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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
12/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A. |
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Afiliação: |
FURGS; FURGS; RODRIGO DA ROCHA FRAGOSO, CPAC; WAGNER ALEXANDRE LUCENA, CNPA; IAPAR; UNIVERSITY OF CALDAS, COLOMBIA; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, FRANCE; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN. |
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Título: |
Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Gene, v. 553, n. 1, p. 7-16, Dec. 2014. |
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DOI: |
10.1016/j.gene.2014.09.050 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies. MenosAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Amido; Curculionideo; Hidrolise; Praga de planta. |
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Thesaurus Nal: |
Curculionidae; Enzymatic hydrolysis; Gene expression; Insect pests; Starch. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114033/1/34279.pdf
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Marc: |
LEADER 02768naa a2200385 a 4500
001 2003023
005 2015-02-12
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.gene.2014.09.050$2DOI
100 1 $aBEZERRA, C. A.
245 $aMolecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei.
260 $c2014
520 $aAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies.
650 $aCurculionidae
650 $aEnzymatic hydrolysis
650 $aGene expression
650 $aInsect pests
650 $aStarch
650 $aAmido
650 $aCurculionideo
650 $aHidrolise
650 $aPraga de planta
653 $aExpressão gênica
700 1 $aMACEDO, L. L. P.
700 1 $aAMORIM, T. M. L.
700 1 $aSANTOS, V. O.
700 1 $aFRAGOSO, R. da R.
700 1 $aLUCENA, W. A.
700 1 $aMENEGUIM, A. M.
700 1 $aVALENCIA-JIMENEZ, A.
700 1 $aENGLER, G.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
700 1 $aFREIRE, E. V. S. A.
773 $tGene$gv. 553, n. 1, p. 7-16, Dec. 2014.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
15/03/1999 |
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Data da última atualização: |
25/07/2017 |
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Autoria: |
ARAUJO FILHO, J. A. de; BARBOSA, T. M. L.; CARVALHO, F. C. de. |
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Afiliação: |
João Ambrósio de Araújo Filho, CNPC; Tânia Maria Leal Barbosa, Pós-graduação; Fabiano Cavalcante de Carvalho, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE. |
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Título: |
Sistema de produção silvopastoril para o semi-árido nordestino. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Sobral: Embrapa Caprinos, 1999. |
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Páginas: |
2 p. |
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Série: |
(Embrapa Caprinos. Pesquisa em Andamento, 29). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo da pesquisa consiste no desenvolvimento de sistemas de manejo sustentado da vegetação lenhosa da caatinga, com vistas ao atendimento da demanda dos pequenos produtores por forragem e lenha, proporcionando-lhes, tambem, uma alternativa de melhoria de renda. |
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Palavras-Chave: |
Agrosilvopastoral systems; Brasil; Ceara; Management; Manejo sustenatado; Nordeste; Pastorialism; Pernambuco; Piaui; Production system; Regiao semi-arida; Semi-Arido; Silvopastoril; Sistema; Sistema silvopastoril; Sustainability; Woody plant. |
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Thesagro: |
Caatinga; Forragem; Lenha; Pastagem; Planta lenhosa; Produção; Sistema de Produção; Vegetação. |
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Thesaurus NAL: |
Brazil; forage; fuelwood; pastures; Semiarid soils; silvopastoral systems; vegetation. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26644/1/PA-29.pdf
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Marc: |
LEADER 01641nam a2200541 a 4500
001 1515055
005 2017-07-25
008 1999 bl uuuu u0uu1 u #d
100 1 $aARAUJO FILHO, J. A. de
245 $aSistema de produção silvopastoril para o semi-árido nordestino.
260 $aSobral: Embrapa Caprinos$c1999
300 $a2 p.
490 $a(Embrapa Caprinos. Pesquisa em Andamento, 29).
520 $aO objetivo da pesquisa consiste no desenvolvimento de sistemas de manejo sustentado da vegetação lenhosa da caatinga, com vistas ao atendimento da demanda dos pequenos produtores por forragem e lenha, proporcionando-lhes, tambem, uma alternativa de melhoria de renda.
650 $aBrazil
650 $aforage
650 $afuelwood
650 $apastures
650 $aSemiarid soils
650 $asilvopastoral systems
650 $avegetation
650 $aCaatinga
650 $aForragem
650 $aLenha
650 $aPastagem
650 $aPlanta lenhosa
650 $aProdução
650 $aSistema de Produção
650 $aVegetação
653 $aAgrosilvopastoral systems
653 $aBrasil
653 $aCeara
653 $aManagement
653 $aManejo sustenatado
653 $aNordeste
653 $aPastorialism
653 $aPernambuco
653 $aPiaui
653 $aProduction system
653 $aRegiao semi-arida
653 $aSemi-Arido
653 $aSilvopastoril
653 $aSistema
653 $aSistema silvopastoril
653 $aSustainability
653 $aWoody plant
700 1 $aBARBOSA, T. M. L.
700 1 $aCARVALHO, F. C. de
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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