Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
22/08/2012 |
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Data da última atualização: |
16/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NICIURA, S. C. M.; VERÍSSIMO, C. J.; GRAMBONI, J. G. G.; ROCHA, M. I. P.; MELLO, S. C. de; BARBOSA, C. M. P.; CHIEBAO, D. P.; CARDOSO, D.; SILVA, G. S.; OTSUK, I. P.; PEREIRA, J. R.; AMBROSIO, L. A.; NARDON, R. F.; UENO, T. E. H.; MOLENTO, M. B. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CECÍLIA JOSÉ VERÍSSIMO, INSTITUTO DE ZOOTECNIA/NOVA ODESSA; JULIANA GRACIELLE GONZAGA GROMBONI; MARINA IBELLI PEREIRA, UFSCAR/SÃO CARLOS, SP; SUELEN SCARPA DE MELLO; CRISTINA MARIA PACHECO BARBOSA, APTA PÓLO REGIONAL SUDOESTE PAULISTA/ITAPETININGA; DANIELA PONTES CHIEBAO, APTA PÓLO REGIONAL SUDOESTE PAULISTA/SOROCABA; DANIEL CARDOSO, Apta; GIANE SERAFIM SILVA, Apta; IVANI POZAR OTSUK, Instituto de Zootecnia; JOSÉ ROBERTO PEREIRA, Apta; LUIS ALBERTO AMBROSIO, Instituto de Zootecnia; ROMEU FERNANDES NARDON, Apta; TATIANA EVELYN HAYAMA UENO, Apta; MARCELO BELTRÃO MOLENTO, Universidade Federal do Paraná. |
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Título: |
F200Y polymorphism in the B-tubulin gene in field isolates of Haemonchus contortus and risk factors of sheep flock management practices related to anthelmintic resistance. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 190, n. 3-4, p. 608-612, dec. 2012. |
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DOI: |
10.1016/j.vetpar.2012.07.016 |
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Idioma: |
Inglês |
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Conteúdo: |
Haemonchus contortus is the most prevalent and pathogenic nematode of sheep in tropical areas. The objectives of this study were to assess the frequency of the F200Y polymorphism on the ?-tubulin gene in third-stage larvae of H. contortus from 33 sheep flocks in São Paulo state, Brazil, and to associate this frequency to risk factors based on farm management practices. The resistance allele frequency varied from 9 to 74%, and the resistance genotype frequency varied from 0 to 66.7%. Resistance genotype frequencies higher than 40% were associated with multiple risk factors - new sheep farming enterprises, the absence of farm records, the use of Dorper and Suffolk breeds, rotational grazing, the lack of wetlands on farms, pasture sharing with cattle or horses, frequent incorporation of animals into the flock, semi-intensive farming systems, whole-flock treatment, failure to use the FAMACHA method, lack of the dose-and-move practice, anthelmintic rotation after each application, visual estimation of animal weight for treatment, and lack of drug combination use. It can be concluded that genotyping the F200Y polymorphism can be used to monitor the resistance in sheep flocks and the knowledge of management strategies at the farm level is important to identify drug resistance related factors. |
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Palavras-Chave: |
Management practices; Nematode; PCR. |
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Thesagro: |
DNA. |
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Thesaurus NAL: |
benzimidazole; drug resistance. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02445naa a2200373 a 4500
001 1931915
005 2023-02-16
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vetpar.2012.07.016$2DOI
100 1 $aNICIURA, S. C. M.
245 $aF200Y polymorphism in the B-tubulin gene in field isolates of Haemonchus contortus and risk factors of sheep flock management practices related to anthelmintic resistance.$h[electronic resource]
260 $c2012
520 $aHaemonchus contortus is the most prevalent and pathogenic nematode of sheep in tropical areas. The objectives of this study were to assess the frequency of the F200Y polymorphism on the ?-tubulin gene in third-stage larvae of H. contortus from 33 sheep flocks in São Paulo state, Brazil, and to associate this frequency to risk factors based on farm management practices. The resistance allele frequency varied from 9 to 74%, and the resistance genotype frequency varied from 0 to 66.7%. Resistance genotype frequencies higher than 40% were associated with multiple risk factors - new sheep farming enterprises, the absence of farm records, the use of Dorper and Suffolk breeds, rotational grazing, the lack of wetlands on farms, pasture sharing with cattle or horses, frequent incorporation of animals into the flock, semi-intensive farming systems, whole-flock treatment, failure to use the FAMACHA method, lack of the dose-and-move practice, anthelmintic rotation after each application, visual estimation of animal weight for treatment, and lack of drug combination use. It can be concluded that genotyping the F200Y polymorphism can be used to monitor the resistance in sheep flocks and the knowledge of management strategies at the farm level is important to identify drug resistance related factors.
650 $abenzimidazole
650 $adrug resistance
650 $aDNA
653 $aManagement practices
653 $aNematode
653 $aPCR
700 1 $aVERÍSSIMO, C. J.
700 1 $aGRAMBONI, J. G. G.
700 1 $aROCHA, M. I. P.
700 1 $aMELLO, S. C. de
700 1 $aBARBOSA, C. M. P.
700 1 $aCHIEBAO, D. P.
700 1 $aCARDOSO, D.
700 1 $aSILVA, G. S.
700 1 $aOTSUK, I. P.
700 1 $aPEREIRA, J. R.
700 1 $aAMBROSIO, L. A.
700 1 $aNARDON, R. F.
700 1 $aUENO, T. E. H.
700 1 $aMOLENTO, M. B.
773 $tVeterinary Parasitology$gv. 190, n. 3-4, p. 608-612, dec. 2012.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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