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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/04/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SABINO, Y. N. V.; FOCHAT, R. C.; LIMA, J. C. F.; RIBEIRO, M. T.; ARCURI, P. B.; CARNEIRO, J. da C.; MACHADO, M. A.; REIS, D. R. de L.; MACHADO, A. B. F.; HÚNGARO, H. M.; RIBEIRO, J. B.; PAIVA, A. D. |
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Afiliação: |
Yasmin Neves Vieira Sabino, UFJF; Romário Costa Fochat, UFJF; JUNIOR CESAR FERNANDES LIMA, CNPGL; MARLICE TEIXEIRA RIBEIRO, CNPGL; PEDRO BRAGA ARCURI, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; Alessandra Barbosa Ferreira Machado, UFTM, Uberaba; Humberto Moreira Húngaro, UFJF; JOAO BATISTA RIBEIRO, CNPGL; Aline Dias Paiva, UFTM, Uberaba. |
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Título: |
Antibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Annals of Microbiology, v. 69, p. 131-138, 2019. |
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DOI: |
10.1007/s13213-018-1407-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem. |
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Palavras-Chave: |
Antibacterial activity; Bacteriocin; LanC; Lantibiotic; Streptococcus sp. |
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Thesagro: |
Rúmen. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02399naa a2200337 a 4500
001 2108297
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s13213-018-1407-2$2DOI
100 1 $aSABINO, Y. N. V.
245 $aAntibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid.$h[electronic resource]
260 $c2019
520 $aAbstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem.
650 $aRúmen
653 $aAntibacterial activity
653 $aBacteriocin
653 $aLanC
653 $aLantibiotic
653 $aStreptococcus sp
700 1 $aFOCHAT, R. C.
700 1 $aLIMA, J. C. F.
700 1 $aRIBEIRO, M. T.
700 1 $aARCURI, P. B.
700 1 $aCARNEIRO, J. da C.
700 1 $aMACHADO, M. A.
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, A. B. F.
700 1 $aHÚNGARO, H. M.
700 1 $aRIBEIRO, J. B.
700 1 $aPAIVA, A. D.
773 $tAnnals of Microbiology$gv. 69, p. 131-138, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
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Registro completo
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Biblioteca(s): |
Catálogo Coletivo de Periódicos Embrapa; Embrapa Amazônia Oriental. |
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Identificador: |
503 |
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Data corrente: |
09/05/2002 |
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Data da última atualização: |
12/05/2015 |
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Código do título: |
0901372 |
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ISSN: |
0100-1922 |
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Código CCN: |
003459-2 |
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Título e Subtítulo: |
ANAIS DA BIBLIOTECA NACIONAL |
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Título alternativo: |
ANAES DA BIBLIOTHECA NACIONAL |
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Entidade: |
Biblioteca Nacional do Rio de Janeiro |
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Local de publicação: |
Rio De Janeiro, RJ |
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Periodicidade: |
Anual |
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Inicio de publicação: |
1876 |
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Coleções da unidade: |
Embrapa Amazônia Oriental
1883 11; 1884/85 12; 1886 14(1-2); 1887/88 15(1/2); 1889/90 16(1-2); 1891/92 17; 1896 18; 1898 20; 1899 21; 1900 22; 1901 23; 1903 25; 1904 26; 1905 27; 1906 28; 1907 29; 1908 30; 1909 31; 1910 32; 1911 33; 1912 34; 1913 35; 1914 36; 1915 37; 1916 38; 1917 39; 1918 40; 1919 41; 1920 42; 1924 46; 1925 47; 1927 49; 1928 50; 1929 51; 1930 52; 1931 53; 1932 54; 1933 55; 1934 56; 1935 57; 1936 58; 1937 59; 1938 60; 1948 66, 67; 1949 68; 1950 69, 70; 1951 71, 72; 1953 74; 1954 73; 1955 75; 1956 76; 1957 77; 1958 78; 1959 79; 1960 80; 1962 82; 1963 83; 1964 84; 1965 85; 1966 86; 1967 87; 1968 88; 1969 89; 1970 90; 1971 91; 1972 92(3,6-8); 1973 93; 1974 94; 1975 95(1-2); 1976 96; 1977 97; 1978 98; 1980 100; 1981 101; 1982 102; 1983 103; 1985 105; 1986 106; 1987 107; 1988 108; 1989 109; 1990 110; 1991 111; 1992 112; 1993 113; 1994 114
Classificação: 981.05A6 |
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