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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
17/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ALVAREZ-PIZARRO, J. C.; GOMES FILHO, E.; PRISCO, J. T.; GROSSI DE SA, M. F.; OLIVEIRA NETO, O. B. de; FRAGOSO, R. da R. |
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Afiliação: |
MARIA FATIMA GROSSI DE SA, CENARGEN; RODRIGO DA ROCHA FRAGOSO, CPAC. |
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Título: |
Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Biologia Plantarum, v. 58, n. 3, p. 507-514, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants. |
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Palavras-Chave: |
Nitrato de Amonia. |
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Thesagro: |
Fertilizante nitrogenado; Potássio; Sorghum Bicolor; Sorgo. |
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Thesaurus NAL: |
Ammonium nitrate; Nitrate fertilizers; Potassium. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01933naa a2200277 a 4500
001 2003022
005 2014-12-17
008 2014 bl uuuu u00u1 u #d
100 1 $aALVAREZ-PIZARRO, J. C.
245 $aPlasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources.
260 $c2014
520 $aAbstract: We studied the influence of inorganic nitrogen sources (NO3- or NH4+) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4+-fed roots compared with NO3--fed roots. The optimum pH of the enzyme was slightly lower in NO3- -fed roots than in NH4+ -fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 were increased in roots grown under NH4+. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3- or NH4+, but it was significantly reduced by vanadate in NH4+-grown plants.
650 $aAmmonium nitrate
650 $aNitrate fertilizers
650 $aPotassium
650 $aFertilizante nitrogenado
650 $aPotássio
650 $aSorghum Bicolor
650 $aSorgo
653 $aNitrato de Amonia
700 1 $aGOMES FILHO, E.
700 1 $aPRISCO, J. T.
700 1 $aGROSSI DE SA, M. F.
700 1 $aOLIVEIRA NETO, O. B. de
700 1 $aFRAGOSO, R. da R.
773 $tBiologia Plantarum$gv. 58, n. 3, p. 507-514, 2014.
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