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| 1. |  | BALBINOT JUNIOR, A. A.; VEIGA, M. da; FONSECA, J. A. da; VOGT, G. A.; ALBUQUERQUE, J. A.; COSTA, E. R. O. Aplicação de resíduo de reciclagem de papel em Cambissolo Háplico e seu efeito no solo e no cultivo de plantas. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 38, n. 1, p. 336-344, Jan./Feb. 2014.| Biblioteca(s): Embrapa Soja. |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Uva e Vinho. |
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Data corrente: |
14/03/2024 |
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Data da última atualização: |
14/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
FAJARDO, T. V. M.; GRYNBERG, P.; TOGAWA, R. C.; SILVA, J. M. F.; SILVA, F. N. da; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; PRISCILA GRYNBERG, Cenargen; ROBERTO COITI TOGAWA, Cenargen; JOÃO MARCOS FAGUNDES SILVA, INSTITUTE FOR INTEGRATIVE SYSTEMS BIOLOGY; FABIO NASCIMENTO DA SILVA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; OSMAR NICKEL, CNPUV. |
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Título: |
Analyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Tropical Plant Pathology, [online], March 2024. |
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DOI: |
https://doi.org/10.1007/s40858-024-00647-3 |
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Idioma: |
Inglês |
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Conteúdo: |
The long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses. MenosThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps... Mostrar Tudo |
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Palavras-Chave: |
High throughput sequencing; Vector. |
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Thesaurus NAL: |
Ampelovirus; Closterovirus; Vitivirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162846/1/s40858-024-00647-3.pdf
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Marc: |
LEADER 02312naa a2200253 a 4500
001 2162846
005 2024-03-14
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-024-00647-3$2DOI
100 1 $aFAJARDO, T. V. M.
245 $aAnalyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity.$h[electronic resource]
260 $c2024
520 $aThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses.
650 $aAmpelovirus
650 $aClosterovirus
650 $aVitivirus
653 $aHigh throughput sequencing
653 $aVector
700 1 $aGRYNBERG, P.
700 1 $aTOGAWA, R. C.
700 1 $aSILVA, J. M. F.
700 1 $aSILVA, F. N. da
700 1 $aNICKEL, O.
773 $tTropical Plant Pathology, [online], March 2024.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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