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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
22/12/1999 |
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Data da última atualização: |
12/03/2010 |
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Autoria: |
COLNAGO, L. A.; OPELLA, S. J. |
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Afiliação: |
EMBRAPA-CNPDIA; University of Pennsylvania. |
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Título: |
Structure and dynamics of filamentous bacteriophages by solid state NMR. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DE FISICA DA MATERIA CONDENSADA, 22., maio 1993, Caxambu, MG. Programa e resumos... Sao Paulo: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1993. |
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Páginas: |
p.9. |
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Idioma: |
Inglês |
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Conteúdo: |
High resolution multinuclear and multidimensional solution NMR is an alternative to X-ray diffraction to solve the structure of proteins up to 30KDa. For large structures, solid state NMR has been used in several cases. The structural information obtained from solid state NMR spectroscopy is due to the angular dependence of the dipolar and quadrupolar interactions and chemical shift anisotropy. Solid state NMR was used to solve the strucuture and dynamics of filamentous bacteriophages class 1(M13, fd and f1) and class 2(pF1). The viruses are very usefull in molecular biology, as vectors. A new application of these bacteriophages resides in a fusion of peptides or proteins to theirs coat proteins, to create epitope libraries, vaccines, etc. We have been using N15, multidimensional solid state NMR to solve the structure of these fused peptides in the coat protein of gene VIII. Preliminary results show that only part of the fused peptides are structured in the virus. |
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Palavras-Chave: |
Bacteriophage; Filamentous; NMR; Solid state; Structure. |
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Thesaurus NAL: |
dynamics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01658naa a2200217 a 4500
001 1026764
005 2010-03-12
008 1993 bl --- 0-- u #d
100 1 $aCOLNAGO, L. A.
245 $aStructure and dynamics of filamentous bacteriophages by solid state NMR.
260 $c1993
300 $ap.9.
520 $aHigh resolution multinuclear and multidimensional solution NMR is an alternative to X-ray diffraction to solve the structure of proteins up to 30KDa. For large structures, solid state NMR has been used in several cases. The structural information obtained from solid state NMR spectroscopy is due to the angular dependence of the dipolar and quadrupolar interactions and chemical shift anisotropy. Solid state NMR was used to solve the strucuture and dynamics of filamentous bacteriophages class 1(M13, fd and f1) and class 2(pF1). The viruses are very usefull in molecular biology, as vectors. A new application of these bacteriophages resides in a fusion of peptides or proteins to theirs coat proteins, to create epitope libraries, vaccines, etc. We have been using N15, multidimensional solid state NMR to solve the structure of these fused peptides in the coat protein of gene VIII. Preliminary results show that only part of the fused peptides are structured in the virus.
650 $adynamics
653 $aBacteriophage
653 $aFilamentous
653 $aNMR
653 $aSolid state
653 $aStructure
700 1 $aOPELLA, S. J.
773 $tIn: REUNIAO ANUAL DE FISICA DA MATERIA CONDENSADA, 22., maio 1993, Caxambu, MG. Programa e resumos... Sao Paulo: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1993.
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