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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
25/04/2024 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
AQUINO, S. O. de; CARNEIRO, F. de A.; RÊGO, E. C. S.; ALVES, G. S. C.; MARRACCINI. P.; ANDRADE, A. C. |
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Afiliação: |
S. O. DE AQUINO, UFLA; F. DE A. CARNEIRO, UCB; E. C. S. RÊGO; G. S. C. ALVES, UFLA; P. MARRACCINI, CIRAD UMR AGAP; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
Functional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-46. |
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Idioma: |
Inglês |
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Conteúdo: |
In many higher plants, DREB genes were shown to be involved in the transduction pathways of abiotic stress. Previous results showed that CcDREB1D gene expression increased under drought stress in leaves of drought-tolerant clone 14 of Coffea canephora Conilon but not in those of the drought-susceptible clone 22. By sequencing the DREB1D promoter regions of these two clones, several nucleic polymorphisms (?single nucleotide polymorphism? [SNP] and insertion/deletion [INDELs]) were found. In order to know if these polymorphisms could explain the differences of DREB1D gene expression observed between the clones 14 and 22, 5' deletions of several alleles of the CcDREB1D promoter regions were made and cloned independently into the binary vector pBI101 in order to analyze their ability to control the expression of the uidA reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This work shows preliminary results of DREB1D promoter constructs analyzed in leaves of N. tabacum. |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01675nam a2200181 a 4500
001 2041748
005 2024-04-25
008 2015 bl uuuu u00u1 u #d
100 1 $aAQUINO, S. O. de
245 $aFunctional analysis of CcDREB1D promoter region from haplotypes of Coffea canephora through genetic transformation of Nicotiana tabacum.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-46.$c2015
520 $aIn many higher plants, DREB genes were shown to be involved in the transduction pathways of abiotic stress. Previous results showed that CcDREB1D gene expression increased under drought stress in leaves of drought-tolerant clone 14 of Coffea canephora Conilon but not in those of the drought-susceptible clone 22. By sequencing the DREB1D promoter regions of these two clones, several nucleic polymorphisms (?single nucleotide polymorphism? [SNP] and insertion/deletion [INDELs]) were found. In order to know if these polymorphisms could explain the differences of DREB1D gene expression observed between the clones 14 and 22, 5' deletions of several alleles of the CcDREB1D promoter regions were made and cloned independently into the binary vector pBI101 in order to analyze their ability to control the expression of the uidA reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This work shows preliminary results of DREB1D promoter constructs analyzed in leaves of N. tabacum.
653 $aCoffee
700 1 $aCARNEIRO, F. de A.
700 1 $aRÊGO, E. C. S.
700 1 $aALVES, G. S. C.
700 1 $aMARRACCINI. P.
700 1 $aANDRADE, A. C.
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