showed that the calibration curve of standard proteins β-CN and BSA obtained better linearity with less variation in the absorbance measurements for the determination of total protein of milk.
650 $abeta-casein
653 $aBovine
653 $aBSA
653 $aProteins quantification
653 $aSkimmed milk
653 $aSpectrophotometry
700 1 $aCOSTA, F. F.
700 1 $aESTEVES, W. T.
700 1 $aBRITO, M. A. V. P. e
700 1 $aFURTADO, M. A. M.
700 1 $aMARTINS, M. F.
773 $tRevista do Instituto de Laticínios Cândido Tostes$gv. 69, n. 6, p. 415-423, 2014.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | SANTOS, A. S. de O. dos; COSTA, F. F.; ESTEVES, W. T.; BRITO, M. A. V. P. e; FURTADO, M. A. M.; MARTINS, M. F. Linearization of the Bradford protein assay to application in cow milk proteins quantification by UV-Vis spectrophotometry method. Revista do Instituto de Laticínios Cândido Tostes, v. 69, n. 6, p. 415-423, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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