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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
22/02/2017 |
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Data da última atualização: |
03/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
FREITAS, R. T. da; PAIVA, R.; SALES, T. S.; SILVA, D. P. C. da; REIS, M. V. dos; SOUZA, A. C. de; ROSA, S. D. V. F. da. |
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Afiliação: |
RODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC. |
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Título: |
Cryopreservation of Coffea arabica L. zygotic embryos by vitrification. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Notulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption. |
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Palavras-Chave: |
Liquid nitrogen; PVS2; TTC test. |
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Thesaurus NAL: |
Ex situ conservation; Histology; Thawing. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156422/1/Cryopreservation-of-Coffea-arabica.pdf
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Marc: |
LEADER 01833naa a2200265 a 4500
001 2065188
005 2017-03-03
008 2016 bl uuuu u00u1 u #d
100 1 $aFREITAS, R. T. da
245 $aCryopreservation of Coffea arabica L. zygotic embryos by vitrification.$h[electronic resource]
260 $c2016
520 $aAs a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.
650 $aEx situ conservation
650 $aHistology
650 $aThawing
653 $aLiquid nitrogen
653 $aPVS2
653 $aTTC test
700 1 $aPAIVA, R.
700 1 $aSALES, T. S.
700 1 $aSILVA, D. P. C. da
700 1 $aREIS, M. V. dos
700 1 $aSOUZA, A. C. de
700 1 $aROSA, S. D. V. F. da
773 $tNotulac Botanicae Horti Agrobotanici Cluj-Napoca$gv. 44, n. 2, p. 445-451, Dec. 2016.
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Registro original: |
Embrapa Café (CNPCa) |
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