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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
03/12/2014 |
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Data da última atualização: |
03/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. |
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Afiliação: |
ROSANGELA BEVITORI, CNPAF; MARILIA BARROS OLIVEIRA, doutoranda UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; ANNA CRISTINA LANNA, CNPAF; RICARDO DIÓGENES DA SILVEIRA; SILVANA PETROFEZA, UFG. |
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Título: |
Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014. |
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ISSN: |
1676-5680 |
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DOI: |
http://dx.doi.org/10.4238/2014.November.27.7 |
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Idioma: |
Inglês |
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Conteúdo: |
Drought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice. |
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Thesagro: |
Arroz; Brusone; Doença de planta; Genética molecular; Oryza sativa; Seca. |
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Thesaurus Nal: |
Blast disease; Gene expression; Magnaporthe oryzae; Rice. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112900/1/gmr4195.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179634/1/gmr4195.pdf
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Marc: |
LEADER 02118naa a2200325 a 4500
001 2001427
005 2014-12-03
008 2014 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $ahttp://dx.doi.org/10.4238/2014.November.27.7$2DOI
100 1 $aBEVITORI, R.
245 $aSelection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought.$h[electronic resource]
260 $c2014
520 $aDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.
650 $aBlast disease
650 $aGene expression
650 $aMagnaporthe oryzae
650 $aRice
650 $aArroz
650 $aBrusone
650 $aDoença de planta
650 $aGenética molecular
650 $aOryza sativa
650 $aSeca
700 1 $aOLIVEIRA, M. B.
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aLANNA, A. C.
700 1 $aSILVEIRA, R. D. da
700 1 $aPETROFEZA, S.
773 $tGenetics and Molecular Research$gv. 13, n. 4, p. 9795-9805, 2014.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| 1. |  | BEVITORI, R.; OLIVEIRA, M. B.; GROSSI-DE-SÁ, M. F.; LANNA, A. C.; SILVEIRA, R. D. da; PETROFEZA, S. Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Genetics and Molecular Research, v. 13, n. 4, p. 9795-9805, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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