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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
31/08/2012 |
Data da última atualização: |
02/08/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MOREIRA, A. A.; SANTOS, R. F.; MANDARINO, J. M. G.; VARÉA, G. S.; IDA, E. I.; RIBEIRO, M. L. L. |
Afiliação: |
AMANDA A. MOREIRA, UEL; RAFAEL F. SANTOS, UEL; JOSE MARCOS GONTIJO MANDARINO, CNPSO; GENI S. VARÉA, UEL - Professora; ELZA I. IDA, UEL; MARA L. L. RIBEIRO, UEL. |
Título: |
Conversion of isoflavone glucosides to aglycones in whole soybean flour thermally treated and with endogenous B-glucosidase of soybean. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: [proceedings]. Foz do Iguaçu: IUFoST, 2012. 1 CD-ROM. |
Idioma: |
Inglês |
Conteúdo: |
The B-glucosidase hydrolyze isoflavone glucosides releasing aglycones. Its application in the food industry is relevant to the production of soybean foods with higher levels of isoflavone aglycones, with benefits for human health. The objective of this study was to apply endogenous B-glucosidase of soybean in whole soybean flour (WSF) and evaluate the conversion of isoflavone glucosides to aglycones. The B-glucosidase was fractionated by 40-85% ammonium sulfate saturation, concentrated by ultrafiltration (MWCO 100 kDa) and was applied in WSF thermally treated under different conditions. WSF without heat treatment and without application of enzyme was used as control (WSFC). Thermal pretreatment was performed in WSFC for 1h at 100°C (WSF100) or autoclaved for 30min at 121°C (WSF121). In these treatments were added 10U or 50U of B-glucosidase and incubated at 30°C for 2 or 6h. The aglycones content was determined by HPLC and the results were expressed as μg g-1 of samples. Thermal pretreatment increased the aglycone content of WSF100 and WSF121 in 2.6 and 2.8 times, respectively, relative to WSFC. The application of 50U of -glucosidase for 6h at 30°C in and CSF100 and CSF121 increased aglycones content of 7.1 and 8.5 times, respectively, relative to WSFC. The -glucosidase was efficient in the conversion of isoflavone glucosides to aglycones in all treatments compared with the control. |
Palavras-Chave: |
Isoflavona. |
Thesagro: |
Soja; Tecnologia de alimento. |
Thesaurus NAL: |
Food technology; Isoflavones; Soybeans. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/65350/1/05324.pdf
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Marc: |
LEADER 02378nam a2200241 a 4500 001 1932726 005 2017-08-02 008 2012 bl uuuu u00u1 u #d 100 1 $aMOREIRA, A. A. 245 $aConversion of isoflavone glucosides to aglycones in whole soybean flour thermally treated and with endogenous B-glucosidase of soybean. 260 $aIn: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: [proceedings]. Foz do Iguaçu: IUFoST, 2012. 1 CD-ROM.$c2012 520 $aThe B-glucosidase hydrolyze isoflavone glucosides releasing aglycones. Its application in the food industry is relevant to the production of soybean foods with higher levels of isoflavone aglycones, with benefits for human health. The objective of this study was to apply endogenous B-glucosidase of soybean in whole soybean flour (WSF) and evaluate the conversion of isoflavone glucosides to aglycones. The B-glucosidase was fractionated by 40-85% ammonium sulfate saturation, concentrated by ultrafiltration (MWCO 100 kDa) and was applied in WSF thermally treated under different conditions. WSF without heat treatment and without application of enzyme was used as control (WSFC). Thermal pretreatment was performed in WSFC for 1h at 100°C (WSF100) or autoclaved for 30min at 121°C (WSF121). In these treatments were added 10U or 50U of B-glucosidase and incubated at 30°C for 2 or 6h. The aglycones content was determined by HPLC and the results were expressed as μg g-1 of samples. Thermal pretreatment increased the aglycone content of WSF100 and WSF121 in 2.6 and 2.8 times, respectively, relative to WSFC. The application of 50U of -glucosidase for 6h at 30°C in and CSF100 and CSF121 increased aglycones content of 7.1 and 8.5 times, respectively, relative to WSFC. The -glucosidase was efficient in the conversion of isoflavone glucosides to aglycones in all treatments compared with the control. 650 $aFood technology 650 $aIsoflavones 650 $aSoybeans 650 $aSoja 650 $aTecnologia de alimento 653 $aIsoflavona 700 1 $aSANTOS, R. F. 700 1 $aMANDARINO, J. M. G. 700 1 $aVARÉA, G. S. 700 1 $aIDA, E. I. 700 1 $aRIBEIRO, M. L. L.
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Embrapa Soja (CNPSO) |
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