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| 1. |  | BIANCO, L. F.; CARVALHO, J. F. C.; TERASSI, F. S.; SEINO, Y. W.; TREVIZAN, F. H.; ONOFRE, E.; NEUMAIER, N.; OLIVEIRA, M. C. N. de; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Crescimento de soja geneticamente modificada com os genes AtDREB1A e AtDREB2A sob déficit hídrico. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 108-114. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello. Disponível em: .| Biblioteca(s): Embrapa Soja. |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
20/12/2017 |
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Data da última atualização: |
21/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PEREIRA, C. E. R.; VANNUCCI, F. A.; GABARDO, M. de P.; SANTOS, L. F. dos; MORES, N.; GUEDES, R. M. C. |
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Afiliação: |
Carlos E. R. Pereira, Departamento de Clínica e Cirurgia/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Fabio A. Vannucci, Laboratório de Microbiologia Veterinária; Michelle de P. Gabardo, Departamento de Clínica e Cirurgia/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Lucas F. dos Santos; NELSON MORES, CNPSA; Roberto M. C. Guedes, Departamento de Clínica e Cirurgia/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG. |
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Título: |
Mycoplasma hyorhinis infection in early cases of mycoplasmal pneumonia in swine and evaluation of diagnostic assays. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1057-1063, outubro 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Infecção por Mycoplasma hyorhinis em casos precoces de pneumonia micoplásmica em suínos e comparação entre técnicas diagnósticas. |
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Conteúdo: |
Mycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This finding indicates that the
association of M. hyorhinis and viral infection in nursery pigs likely starts due to a viral immunosuppressive condition. MenosMycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This findin... Mostrar Tudo |
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Palavras-Chave: |
Mmunohistochemistry; Mycoplasmal pneumonia; Swines. |
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Thesaurus NAL: |
Fluorescence in situ hybridization; Mycoplasma hyopneumoniae; Mycoplasma hyorhinis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169437/1/Mycoplasma-hyorhinis-infection.pdf
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Marc: |
LEADER 02663naa a2200265 a 4500
001 2088004
005 2018-02-21
008 2017 bl uuuu u00u1 u #d
100 1 $aPEREIRA, C. E. R.
245 $aMycoplasma hyorhinis infection in early cases of mycoplasmal pneumonia in swine and evaluation of diagnostic assays.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Infecção por Mycoplasma hyorhinis em casos precoces de pneumonia micoplásmica em suínos e comparação entre técnicas diagnósticas.
520 $aMycoplasmal pneumonia is an important disease in the pig industry. Due to the controversial role of Mycoplasma hyorhinis in this disease, confirmation of the presence of this bacterium and the identification of its roles in respiratory disease remain major challenges. The objectives of this study were to evaluate the presence of M. hyorhinis in early cases of mycoplasmal pneumonia and to determine the usefulness of fluorescent in situ hybridization (FISH) for the diagnosis of respiratory mycoplasmosis in naturally infected pigs. Ninety M. hyopneumoniae and/or M. hyorhinis-infected lung tissue samples based on diagnostic mosaic (DM) were used. The average age of the animals was 116 and 57 days (P<0.01) for groups 1 (positive-M. hyopneumoniae only) and 2 (positive-M. hyorhinis only), respectively. These findings suggest that development of lesions caused by M. hyorhinis occurs earlier than for M. hyopneumoniae. Using the DM as the gold standard, the sensitivity and specificity of FISH for M. hyopneumoniae were 75 and 100%, respectively, and were 40 and 73.3% for the immunohistochemistry (IHC). The sensitivity and specificity of FISH for M. hyorhinis were 76.7 and 100%, respectively. These findings demonstrate that FISH can be a useful tool for diagnosing mycoplasmosis. Viral antigens (PCV2 or influenza A) were detected in 53.3% (16/30) of the samples in group 2 (M. hyorhinis-PCR positive) and 13.3% (4/30) of the samples in group 1 (M. hyopneumoniae-PCR positive). This finding indicates that the association of M. hyorhinis and viral infection in nursery pigs likely starts due to a viral immunosuppressive condition.
650 $aFluorescence in situ hybridization
650 $aMycoplasma hyopneumoniae
650 $aMycoplasma hyorhinis
653 $aMmunohistochemistry
653 $aMycoplasmal pneumonia
653 $aSwines
700 1 $aVANNUCCI, F. A.
700 1 $aGABARDO, M. de P.
700 1 $aSANTOS, L. F. dos
700 1 $aMORES, N.
700 1 $aGUEDES, R. M. C.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n, 10, p. 1057-1063, outubro 2017.
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Embrapa Suínos e Aves (CNPSA) |
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